Fungal Metabolites. XV. Primary Structures of Antibiotic Peptides, Hypelcins B-I, B-II, B-III, B-IV and B-V, from Hypocrea peltata. Application of Electrospray Mass Spectrometry and Electrospray Mass Spectrometry/Mass Spectrometry.

1994 ◽  
Vol 42 (5) ◽  
pp. 1063-1069 ◽  
Author(s):  
Kouji MATSUURA ◽  
Osamu SHIMA ◽  
Yoshio TAKEDA ◽  
Yoshihisa TAKAISHI ◽  
Yasuo NAGAOKA ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Mass Spectrometry ◽  
Fungal Metabolites ◽  
Primary Structures ◽  
Mass Spectrometry Mass Spectrometry ◽  
Antibiotic Peptides

Proteomic Detection of Beta Thalassaemia Trait Using Electrospray Mass Spectrometry-Mass Spectrometry.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3823-3823
Author(s):  
Yvonne Daniel ◽  
Charles Turner ◽  
R. Neil Dalton
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Mass Spectrometry ◽  
Multiple Reaction Monitoring ◽  
Simultaneous Detection ◽  
Average Mass ◽  
Beta Thalassaemia ◽  
Thalassaemia Trait ◽  
Arginine Residues ◽  
Clinically Significant ◽  
Mass Spectrometry Mass Spectrometry

Abstract Introduction Mass spectrometry has proved a valuable tool in the unequivocal characterisation of haemoglobin variants detected during routine haemoglobinopathy screening using High Performance Liquid Chromatography (HPLC) and/or isoelectric focusing. We have recently described a rapid and specific electrospray mass spectrometry-mass spectrometry (MSMS) method, using proteomic mutation targeting, for simultaneous detection of the clinically significant haemoglobinopathies; haemoglobin (Hb)S, HbC, HbE, HbDPunjab and HbOArab. Here we report additional transitions within the 1min cycle time, using delta chains as a surrogate HbA2, with the objective of detecting beta thalassaemia trait. Method Blood samples are digested using trypsin, which results in specific cleavage of globin chains at peptide bonds adjacent to lysine and arginine residues, to produce a series of well-characterised peptides. There is strong amino acid sequence homology between the beta and delta chains of haemoglobin, however tryptic digestion produces series of 15 (T1–T15) and 16 (T1–T16) peptides, for beta and delta chains respectively, with differences in T2, T3, T5, T10, T12, T13 and T14. In this report we have investigated the diagnostic utility of T2: beta sequence SAVTALWGK, average mass 932.1 daltons, delta sequence TAVNALWGK, average mass 959.1 daltons. In multiple reaction monitoring mode the respective T2 [M+2H]2+ ions, m/z 466.8 and 480.3, are selected, fragmented, and the singly charged ions of the informative peptide fragments, VTALWGK and VNALWGK, detected. Results The percentage area ratio of delta/(beta+delta) was calculated and compared with the classical percentage HbA2 value obtained by HPLC. 66 samples with HbA2 values within our normal range (mean 2.7%, range 1.8–3.4), and 58 with HbA2 values indicative of beta thalassaemia trait (5.2%, 4.2–7.9) were analysed by MSMS and the corresponding delta/(beta+delta) ratios were 1.7%, 0.9–2.3 and 3.4%, 2.5–6.0. In addition, in 2 further HbS beta thalassaemia zero compound heterozygote samples confirmed by beta gene sequencing the ratios were 4.1% and 3.7%. Conclusion This relatively small study provides evidence that beta thalassaemia trait may be diagnosed by MSMS and represents another step towards comprehensive MSMS population screening for clinically significant haemoglobinopathies.

New caerin antibiotic peptides from the skin secretion of the Dainty Green Tree Frog Litoria gracilenta. Identification using positive and negative ion electrospray mass spectrometry

Toxicon ◽  
2006 ◽  
Vol 47 (6) ◽  
pp. 664-675 ◽  
Author(s):  
Micheal J. Maclean ◽  
Craig S. Brinkworth ◽  
Daniel Bilusich ◽  
John H. Bowie ◽  
Jason R. Doyle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Mass Spectrometry ◽  
Negative Ion ◽  
Tree Frog ◽  
Skin Secretion ◽  
Antibiotic Peptides
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