Insulin release from isolated islets of Langerhans of the rat in organ culture

1973 ◽  
Vol 29 (7) ◽  
pp. 881-882 ◽  
Author(s):  
Brigitte Ziegler ◽  
R. Butter ◽  
H. -J. Hahn ◽  
R. Mehling ◽  
H. Fiedler
Keyword(s):  
Organ Culture ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Isolated Islets ◽  
Isolated Islets Of Langerhans

scholarly journals A species difference in nucleoside phosphorylase activity and inosine-stimulated insulin secretion in isolated islets of Langerhans

1977 ◽  
Vol 168 (3) ◽  
pp. 591-593 ◽  
Author(s):  
I L Campbell ◽  
K W Taylor
Keyword(s):  
Insulin Secretion ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Species Differences ◽  
Species Difference ◽  
Isolated Islets ◽  
Phosphorylase Activity ◽  
Nucleoside Phosphorylase ◽  
Isolated Islets Of Langerhans

Inosine is a potent simulant of insulin release from rat but not from rabbit islets of Langerhans. Further investigation showed that nucleoside phosphorylase activity is exceptionally low in rabbit islets. The ability of inosine to promote insulin release seems to be related to islet nucleoside phosphorylase activity, which can display marked species differences.

scholarly journals Identification and metabolism of polyphosphoinositides in isolated islets of Langerhans

1983 ◽  
Vol 216 (1) ◽  
pp. 101-106 ◽  
Author(s):  
S G Laychock
Keyword(s):  
Insulin Release ◽  
Islets Of Langerhans ◽  
Isolated Islets ◽  
Half Time ◽  
Glucose Effect ◽  
Isolated Islets Of Langerhans ◽  
Metabolic Event ◽  
Phosphatidylinositol 4

Isolated islets were incubated with [32P]P1 and radiolabelling of polyphosphoinositides were determined. Labelling equilibrium was approached after 45 min, with a half-time of 15 min. D-Glucose decreased the amount of [32P]PO4 in phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] and phosphatidylinositol 4-phosphate (PtdIns4P) within 0.5 min, and loss of radiolabel was still evident at 1 min. [32P]PO4 levels in polyphosphoinositides returned to basal levels within 5 min. Neither D-galactose nor D-glucose after pretreatment of islets with mannoheptulose elicited the polyphosphoinositide effect. The glucose-stimulated breakdown of polyphosphoinositides was inhibited by EGTA; re-addition of Ca2+ partially restored the glucose effect. Ionomycin and tolbutamide promoted the rapid breakdown of PtdIns(4,5)P2, whereas the breakdown of PtdIns4P was less rapid and of a lesser magnitude. The results suggest that the Ca2+-dependent breakdown of polyphosphoinositides in an early metabolic event during the initiation of insulin release.

Insulin Release in Aging: Studies on Adenylate Cyclase, Phosphodiesterase, and Protein Kinase in Isolated Islets of Langerhans of the Rat*

Endocrinology ◽  
1981 ◽  
Vol 108 (2) ◽  
pp. 620-624 ◽  
Author(s):  
LOREN G. LIPSON ◽  
VICTORIA A. LIPSON ◽  
MICHAEL J. BUSH ◽  
GRETCHEN E. TIETJEN ◽  
AEREE YOON
Keyword(s):  
Protein Kinase ◽  
Adenylate Cyclase ◽  
Insulin Release ◽  
Islets Of Langerhans ◽  
Isolated Islets ◽  
Isolated Islets Of Langerhans ◽  
Aging Studies
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