Localization of gelatinase activities in glioma tissues by film in situ zymography

2001 ◽  
Vol 18 (2) ◽  
pp. 145-150 ◽  
Author(s):  
Shingo Takano ◽  
Koji Tsuboi ◽  
Akira Matsumura ◽  
Hiroshige Sato ◽  
Tadao Nose
Keyword(s):  
Film In Situ Zymography ◽  
In Situ Zymography

Evaluation of matrix metalloproteinase-2 (mmp-2) activity with film in situ zymography for improved cytological diagnosis of Breast tumors

Breast Cancer ◽  
2006 ◽  
Vol 13 (3) ◽  
pp. 272-278 ◽  
Author(s):  
Akira Fujiwara ◽  
Eri Shibata ◽  
Hitomi Terashima ◽  
Akemi Shishido ◽  
Junko Nishiki ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Breast Tumors ◽  
Matrix Metalloproteinase 2 ◽  
Cytological Diagnosis ◽  
Film In Situ Zymography ◽  
In Situ Zymography

In Vivo Localization of Gelatinases (MMP-2 and -9) by in Situ Zymography with a Selective Gelatinase Inhibitor

2001 ◽  
Vol 287 (3) ◽  
pp. 766-774 ◽  
Author(s):  
Emma Pirilä ◽  
Päivi Maisi ◽  
Tuula Salo ◽  
Erkki Koivunen ◽  
Timo Sorsa
Keyword(s):  
In Situ Zymography

Simultaneous enzyme overlay membrane (EOM)-based in situ zymography and immunofluorescence technique reveals cathepsin B-like activity in a subset of tumour vessels

2000 ◽  
Vol 114 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Alexander Berndt ◽  
Jördis Johannesson ◽  
Michael Haas ◽  
Christoph Arkona ◽  
Detlef Katenkamp ◽  
...  
Keyword(s):  
Cathepsin B ◽  
Immunofluorescence Technique ◽  
In Situ Zymography

Abstract 212: Fractalkine Neutralization Improves Cardiac Function After Myocardial Infarction

Hypertension ◽  
2014 ◽  
Vol 64 (suppl_1) ◽  
Author(s):  
Xiaosong Gu ◽  
Jiang Xu ◽  
Xiao-Ping Yang ◽  
Edward Peterson ◽  
Pamela Harding
Keyword(s):  
Myocardial Infarction ◽  
Cardiac Function ◽  
Macrophage Infiltration ◽  
Receptor Subtype ◽  
Cross Sectional ◽  
Gelatinase Activity ◽  
Ep4 Receptor ◽  
In Situ Zymography ◽  
Circulating Levels

Background: Circulating levels of the chemokine fractalkine (FKN) are increased in patients with chronic heart failure (HF) and our studies show that aged mice lacking the prostaglandin E2 EP4 receptor subtype (EP4 KO) have increased cardiac FKN coupled with a phenotype of dilated cardiomyopathy. However, whether FKN is a causal factor for HF is not well established. Hypothesis: FKN contributes to the pathogenesis of HF post myocardial infarction (MI) and EP4 KO mice have a better response to anti-FKN treatment due to elevated FKN levels. Methods: Male EP4 KO mice and wild type (WT) littermates underwent surgery to induce MI. Mice were treated with an anti-FKN antibody (40μg/kg/day, ip) or IgG immediately after MI and echocardiography was performed 2 wks post MI. Hearts were excised for infarct size determination, myocyte cross-sectional area (MCSA) and interstitial collagen fraction (ICF) determined by morphometric analysis and macrophage infiltration using immunohistochemistry. Results: Anti-FKN treatment improved cardiac function and prevented remodeling (Table). In situ zymography revealed that gelatinase activity was markedly reduced by anti-FKN treatment in both strains. Moreover, anti-FKN treatment tended to improve survival in EP4 KO mice (p = 0.06, n=20). Conclusions: (1) FKN contributes to the pathogenesis of HF and anti-FKN treatment improves cardiac function and reduces cardiac remodeling. This may be related to reduced macrophage infiltration and decreased gelatinase activity.(2) Contrary to our hypothesis, EP4 KO mice do not have an enhanced response to anti-FKN treatment.

scholarly journals MMP Activity in the Hybrid Layer Detected with in situ Zymography

2012 ◽  
Vol 91 (5) ◽  
pp. 467-472 ◽  
Author(s):  
A. Mazzoni ◽  
F.D. Nascimento ◽  
M. Carrilho ◽  
I. Tersariol ◽  
V. Papa ◽  
...  
Keyword(s):  
Hybrid Layer ◽  
In Situ Zymography
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