scholarly journals Effect of potassium ions on insulin release in the rat from the perifused islets of Langerhans with slow-rise glucose stimulation.

1979 ◽  
Vol 27 (9) ◽  
pp. 2198-2202 ◽  
Author(s):  
KUNIO KOBAYASHI ◽  
SHOHEI KAGAWA ◽  
YOKO TSUMURA ◽  
HISAYO ISE ◽  
KEIKO YOSHIDA ◽  
...  
Keyword(s):  
Insulin Release ◽  
Islets Of Langerhans ◽  
Potassium Ions ◽  
Glucose Stimulation ◽  
Slow Rise ◽  
Perifused Islets

scholarly journals Potassium ions and the secretion of insulin by islets of Langerhans incubated in vitro

1968 ◽  
Vol 108 (1) ◽  
pp. 17-24 ◽  
Author(s):  
S. L. Howell ◽  
K W Taylor
Keyword(s):  
Insulin Secretion ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Potassium Ions ◽  
Collagenase Digestion ◽  
Islet Tissue ◽  
Rabbit Pancreas ◽  
K Concentration

1. A method was devised for the isolation of islets of Langerhans from rabbit pancreas by collagenase digestion in order to study the influx and efflux of K+ in islets during insulin secretion. 2. Glucose-induced insulin release was accompanied by an increased rate of uptake of 42K+ by the islets of Langerhans, though this was not the case for secretion in response to tolbutamide. Ouabain significantly inhibited the uptake of 42K+ by islet tissue. 3. No significant increase in the rate of efflux of 42K+ was demonstrated during active insulin secretion. 4. Slices of rabbit pancreas were incubated in media of different K+ content, and rates of insulin release were determined. Alteration of the K+ concentration of the medium between 3 and 8mm had no effect on the rate of insulin release by pancreas slices. However, decrease of the K+ concentration to 1mm resulted in inhibition of secretion in response to both glucose and to tolbutamide. Conversely, an increase in K+ concentration increased rates of insulin release in response to both these stimuli. 5. It is concluded that, though unphysiological concentrations of K+ may influence the secretion of insulin, fluxes of K+ in the islets do not appear to be important in the initiation of insulin secretion.

scholarly journals Insulin release from the perifused isolated rat langerhans islets under a slow-rise glucose stimulation.

1977 ◽  
Vol 25 (8) ◽  
pp. 2112-2116 ◽  
Author(s):  
KUNIO KOBAYASHI ◽  
YOKO TSUMURA ◽  
HISAYO ISE ◽  
SHOHEI KAGAWA ◽  
AKIRA MATSUOKA
Keyword(s):  
Insulin Release ◽  
Glucose Stimulation ◽  
Langerhans Islets ◽  
Slow Rise ◽  
Isolated Rat

INSULIN COMPONENTS RELEASED BY THE NORMAL AND SUBTOTAL DEGRANULATED PERIFUSED ISLETS OF LANGERHANS OF THE RAT

1975 ◽  
Vol 78 (3) ◽  
pp. 524-530 ◽  
Author(s):  
O. García Hermida ◽  
J. Gómez-Acebo
Keyword(s):  
Insulin Release ◽  
Islets Of Langerhans ◽  
The Other ◽  
Second Phase ◽  
Other Hand ◽  
Perifused Islets ◽  
Main Component ◽  
Perifusion System

ABSTRACT The insulin components released from normal and tolbutamide treated islets of the rat were studied in a perifusion system. The treated islets showed diminished quantities of insulin secreted as compared to the controls and an increase in the proportions of "big insulin" secreted during the second phase of this release. On the other hand, the main component of insulin released during the first phase was "little insulin". These studies confirm that the subtotally degranulated islets are less efficient than the controls in producing insulin release.

Sensitized effect of prestimulation on insulin release from the perifused rat islets with a slow-rise glucose stimulation.

