scholarly journals Inhibitory Effects of Platelet-Rich Plasma on Intervertebral Disc Degeneration: A Preclinical Study in a Rabbit Model

2015 ◽  
Vol 21 ◽  
pp. 1368-1375 ◽  
Author(s):  
Wangping Yin
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Platelet Rich Plasma ◽  
Rabbit Model ◽  
Preclinical Study ◽  
Inhibitory Effects

scholarly journals Efficacy of Platelet-Rich Plasma Containing Xenogenic Adipose Tissue-Derived Stromal Cells on Restoring Intervertebral Disc Degeneration: A Preclinical Study in a Rabbit Model

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Chao Ma ◽  
Ran Wang ◽  
Dingliang Zhao ◽  
Naikun Wang ◽  
Ying Han ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Stromal Cells ◽  
Signal Intensity ◽  
Intervertebral Disc Degeneration ◽  
Platelet Rich Plasma ◽  
Rabbit Model ◽  
Control Group ◽  
Arterial Blood

Objective. Platelet-rich plasma (PRP) containing multiple growth factors is a promising strategy for disc degeneration. Thus, this study hypothesizes that the combination of PRP and adipose tissue-derived stromal cells (ADSCs) may repair degenerative disc more effectively than using each one of them alone. Methods. The model of early intervertebral disc degeneration was induced by annular puncture in the New Zealand rabbit. Autologous PRP was extracted from fresh arterial blood by using two centrifugation techniques. ADSC was offered by the Center for Clinic Stem Cell Research. Four weeks after the first experiment, PRP or ADSCs or a combination of PRP and ADSCs was injected into the punctured intervertebral disc. Four weeks later, disc height and signal intensity on T2-weighted magnetic resonance imaging (MRI) were assessed. Results. One month after puncture, we detected relatively narrow discs and lower signal intensity in MRI T2-weighted images. At four weeks after injection, the PRP-ADSC group statistically significantly restored discs, compared with PRP, ADSCs, or negative control group. Conclusions. The combination of PRP and ADSCs shows an effective potential to restore degenerated intervertebral discs in the rabbit.

scholarly journals Effect of the adenovirus-mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model

2017 ◽  
Vol 16 (6) ◽  
pp. 9487-9493 ◽  
Author(s):  
Yuan Wang ◽  
Yong Yang ◽  
Jing-Chuan Sun ◽  
Qin-Jie Kong ◽  
Hai-Bo Wang ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Rabbit Model ◽  
Lumbar Intervertebral Disc

Efficacy of HYADD®4-G single intra-discal injections in a rabbit model of intervertebral disc degeneration

2019 ◽  
Vol 30 (4) ◽  
pp. 403-417 ◽  
Author(s):  
Atsuya Watanabe ◽  
Pierre Mainil-Varlet ◽  
Adeline Decambron ◽  
Caroline Aschinger ◽  
Antonella Schiavinato
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Rabbit Model

scholarly journals Multiscale and multimodal structure–function analysis of intervertebral disc degeneration in a rabbit model

2019 ◽  
Vol 27 (12) ◽  
pp. 1860-1869 ◽  
Author(s):  
B.G. Ashinsky ◽  
S.E. Gullbrand ◽  
E.D. Bonnevie ◽  
S.A. Mandalapu ◽  
C. Wang ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Structure Function ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Function Analysis ◽  
Rabbit Model

scholarly journals Antiaging Factor Klotho Retards the Progress of Intervertebral Disc Degeneration through the Toll-Like Receptor 4-NF-κB Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Fangfang Bi ◽  
Wenbo Liu ◽  
Zongtao Wu ◽  
Chen Ji ◽  
Cuicui Chang
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Acute Inflammation ◽  
Protective Effects ◽  
Toll Like Receptor ◽  
Inhibitory Effects ◽  
Toll Like Receptor 4 ◽  
Disc Injury ◽  
Anti Inflammation

Antiaging protein Klotho exhibits impressive properties of anti-inflammation, however is declined early after intervertebral disc injury, making Klotho restoration an attractive strategy of treating intervertebral disc inflammatory disorders. Here, we have found that Klotho is enriched in nucleus pulposus (NP) cells and Klotho overexpression attenuates H2O2-induced acute inflammation essentially via suppressing Toll-like receptor 4 (TLR4). The proinflammatory NF-κB signaling and cytokine expressions paralleled with Klotho repression and TLR4 elevation in both NP cells (H2O2 treatment) and rat intervertebral disc (needle puncture treatment). Overexpression of TLR4 downregulated expression of Klotho, whereas interfering TLR4 expression diminished the inhibitory effects of H2O2 on Klotho in NP cells. Consistently, Klotho knockdown by RNA interferences largely diminished the anti-inflammatory and intervertebral disc protective effects in an Intervertebral Disc Degeneration (IDD) model. Thus, our study indicates that TLR4-NF-κB signaling and Klotho form a negative-feedback loop in NP cells. Also, we demonstrate that the expression of Klotho is regulated by the balance between upregulation and downregulation of TLR4-NF-κB signaling.

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