scholarly journals Phenotypic Changes of Calponin in Salivary Gland Myoepithelial Cells during Postnatal Development of Rats.

2000 ◽  
Vol 33 (2) ◽  
pp. 73-79 ◽  
Author(s):  
Miyako Namba ◽  
Kazuto Yamada ◽  
Wataru Kudeken ◽  
Gho Mizutani ◽  
Kouji Tsukitani ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Postnatal Development ◽  
Myoepithelial Cells ◽  
Phenotypic Changes

scholarly journals Immunohistological characteristics of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during ontogenesis

2003 ◽  
Vol 50 (4) ◽  
pp. 179-183
Author(s):  
Ivan Dozic ◽  
Miodrag Colic
Keyword(s):  
Extracellular Matrix ◽  
Monoclonal Antibodies ◽  
Salivary Gland ◽  
Postnatal Development ◽  
Basal Membrane ◽  
Myoepithelial Cells ◽  
Matrix Proteins ◽  
Submandibular Salivary Gland ◽  
Ductal Cells ◽  
Positive Reactivity

The aim of this study was to investigate the expression of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during postnatal development (1st, 30th,and 60th day) by using various monoclonal antibodies (mAbs) RMC-23 (specific for collagen),a6?1 (specific for laminin) and ED3 (specific for sialoadhezin). These components of extracellular matrix were detected. RMC-23 mAb showed strong positivity to the basal membranes of the ductal system (intercalated, striated and excretory ducts) and of intersticium. Increased expression in the basal membrane of acini during development of glands was noted. Similar immunoreactivity was shown by?mAb but the intersticium showed a negative reaction to 1a6 this antibody. Positive reactivity of ?1a6 mAb of epithelial ductal cells, particularly of the neonatal animals, was found. In contrast to ? 1a6 and RMC23 mAbs, ED3 mAb was increasingly expressed in the myoepithelial cells during ontogenesis. Our findings regarding the immunoreactivity of collagens and laminins are in accordance with the findings of other autors. The very interesting finding of sialoadhesin in myoepithelial cells of the rat submandibular salivary gland, which is not described in literature and needs further investigation. Our results suggest that adhesion molecules and extracellular matrix proteins have an important biochemical role during postnatal development of the submandibular salivary gland.

scholarly journals Ultrastructural analysis of glands located in the wall of the congenital fistulae of the lower lip of patients with Van der Woude syndrome

2003 ◽  
Vol 11 (3) ◽  
pp. 192-202 ◽  
Author(s):  
Mirian Aparecida Onofre ◽  
Heli Benedito Brosco ◽  
Rumio Taga
Keyword(s):  
Salivary Gland ◽  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Minor Salivary Gland ◽  
Myoepithelial Cells ◽  
Minor Salivary Glands ◽  
Van Der Woude Syndrome ◽  
Lower Lip ◽  
Transmission Electron ◽  
Congenital Fistula

The objective of the present study was to evaluate the glands of wall of congenital fistulae of the lower lip with the transmission electron microscope in order to characterize their microstructural pattern. Thin section of Araldite resin embedded congenital fistulae of the lower lip of four patients with Van der Woude syndrome from the Hospital de Reabilitação de Anomalias Craniofaciais da Universidade de São Paulo, Bauru, SP, were analyzed with a transmission electron microscope. The results showed that the glands were mostly made by typical mucous acini exhibiting, with certain frequency, myoepithelial cells surrounding them. In some of lobules, a few acini smaller than the typical mucous, showed granules of moderate electron density or containing a dense core or exhibiting small dense spherule and predominance granular material. These granules resemble to described recently by others in various human minor salivary glands. We concluded that glands associated with congenital fistula of lower lip of patients with Van der Woude syndrome, in spite of being located in vermilion border of the lip, showed at the transmission electron microscope characteristics of labial minor salivary gland, i.e, are mostly mucous with a few seromucous units, while typical seromucous demilunes are not present.

scholarly journals Pleomorphic adenoma of the upper lip: a case report and review of literature

2016 ◽  
Vol 4 (1) ◽  
pp. 8
Author(s):  
Rashmi Metgud ◽  
Bhardwaj Tina Neelesh ◽  
Saurabh Goel ◽  
Smitha Nail ◽  
Shrikant Patel ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Pleomorphic Adenoma ◽  
Unusual Case ◽  
Needle Aspiration ◽  
Myoepithelial Cells ◽  
Upper Lip ◽  
Epithelial Tumor ◽  
Needle Aspiration Cytology ◽  
Benign Mixed Tumor ◽  
Morphological Patterns

<p>Pleomorphic Adenoma is a benign epithelial tumor arising from the salivary gland. It is a benign mixed tumor composed of epithelial and myoepithelial cells arranged with various morphological patterns, demarcated from surrounding tissues by fibrous capsule. In this article, we report an unusual case of 30 year, male with a single, nodular swelling evident in the upper lip which was discovered during a routine dental procedure. Fine Needle Aspiration Cytology (FNAC) was planned for the patient, where a of cytological diagnosis of Pleomorphic Adenoma was rendered, which was confirmed by biopsy. Hence, this highlights, FNAC makes up an important diagnosing tool as the first line of investigation to distinguish between benign and malignant salivary gland neoplasms.</p>

scholarly journals Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors

2007 ◽  
Vol 18 (3) ◽  
pp. 192-197 ◽  
Author(s):  
Roberta Barroso Cavalcante ◽  
Fernanda Ferreira Lopes ◽  
Andréa Soares Ferreira ◽  
Roseana de Almeida Freitas ◽  
Lélia Batista de Souza
Keyword(s):  
Salivary Gland ◽  
Myoepithelial Cells ◽  
Salivary Gland Tumors ◽  
Low Grade ◽  
Tubular Structures ◽  
Immunohistochemical Markers ◽  
Adenoid Cystic ◽  
Peripheral Cells ◽  
Diffuse Distribution ◽  
Labeling Pattern

