A combined Alcian blue-PAS-ABC method for differential staining of mast cells.

1987 ◽  
Vol 20 (1) ◽  
pp. 101-104 ◽  
Author(s):  
NAOKI ARIZONO ◽  
OSAMU KORETO ◽  
YASUHIRO IWAI ◽  
RYOJI KUSHIMA ◽  
SUSUMU NAKAO ◽  
...  
Keyword(s):  
Mast Cells ◽  
Alcian Blue ◽  
Differential Staining ◽  
Abc Method

Mast Cell Heterogeneity in Human Lung Tissue

1989 ◽  
Vol 77 (3) ◽  
pp. 297-304 ◽  
Author(s):  
F. J. Van Overveld ◽  
L. A. M. J. Houben ◽  
F. E. M. Schmitz du Moulin ◽  
P. L. B. Bruijnzeel ◽  
J. A. M. Raaijmakers ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Lung Tissue ◽  
Human Lung ◽  
Immunoglobulin E ◽  
Prostaglandin D2 ◽  
Leukotriene C4 ◽  
Human Lung Tissue ◽  
No Release

1. In this study mast cells were found to comprise 2.1% of total cells recovered by enzymatic digestion of human lung tissue. 2. This mast cell population consisted of 79% formalin-sensitive, Alcian Blue-positive mast cells and 21% formalin-insensitive, Alcian Blue-positive mast cells. 3. By the use of centrifugal elutriation and subsequent Percoll gradient centrifugation, separate mixed cell populations could be obtained in which the mast cell constituents were either of the formalin-sensitive or -insensitive type. 4. Cell suspensions in which formalin-sensitive cells comprised 97% of mast cells contained approximately 1.34 pg of histamine per mast cell, whereas in preparations in which mast cells were 84% formalin-resistant the histamine content was approximately 4.17 pg of histamine per mast cell. 5. The histamine release upon anti-immunoglobulin E challenge of formalin-sensitive mast cells was greater than the release by formalin-insensitive mast cells. 6. After challenge with opsonized zymosan, only formalin-sensitive mast cells were able to release histamine. 7. Leukotriene C4 release was observed when formalin-sensitive mast cells were challenged with antiimmunoglobulin E. Formalin-insensitive mast cells showed no release of leukotriene C4. 8. Prostaglandin D2 release was observed when formalin-insensitive mast cells were challenged with antiimmunoglobulin E. Formalin-sensitive mast cells showed no release of prostaglandin D2.

scholarly journals Abnormal mast cell granules in the beige (Chédiak-Higashi syndrome) mouse.

1975 ◽  
Vol 23 (2) ◽  
pp. 117-122 ◽  
Author(s):  
E Y Chi ◽  
D Lagunoff
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Toluidine Blue ◽  
Ruthenium Red ◽  
Chediak Higashi Syndrome

Mast cells of beige (C57BL/6J) (bg-j/bg-j) mice were examined histochemically and ultrastructurally. Mast cell granules in the beige mice were markedly enlarged and irregular in shape. Granule contents stained uniformly with acidified toluidine blue, but with ruthenium red and Alcian Blue-safranin, two components were evident. The rims of the abnormal granules stained with ruthenium red and with Alcian Blue; the centers of the granules were clear with ruthenium red and stained with safranin. Mast cell granules thus represent another abnormal organelle in the Chédiak-Higashi syndrome.

scholarly journals Fibroblasts induce heparin synthesis in chondroitin sulfate E containing human bone marrow-derived mast cells

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1188-1195
Author(s):  
L Gilead ◽  
O Bibi ◽  
E Razin
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Chondroitin Sulfate ◽  
Alcian Blue ◽  
Proliferation Rate ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Thymidine Uptake ◽  
Cell Monolayers ◽  
Chondroitin Sulfate E

Human bone marrow-derived mast cells (hBMMCs), differentiated in vitro in suspension culture and under the influence of human peripheral blood mononuclear cells conditioned medium (hCM), were tested for their response to recombinant human interleukin-3 (rhIL-3) and for their behavior in different microenvironments. The hBMMCs were incubated in the presence of rhIL-3 and the changes in their proliferation rate were determined. Recombinant hIL-3 induced a more than sixfold increase in 3H-thymidine uptake into the hBMMC DNA in a dose-dependent manner. Human CM used as a control for proliferation response induced a more than eightfold maximal proliferation rate increase. Rabbit anti-rhIL-3 completely inhibited hBMMC 3H-thymidine uptake induced by rhIL-3 and decreased the hCM-induced proliferation by approximately 50%. These hBMMCs were cocultured with four different mytomicin C-treated cell monolayers and assayed for phenotypic changes. After only 2 days in coculture with either embryonic mouse skin-derived fibroblasts (MESFs) or human skin-derived fibroblasts (HSFs), a marked increase in granule number and density was noted on staining with toluidine blue. Mast cells that initially stained alcian blue+/safranin- at day 0 of coculture became alcian blue+/safranin+ during the coculture period. Human BMMC proteoglycan synthesis shifted from approximately 85% chondroitin sulfate E to approximately 60% heparin within 14 to 19 days of coculture with the MESF monolayer and to approximately 50% heparin within 19 days of coculture with the HSF monolayer. None of the above- mentioned changes were noted in cocultures of hBMMCs with 3T3 cell line fibroblast monolayers or in cocultures with bovine vascular endothelium (BVE) cell monolayers. These results demonstrate microenvironmental effects exerted by the MESF and HSF monolayers on IL-3-dependent hBMMCs similar to those reported in the conversion of murine mast cell phenotype.

