scholarly journals Single-Dose Local Simvastatin Injection Improves Implant Fixation via Increased Angiogenesis and Bone Formation in an Ovariectomized Rat Model

2015 ◽  
Vol 21 ◽  
pp. 1428-1439 ◽  
Author(s):  
Chunli Song
Keyword(s):  
Single Dose ◽  
Bone Formation ◽  
Rat Model ◽  
Ovariectomized Rat ◽  
Implant Fixation

Bone-selective analogs of human PTH(1-34) increase bone formation in an ovariectomized rat model

2009 ◽  
Vol 11 (5) ◽  
pp. 614-625 ◽  
Author(s):  
Nancy E. Lane ◽  
Donald B. Kimmel ◽  
Magnus H.L. Nilsson ◽  
Fred E. Cohen ◽  
Suellen Newton ◽  
...  
Keyword(s):  
Bone Formation ◽  
Rat Model ◽  
Ovariectomized Rat ◽  
Increase Bone Formation

Parathyroid hormone enhances osteogenesis in a rat model for extraosseous bone formation

1984 ◽  
Vol 104 (4_Supplb) ◽  
pp. S14 ◽  
Author(s):  
J. M. GOTTSWINTER ◽  
H. FLECHTNER ◽  
R. LÖWENSTEIN ◽  
R. ZIEGLER ◽  
G. DELLING
Keyword(s):  
Parathyroid Hormone ◽  
Bone Formation ◽  
Rat Model

P1237TISSUE-NONSPECIFIC ALKALINE PHOSPHATASE, A CULPRIT DURING ARTERIAL MEDIA CALCIFICATION BUT INDISPENSABLE DURING PHYSIOLOGICAL BONE FORMATION/-MINERALIZATION IN RATS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Britt Opdebeeck ◽  
José Millan Luis ◽  
Anthony Pinkerton ◽  
Anja Verhulst ◽  
Patrick D'Haese ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Formation ◽  
Vascular Calcification ◽  
Rat Model ◽  
Bone Mineralization ◽  
Calcium Content ◽  
Marker Genes ◽  
Peripheral Arteries ◽  
Calcium Phosphorus ◽  
Chondrogenic Marker

Abstract Background and Aims Vascular media calcification is frequently seen in elderly and patients with chronic kidney disease (CKD), diabetes and osteoporosis. Pyrophosphate is a well-known calcification inhibitor that binds to nascent hydroxyapatite crystals and prevents further incorporation of inorganic phosphate into these crystals. However, the enzyme tissue-nonspecific alkaline phosphatase (TNAP), which is highly expressed in calcified arteries, degrades extracellular pyrophosphate into phosphate ions, by which pyrophosphate loses its ability to block vascular calcification. Here, we aimed to evaluate whether a TNAP inhibitor is able to prevent the development of arterial calcification in a rat model of warfarin-induced vascular calcification. Method To induce vascular calcification, rats received a diet containing 0.30% warfarin and 0.15% vitamin K1 throughout the entire study and were subjected to the following daily treatments: (i) vehicle (n=10) or (ii) 10 mg/kg/day TNAP-inhibitor (n=10) administered via an intraperitoneal catheter from start of the study until sacrifice at week 7. Calcium, phosphorus and parathyroid hormone (PTH) levels were determined in serum samples as these are important determinants of vascular calcification. As TNAP is also expressed in the liver, serum alanine aminotransferase (ALT) and aspartate (AST) levels were analyzed. At sacrifice, vascular calcification was evaluated by measurement of the total calcium content in the arteries and quantification of the area % calcification on Von Kossa stained sections of the aorta. The mRNA expression of osteo/chondrogenic marker genes (runx2, TNAP, SOX9, collagen 1 and collagen 2) was analyzed in the aorta by qPCR to verify whether vascular smooth muscle cells underwent reprogramming towards bone-like cells. Bone histomorphometry was performed on the left tibia to measure static and dynamic bone parameters as TNAP also regulates physiological bone mineralization. Results No differences in serum calcium, phosphorus and PTH levels was observed between both study groups. Warfarin exposure resulted in distinct calcification in the aorta and peripheral arteries. Daily dosing with the TNAP inhibitor (10 mg/kg/day) for 7 weeks significantly reduced vascular calcification as indicated by a significant decrease in calcium content in the aorta (vehicle 3.84±0.64 mg calcium/g wet tissue vs TNAP inhibitor 0.70±0.23 mg calcium/g wet tissue) and peripheral arteries and a distinct reduction in area % calcification on Von Kossa stained aortic sections as compared to vehicle condition. The inhibitory effects of SBI-425 on vascular calcification were without altering serum liver markers ALT and AST levels. Furthermore, TNAP-inhibitor SBI-425 did not modulate the mRNA expression of osteo/chondrogenic marker genes runx2, TNAP, SOX9, collagen 1 and 2. Dosing with SBI-425 resulted in decreased bone formation rate and mineral apposition rate, and increased osteoid maturation time and this without significant changes in osteoclast- and eroded perimeter. Conclusion Dosing with TNAP inhibitor SBI-425 significantly reduced the calcification in the aorta and peripheral arteries of a rat model of warfarin-induced vascular calcification and this without affecting liver function. However, suppression of TNAP activity should be limited in order to maintain adequate physiological bone mineralization.

scholarly journals Sclerostin Antibody Treatment Increases Bone Formation, Bone Mass, and Bone Strength in a Rat Model of Postmenopausal Osteoporosis*

2009 ◽  
Vol 24 (4) ◽  
pp. 578-588 ◽  
Author(s):  
Xiaodong Li ◽  
Michael S Ominsky ◽  
Kelly S Warmington ◽  
Sean Morony ◽  
Jianhua Gong ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Mass ◽  
Postmenopausal Osteoporosis ◽  
Bone Strength ◽  
Rat Model ◽  
Antibody Treatment ◽  
Sclerostin Antibody

Progressive Loss of Implant Fixation in a Preclinical Rat Model of Cemented Knee Arthroplasty

2020 ◽  
Author(s):  
Kenneth A Mann ◽  
Mark A Miller ◽  
Jeffrey K Rossow ◽  
Megan E Tatusko ◽  
Jason A Horton ◽  
...  
Keyword(s):  
Knee Arthroplasty ◽  
Rat Model ◽  
Implant Fixation ◽  
Progressive Loss

Effect of Intermittent and Daily Regimens of Minodronic Acid on Bone Metabolism in an Ovariectomized Rat Model of Osteoporosis

2014 ◽  
Vol 95 (2) ◽  
pp. 166-173 ◽  
Author(s):  
Makoto Tanaka ◽  
Hiroshi Mori ◽  
Ryoji Kayasuga ◽  
Yasuo Ochi ◽  
Hiroyuki Yamada ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Rat Model ◽  
Ovariectomized Rat ◽  
Minodronic Acid
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