Fungal metabolites. Part 5. Rapid structure elucidation of antibiotic peptides, minor components of Trichosporin Bs from Trichoderma polysporum. Application of linked-scan and continuous-flow fast-atom bombardment mass spectrometry

1993 ◽  
pp. 357 ◽  
Author(s):  
Junko Iida ◽  
Akira Iida ◽  
Yoshiko Takahashi ◽  
Yoshihisa Takaishi ◽  
Yasuo Nagaoka ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Continuous Flow ◽  
Structure Elucidation ◽  
Fast Atom Bombardment ◽  
Minor Components ◽  
Fungal Metabolites ◽  
Antibiotic Peptides

Coaxial continuous flow fast atom bombardment in conjunction with tandem mass spectrometry for the analysis of biomolecules

1989 ◽  
Vol 61 (22) ◽  
pp. 2504-2511 ◽  
Author(s):  
Leesa J. Deterding ◽  
M. Arthur. Moseley ◽  
Kenneth B. Tomer ◽  
James W. Jorgenson
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Continuous Flow ◽  
Fast Atom Bombardment ◽  
Tandem Mass

Continuous flow fast atom bombardment mass spectrometry of mononucleotides and their metal complexes

1989 ◽  
Vol 67 (5) ◽  
pp. 910-920 ◽  
Author(s):  
M. J. Bertrand ◽  
V. Benham ◽  
R. St-Louis ◽  
M. J. Evans
Keyword(s):  
Mass Spectrometry ◽  
Continuous Flow ◽  
Fast Atom Bombardment ◽  
Aqueous Media ◽  
Molecular Ions ◽  
Fragment Ions ◽  
Low Concentrations ◽  
Metal Adducts ◽  
Matrix Concentration

The mass spectra of mononucleotides and their metal adducts Na, K, Mg, Ca, Ni, Co, Cu, and Zn of guanosine 5′-monophosphate (5′-GMP) as well as H, Na, and Mg of adenosine 5′-monophosphate (5′-AMP) and H and Ni of inosine 5′-monophosphate (5′-IMP) have been obtained in low concentrations of matrix in water using continuous-flow fast atom bombardment. The results indicate that this technique is suitable for the analysis of these complexes in aqueous media and yields spectra that are highly characteristic of the compounds analyzed. Parent-molecular ions and structurally significant fragment ions are observed for all compounds studied and the different binding sites for the metal on the nucleotides can be isolated from the fragment ions. Experimental parameters influencing the quality of the spectra such as flow rate, matrix concentration, matrix nature, and analyte concentration have been studied and optimized. For the thirteen compounds studied, it appears that continuous-flow FAB is superior to conventional FAB and that good quality spectra can be obtained with as little as 0.5% of added matrix thus minimizing spectral interferences. Keywords: continuous flow FAB, FAB MS, mass spectrometry, nucleotides, metal-nucleotides.

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