scholarly journals The presence of mast cell precursors in rat peripheral blood

Blood ◽  
1981 ◽  
Vol 58 (3) ◽  
pp. 544-551 ◽  
Author(s):  
D Zucker-Franklin ◽  
G Grusky ◽  
N Hirayama ◽  
E Schnipper
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Peripheral Blood ◽  
Periodic Acid ◽  
Phenotypic Expression ◽  
Soft Agar ◽  
Sulfated Polysaccharides ◽  
Periodic Acid Schiff ◽  
Agar Culture

Abstract Soft agar culture of mononuclear cell fractions prepared from rat peripheral blood yielded numerous colonies consisting of mast cells. The mast cell nature of the cells was established by ultrastructural and histochemical analyses as well as by the demonstration the the colonies contained histamine and that the cells possessed receptors for the Fc component of IgE. Stringent criteria for the distinction of mast cells from monocytes/macrophages that could have metachromatic inclusions were applied. The alcian-blue-safranin technique delineated the maturation of mast cell granules by showing the loss of alcian-blue and increase in safranin-positive organelles presumed to reflect the increase in N-sulfated polysaccharides representing heparin. The mast cells exhibited low or absent reactions for peroxidase, alpha-naphthyl butyrate, periodic acid Schiff, and Sudan black reacting lipid, whereas macrophages stained in parallel were positive for these substances. Since it is known that extracellular conditions may cause variations in phenotypic expression, the observations have led to the hypothesis that mast cells and macrophages may have a common precursor.

An improved method for staining mouse mast cells

1989 ◽  
Vol 67 (1) ◽  
pp. 226-227 ◽  
Author(s):  
M. Novak ◽  
S. Nombrado
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Connective Tissue ◽  
Alcian Blue ◽  
Cell Types ◽  
Mucosal Mast Cell ◽  
Improved Method ◽  
Tissue Mast Cell ◽  
Pathological Conditions

An improved method for staining mouse mast cells with alcian blue is reported. The reaction differentiates between mucosal mast cell and connective tissue mast cell types, especially under pathological conditions.

scholarly journals Acid phosphatase reaction and Alcian Blue-Safranin staining of rat peritoneal mast cells.

1986 ◽  
Vol 19 (6) ◽  
pp. 761-765 ◽  
Author(s):  
SHIRO MIYASHITA ◽  
OSAMU KORETOU ◽  
YASUHIRO IWAI ◽  
NAOKI ARIZONO ◽  
OSAMU TAKEOKA
Keyword(s):  
Acid Phosphatase ◽  
Mast Cells ◽  
Alcian Blue ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
Acid Phosphatase Reaction

scholarly journals The presence of mast cell precursors in rat peripheral blood

Blood ◽  
1981 ◽  
Vol 58 (3) ◽  
pp. 544-551
Author(s):  
D Zucker-Franklin ◽  
G Grusky ◽  
N Hirayama ◽  
E Schnipper
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Peripheral Blood ◽  
Periodic Acid ◽  
Phenotypic Expression ◽  
Soft Agar ◽  
Sulfated Polysaccharides ◽  
Periodic Acid Schiff ◽  
Agar Culture

Soft agar culture of mononuclear cell fractions prepared from rat peripheral blood yielded numerous colonies consisting of mast cells. The mast cell nature of the cells was established by ultrastructural and histochemical analyses as well as by the demonstration the the colonies contained histamine and that the cells possessed receptors for the Fc component of IgE. Stringent criteria for the distinction of mast cells from monocytes/macrophages that could have metachromatic inclusions were applied. The alcian-blue-safranin technique delineated the maturation of mast cell granules by showing the loss of alcian-blue and increase in safranin-positive organelles presumed to reflect the increase in N-sulfated polysaccharides representing heparin. The mast cells exhibited low or absent reactions for peroxidase, alpha-naphthyl butyrate, periodic acid Schiff, and Sudan black reacting lipid, whereas macrophages stained in parallel were positive for these substances. Since it is known that extracellular conditions may cause variations in phenotypic expression, the observations have led to the hypothesis that mast cells and macrophages may have a common precursor.

scholarly journals SIMULTANEOUS DIFFERENTIAL STAINING BY A CATIONIC CARBOCYANINE DYE OF NUCLEIC ACIDS, PROTEINS AND CONJUGATED PROTEINS II. CARBOHYDRATE AND SULFATED CARBOHYDRATE-CONTAINING PROTEINS

1974 ◽  
Vol 22 (8) ◽  
pp. 774-781 ◽  
Author(s):  
MARIE R. GREEN ◽  
JULLIA V. PASTEWKA
Keyword(s):  
Mast Cells ◽  
Nucleic Acids ◽  
Hydrogen Ion ◽  
Differential Staining ◽  
Ion Concentrations ◽  
Pathologic Diagnosis ◽  
Carbocyanine Dye ◽  
Sulfated Carbohydrate ◽  
Color Reactions ◽  
Staining Time

The cationic carbocyanine dye l-ethyl-2-[3-(l-ethylnaphtho[l,2d]thiazolin-2-ylidene)-2-methylprolpenyl]-naphtho[1, 2d]thiazolium bromide stains several classes of macromolecules differentially in histologic sections. Most proteins are red at pH 4.3 and pink or unstained at pH 2.8. The caseins are blue at pH 4.3 and unstained at pH 2.8. Nuclei stain purple, mast cells stain red-purple, cartilage stains purple and mucoproteins stain blue-green at both hydrogen ion concentrations. The nature of some of the macromolecules involved in these color reactions has been determined by the use of chemical and enzymatic procedures and by staining films of glycosaminoglycuronoglycans, proteins and conjugated proteins. The method requires less than 1 hr staining time for most tissues; the stain is stable when kept in the dark and has the advantage of distinguishing several macromolecules in tissues simultaneously. The simplicity of the method and the ability to discriminate classes of macromolecules in tissues should make this stain valuable in pathologic diagnosis.

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