Myoepithelial cells present a complex immunophenotype, with the expression of proteins varying according to the stage of normal or neoplastic differentiation of the cell. In order to evaluate the immunohistochemical markers expressed by these cells, a panel of antibodies composed of vimentin, calponin and HHF-35 was applied to 28 salivary gland tumors. The results demonstrated a higher percent sensitivity of vimentin and calponin compared to HHF-35. However, calponin and HHF-35 presented a focal labeling pattern in contrast with the diffuse distribution of vimentin. The cells predominantly stained by all tested antibodies included nonluminal cells in duct-like and tubular structures, such as those seen in pleomorphic adenomas and adenoid cystic carcinomas, as well as cells in the cords and nests of polymorphous low-grade adenocarcinomas and peripheral cells of sheets and nests of myoepitheliomas. In conclusion, the combination of calponin and vimentin is suggested for the identification of myoepithelial cells in salivary gland tumors.

Cytodifferentiation to Serous and Myoepithelial Cells in Salivary Gland and Salivary Gland Type Tumors

1977 ◽  
Vol 7 (2) ◽  
pp. 73-84 ◽  
Author(s):  
TORU KAMEYA ◽  
YUKIO SHIMOSATO ◽  
TOSHIYUKI TAKEUCHI ◽  
SHUICHI SASADAIRA
Keyword(s):  
Salivary Gland ◽  
Myoepithelial Cells ◽  
Gland Type

Expression of TdT in Myoepithelial Cells: Investigation in Breasts, Sweat Glands, and Salivary Lesions Emphasizing the Never-Documented Immunohistochemical Findings

2020 ◽  
Vol 28 (7) ◽  
pp. 711-720
Author(s):  
Jun Zhou ◽  
Haimin Xu ◽  
Hong Zeng ◽  
Hongjun Ma ◽  
Jingjing Yu ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Myoepithelial Cells ◽  
Cross Reactivity ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Sweat Glands ◽  
Routine Practice ◽  
Cell Adenoma ◽  
Normal Salivary Gland ◽  
Hidradenoma Papilliferum ◽  
Myoepithelial Differentiation

Background. The expression of terminal deoxynucleotidyl transferase (TdT) in myoepithelial cells (MECs) within the breast was recently incidentally observed in our routine practice. This study aimed to elucidate the expression of TdT in MECs. Methods. TdT immunostaining was performed on 180 mammary, 89 cutaneous, and 94 salivary tissues or lesions. Other myoepithelial markers, including P63, calponin, and SMA as well as double staining for TdT and calponin, were also evaluated in some cases. Selected lesions with basal or myoid differentiation were also included in the investigation. Results. MECs were positive for TdT in mammary lesions that contained MECs (132/135) but negative when they did not contain MECs (45/45). MECs in sweat glands (24/30) and their neoplastic counterparts, including those in hidradenoma papilliferum (2/9), spiradenoma (6/6), and cutaneous mixed tumor (9/9), showed weak to moderate TdT positivity. MECs were variably immunolabeled for TdT in salivary or salivary gland–type tumors with myoepithelial differentiation (pleomorphic adenoma, 24/25; basal cell adenoma, 6/7; adenoid cystic carcinoma, 7/7; Warthin tumor, 0/6; mucoepidermoid carcinoma, 0/8; acinic cell carcinoma, 0/4), but MECs in normal salivary gland barely stained for TdT (30/32). Conclusions. Our findings indicate that TdT may be eligible as an additional auxiliary immunohistochemical marker as P63, but not a surrogate, to identify the MECs in the breast with limited cross-reactivity, particularly in lesions with a prominent proportion of MECs. Positivity for TdT, along with other relevant markers, in a subset of sweat gland lesions and salivary tumors may contribute to their diagnosis.

The cell with a thousand faces: Detection of myoepithelial cells and their contributions in the cytological diagnosis of salivary gland tumors

2010 ◽  
Vol 40 (3) ◽  
pp. 220-227 ◽  
Author(s):  
Arzu Avcı ◽  
Ömer Günhan ◽  
Fulya Çakalaǧaoǧglu ◽  
Armagan Günal ◽  
Bülent Celasun
Keyword(s):  
Salivary Gland ◽  
Myoepithelial Cells ◽  
Salivary Gland Tumors ◽  
Cytological Diagnosis

scholarly journals Induction of other differentiation stages in neoplastic epithelial duct and myoepithelial cells from the human salivary gland grown in athymic nude mice

Cancer ◽  
1985 ◽  
Vol 55 (11) ◽  
pp. 2575-2583 ◽  
Author(s):  
Yoshio Hayashi ◽  
Tetsuo Yanagawa ◽  
Hideo Yoshida ◽  
Yoshiaki Yura ◽  
Toshiharu Nitta ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Nude Mice ◽  
Myoepithelial Cells ◽  
Athymic Nude Mice ◽  
Human Salivary Gland
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