scholarly journals Emerging applications in mass spectrometry imaging; enablers and roadblocks

2020 ◽  
Author(s):  
Cristina Russo ◽  
Cameron Heaton ◽  
Lucy Flint ◽  
Oana Voloaca ◽  
Sarah Haywood-Small ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Spatial Distribution ◽  
Mass Spectrometry Imaging ◽  
Clinical Applications ◽  
Widespread Application ◽  
Wide Range ◽  
Extensive Range ◽  
Technical Advances ◽  
Versatile Technique ◽  
Clinical Drug

Mass spectrometry imaging (MSI) is a powerful and versatile technique able to investigate the spatial distribution of multiple non-labelled endogenous and exogenous analytes simultaneously, within a wide range of samples. Over the last two decades, MSI has found widespread application for an extensive range of disciplines including pre-clinical drug discovery, clinical applications and human identification for forensic purposes. Technical advances in both instrumentation and software capabilities have led to a continual increase in the interest in MSI; however, there are still some limitations. In this review, we discuss the emerging applications in MSI that significantly impact three key areas of mass spectrometry (MS) research—clinical, pre-clinical and forensics—and roadblocks to the expansion of use of MSI in these areas.

scholarly journals Unveiling the spatial distribution of aflatoxin B1 and plant defense metabolites in maize using AP‐SMALDI mass spectrometry imaging

2021 ◽  
Author(s):  
Laura Righetti ◽  
Dhaka Ram Bhandari ◽  
Enrico Rolli ◽  
Sara Tortorella ◽  
Renato Bruni ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Spatial Distribution ◽  
Plant Defense ◽  
Aflatoxin B1 ◽  
Mass Spectrometry Imaging

Cell and Tissue Imaging by TOF-SIMS and MALDI-TOF: An Overview for Biological and Pharmaceutical Analysis

2021 ◽  
pp. 1-26
Author(s):  
Manale Noun ◽  
Rayane Akoumeh ◽  
Imane Abbas
Keyword(s):  
Mass Spectrometry ◽  
Spatial Distribution ◽  
Time Of Flight ◽  
Molecular Ions ◽  
Tissue Imaging ◽  
Diagnostic Tools ◽  
Good Precision ◽  
Maldi Tof ◽  
Tof Sims ◽  
Wide Range

Abstract The potential of mass spectrometry imaging (MSI) has been demonstrated in cell and tissue research since 1970. MSI can reveal the spatial distribution of a wide range of atomic and molecular ions detected from biological sample surfaces, it is a powerful and valuable technique used to monitor and detect diverse chemical and biological compounds, such as drugs, lipids, proteins, and DNA. MSI techniques, notably matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) and time of flight secondary ion mass spectrometry (TOF-SIMS), witnessed a dramatic upsurge in studying and investigating biological samples especially, cells and tissue sections. This advancement is attributed to the submicron lateral resolution, the high sensitivity, the good precision, and the accurate chemical specificity, which make these techniques suitable for decoding and understanding complex mechanisms of certain diseases, as well as monitoring the spatial distribution of specific elements, and compounds. While the application of both techniques for the analysis of cells and tissues is thoroughly discussed, a briefing of MALDI-TOF and TOF-SIMS basis and the adequate sampling before analysis are briefly covered. The importance of MALDI-TOF and TOF-SIMS as diagnostic tools and robust analytical techniques in the medicinal, pharmaceutical, and toxicology fields is highlighted through representative published studies.

scholarly journals Localization of Flavan-3-ol Species in Peanut Testa by Mass Spectrometry Imaging

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2373 ◽  
Author(s):  
Hirofumi Enomoto ◽  
Takashi Nirasawa
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Region Of Interest ◽  
Heterocyclic Ring ◽  
Biological Properties ◽  
Quinone Methide ◽  
Negative Ion ◽  
Ionization Mass ◽  
Wide Range ◽  
Outer Epidermis

Flavan-3-ols, procyanidins and their monomers are major flavonoids present in peanuts that show a wide range of biological properties and health benefits, based on their potent antioxidant activity. Procyanidin oligomers, especially A-type, are reportedly abundant in peanut skin; however, their localization in the raw peanut testa remains poorly understood. Therefore, we performed matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to investigate the localization of flavan-3-ols in peanut testa. 1,5-Diaminonaphthalene was coated onto the peanut section by matrix vapor deposition/recrystallization, and MALDI-MSI measurements were performed in the negative-ion mode. Peaks matching the m/z values of flavan-3-ol [M − H]− ions were observed in the mass spectrum extracted from the outer epidermis of the peanut testa, using the region of interest function. Catechin and/or epicatechin, five A-type, and one B-type procyanidins were assigned by the fragment ions generated by retro-Diels-Alder, heterocyclic ring fission, and quinone methide reactions detected in MALDI-tandem MS spectra. These flavan-3-ols were localized in the outer epidermis of the peanut testa. This information will contribute to improving the extraction and purification efficiencies of flavan-3-ols from peanut testa. As flavan-3-ols display anti-microbial activity, it is speculated that flavan-3-ols present in the outer epidermis of peanut testa act to prevent pathogen infection.

scholarly journals Spermidine and other functional phytochemicals in soybean seeds: Spatial distribution as visualized by mass spectrometry imaging

2019 ◽  
Vol 8 (1) ◽  
pp. 675-682 ◽  
Author(s):  
Tatsuya Sagara ◽  
Dhaka Ram Bhandari ◽  
Bernhard Spengler ◽  
Johann Vollmann
Keyword(s):  
Mass Spectrometry ◽  
Spatial Distribution ◽  
Mass Spectrometry Imaging ◽  
Soybean Seeds

scholarly journals Mass spectrometry imaging: linking molecule profiles to tissue spatial distribution

2013 ◽  
Vol 10 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Hassan Dihazi ◽  
Rainer Bohrer ◽  
Olaf Jahn ◽  
Christof Lenz ◽  
Andrzej Majcherczyk ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Spatial Distribution ◽  
Mass Spectrometry Imaging

scholarly journals Mass spectrometry imaging and monitoring of in vivo glutathione-triggered cisplatin release from nanoparticles in the kidneys

2020 ◽  
Vol 2 (12) ◽  
pp. 5857-5865
Author(s):  
Arthur C. K. Chung ◽  
Xuan Li ◽  
Wai-Chung Li ◽  
Tao Wang ◽  
Hin-Kiu Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Broad Spectrum ◽  
Mass Spectrometry Imaging ◽  
Clinical Applications ◽  
Chemotherapeutic Drug

An increasing number of studies have reported the use of various nanoparticles to encapsulate cisplatin, a frontline chemotherapeutic drug against a broad-spectrum of cancers, for overcoming its inherent drawbacks in clinical applications.

scholarly journals High resolution mass spectrometry imaging of plant tissues: towards a plant metabolite atlas

The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7696-7709 ◽  
Author(s):  
Dhaka Ram Bhandari ◽  
Qing Wang ◽  
Wolfgang Friedt ◽  
Bernhard Spengler ◽  
Sven Gottwald ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
High Spatial Resolution ◽  
High Resolution Mass Spectrometry ◽  
Chemical Imaging ◽  
Plant Tissues ◽  
Label Free ◽  
Wide Range ◽  
Plant Metabolite ◽  
Resolution Mass

Label-free chemical imaging of a wide range of metabolites in all major plant organs acquired at high spatial resolution.

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