1977 ◽  
Vol 24 (5) ◽  
pp. 451-456
Author(s):  
KUNIO KOBAYASHI ◽  
YOKO TSUMURA ◽  
HISAYO ISE ◽  
SHOHEI KAGAWA ◽  
AKIRA MATSUOKA
Keyword(s):  
Insulin Release ◽  
Glucose Stimulation ◽  
Rat Islets ◽  
Slow Rise

Effect of fasting on the release of insulin and somatostatin from perifused islets of Langerhans

Diabetes ◽  
1979 ◽  
Vol 28 (3) ◽  
pp. 204-207 ◽  
Author(s):  
P. Schauder ◽  
C. McIntosh ◽  
J. Arends ◽  
H. Frerichs
Keyword(s):  
Islets Of Langerhans ◽  
Perifused Islets

scholarly journals Nitric oxide inhibits, and carbon monoxide activates, islet acid α-glucoside hydrolase activities in parallel with glucose-stimulated insulin secretion

2006 ◽  
Vol 190 (3) ◽  
pp. 681-693 ◽  
Author(s):  
Henrik Mosén ◽  
Albert Salehi ◽  
Ragnar Henningsson ◽  
Ingmar Lundquist
Keyword(s):  
Nitric Oxide ◽  
Carbon Monoxide ◽  
Insulin Secretion ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Direct Effect ◽  
Appreciable Effect ◽  
Intact Mouse ◽  
Glucose Stimulated Insulin Secretion ◽  
Glucoside Hydrolases

We have studied the influence of nitric oxide (NO) and carbon monoxide (CO), putative messenger molecules in the brain as well as in the islets of Langerhans, on glucose-stimulated insulin secretion and on the activities of the acid α-glucoside hydrolases, enzymes which we previously have shown to be implicated in the insulin release process. We have shown here that exogenous NO gas inhibits, while CO gas amplifies glucose-stimulated insulin secretion in intact mouse islets concomitant with a marked inhibition (NO) and a marked activation (CO) of the activities of the lysosomal/vacuolar enzymes acid glucan-1,4-α-glucosidase and acid α-glucosidase (acid α-glucoside hydrolases). Furthermore, CO dose-dependently potentiated glucose-stimulated insulin secretion in the range 0.1–1000 μM. In intact islets, the heme oxygenase substrate hemin markedly amplified glucose-stimulated insulin release, an effect which was accompanied by an increased activity of the acid α-glucoside hydrolases. These effects were partially suppressed by the guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one. Hemin also inhibited inducible NO synthase (iNOS)-derived NO production probably through a direct effect of CO on the NOS enzyme. Further, exogenous CO raised the content of both cGMP and cAMP in parallel with a marked amplification of glucose-stimulated insulin release, while exogenous NO suppressed insulin release and cAMP, leaving cGMP unaffected. Emiglitate, a selective inhibitor of α-glucoside hydrolase activities, was able to markedly inhibit the stimulatory effect of exogenous CO on both glucose-stimulated insulin secretion and the activityof acid glucan-1,4-α-glucosidase and acid α-glucosidase, while no appreciable effect on the activities of other lysosomal enzyme activities measured was found. We propose that CO and NO, both produced in significant quantities in the islets of Langerhans, have interacting regulatory roles on glucose-stimulated insulin secretion. This regulation is, at least in part, transduced through the activity of cGMP and the lysosomal/vacuolar system and the associated acid α-glucoside hydrolases, but probably also through a direct effect on the cAMP system.

Insulin release from isolated islets of Langerhans of the rat in organ culture

1973 ◽  
Vol 29 (7) ◽  
pp. 881-882 ◽  
Author(s):  
Brigitte Ziegler ◽  
R. Butter ◽  
H. -J. Hahn ◽  
R. Mehling ◽  
H. Fiedler
Keyword(s):  
Organ Culture ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Isolated Islets ◽  
Isolated Islets Of Langerhans
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