scholarly journals Effect of autologous platelet-rich plasma-releasate on intervertebral disc degeneration in the rabbit anular puncture model: a preclinical study

2012 ◽  
Vol 14 (6) ◽  
pp. R241 ◽  
Author(s):  
Shuji Obata ◽  
Koji Akeda ◽  
Takao Imanishi ◽  
Koichi Masuda ◽  
Won Bae ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Platelet Rich Plasma ◽  
Preclinical Study ◽  
Autologous Platelet Rich Plasma ◽  
Puncture Model ◽  
Autologous Platelet

Autologous platelet-rich plasma (PRP) effect on intervertebral disc restoration: an experimental rabbit model

2018 ◽  
Vol 29 (3) ◽  
pp. 545-551 ◽  
Author(s):  
Ioannis D. Gelalis ◽  
Georgios Christoforou ◽  
Antonia Charchanti ◽  
Ioannis Gkiatas ◽  
Emilios Pakos ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Platelet Rich Plasma ◽  
Rabbit Model ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

scholarly journals Efficacy of Platelet-Rich Plasma Containing Xenogenic Adipose Tissue-Derived Stromal Cells on Restoring Intervertebral Disc Degeneration: A Preclinical Study in a Rabbit Model

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Chao Ma ◽  
Ran Wang ◽  
Dingliang Zhao ◽  
Naikun Wang ◽  
Ying Han ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Stromal Cells ◽  
Signal Intensity ◽  
Intervertebral Disc Degeneration ◽  
Platelet Rich Plasma ◽  
Rabbit Model ◽  
Control Group ◽  
Arterial Blood

Objective. Platelet-rich plasma (PRP) containing multiple growth factors is a promising strategy for disc degeneration. Thus, this study hypothesizes that the combination of PRP and adipose tissue-derived stromal cells (ADSCs) may repair degenerative disc more effectively than using each one of them alone. Methods. The model of early intervertebral disc degeneration was induced by annular puncture in the New Zealand rabbit. Autologous PRP was extracted from fresh arterial blood by using two centrifugation techniques. ADSC was offered by the Center for Clinic Stem Cell Research. Four weeks after the first experiment, PRP or ADSCs or a combination of PRP and ADSCs was injected into the punctured intervertebral disc. Four weeks later, disc height and signal intensity on T2-weighted magnetic resonance imaging (MRI) were assessed. Results. One month after puncture, we detected relatively narrow discs and lower signal intensity in MRI T2-weighted images. At four weeks after injection, the PRP-ADSC group statistically significantly restored discs, compared with PRP, ADSCs, or negative control group. Conclusions. The combination of PRP and ADSCs shows an effective potential to restore degenerated intervertebral discs in the rabbit.

scholarly journals The Differential Effects of Leukocyte-Containing and Pure Platelet-Rich Plasma on Nucleus Pulposus-Derived Mesenchymal Stem Cells: Implications for the Clinical Treatment of Intervertebral Disc Degeneration

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Jun Jia ◽  
Shan-zheng Wang ◽  
Liang-yu Ma ◽  
Jia-bin Yu ◽  
Yu-dong Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Platelet Rich Plasma ◽  
Enzyme Linked Immunosorbent Assay ◽  
Stem Cell Markers

Background. Platelet-rich plasma (PRP) is a promising strategy for intervertebral disc degeneration. However, the potential harmful effects of leukocytes in PRP on nucleus pulposus-derived mesenchymal stem cells (NPMSCs) have seldom been studied. This study aimed at comparatively evaluating effects of pure platelet-rich plasma (P-PRP) and leukocyte-containing platelet-rich plasma (L-PRP) on rabbit NPMSCs in vitro. Methods. NPMSCs isolated from rabbit NP tissues were treated with L-PRP or P-PRP in vitro, and then cell proliferation and expression of stem cell markers, proinflammatory cytokines (TNF-α, IL-1β), production of ECM (extracellular matrix-related protein), and NF-κB p65 protein were validated by CCK-8 assay, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, immunofluorescence, and western blot respectively. Results. NPMSCs differentiate into nucleus pulposus-like cells after treatment of PRPs (P-PRP and L-PRP), and NPMSCs exhibited maximum proliferation at a 10% PRP dose. L-PRP had observably higher concentration of leukocytes, TNF-α, and IL-1β than P-PRP. Furthermore, compared to P-PRP, L-PRP induced the differentiated NPMSCs to upregulate the expression of TNF-α and IL-1β, enhanced activation of the NF-κB pathway, increased the expression of MMP-1 and MMP-13, and produced less ECM in differentiated NPMSCs. Conclusions. Both P-PRP and L-PRP can induce the proliferation and NP-differentiation of NPMSCs. Compared to L-PRP, P-PRP can avoid the activation of the NF-κB pathway, thus reducing the inflammatory and catabolic responses.

scholarly journals Effect of small interference RNA (siRNA) for ADAMTS5 on intervertebral disc degeneration in the rabbit anular needle-puncture model

2009 ◽  
Vol 11 (6) ◽  
pp. R166 ◽  
Author(s):  
Shoji Seki ◽  
Yumiko Asanuma-Abe ◽  
Koichi Masuda ◽  
Yoshiharu Kawaguchi ◽  
Kunihiro Asanuma ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Needle Puncture ◽  
Small Interference Rna ◽  
Puncture Model ◽  
Interference Rna

Time course investigation of intervertebral disc degeneration in a rat-tail puncture model

Life Sciences ◽  
2016 ◽  
Vol 156 ◽  
pp. 15-20 ◽  
Author(s):  
Chia-Hsian Chen ◽  
Chang-Jung Chiang ◽  
Lien-Chen Wu ◽  
Chih-Hong Yang ◽  
Yi-Jie Kuo ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Time Course ◽  
Puncture Model ◽  
Rat Tail

P89. Oral administration of a prodrug of esculetin derivative inhibits intervertebral disc degeneration in the rabbit needle puncture model

2004 ◽  
Vol 4 (5) ◽  
pp. S102-S103
Author(s):  
Masahiko Okuma ◽  
Howard An ◽  
Koichi Nakagawa ◽  
Koji Akeda ◽  
Carol Muehleman ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Oral Administration ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Needle Puncture ◽  
Puncture Model
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