scholarly journals Antitumor Activity of Bacillus natto. V. Isolation and Characterization of Surfactin in the Culture Medium of Bacillus natto KMD 2311

1974 ◽  
Vol 22 (4) ◽  
pp. 938-944 ◽  
Author(s):  
YUKIO KAMEDA ◽  
SADAO OUHIRA ◽  
KATSUHIKO MATSUI ◽  
SHOICHI KANATOMO ◽  
TETSU HASE ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Culture Medium ◽  
Bacillus Natto ◽  
Isolation And Characterization

Isolation and characterization of the ethynylestradiol-biodegrading microorganism Fusarium proliferatum strain HNS-1

2002 ◽  
Vol 45 (12) ◽  
pp. 175-179 ◽  
Author(s):  
J.H. Shi ◽  
Y. Suzuki ◽  
B.-D. Lee ◽  
S. Nakai ◽  
M. Hosomi
Keyword(s):  
Culture Medium ◽  
Polar Compounds ◽  
Sole Source ◽  
Order Rate Constant ◽  
Fusarium Proliferatum ◽  
Rrna Gene ◽  
28S Rrna ◽  
Isolation And Characterization ◽  
Phenolic Group

We cultivated hundreds of sediment, soil, and manure samples taken from rivers and farms in a medium containing ethynylestradiol (EE2) as the sole source of carbon, so that microorganisms in the samples would acclimatize to the presence of EE2. Finally, we isolated an EE2-degrading microorganism, designated as strain HNS-1, from a cowshed sample. Based on its partial nucleotide sequence (563 bp) of the 28S rRNA gene, strain HNS-1 was identified as Fusarium proliferatum. Over 15 days, F. proliferatum strain HNS-1 removed 97% of EE2 at an initial concentration of 25 mg.L−1, with a first-order rate constant of 0.6 d−1. Unknown products of EE2 degradation, which may be more polar compounds that have a phenolic group, remained in the culture medium.

Isolation and characterization of bacterial diversity from soils supplemented with electrical transformer fluids

2011 ◽  
Vol 1 (2) ◽  
pp. 107-113
Author(s):  
Nwinyi Obinna C ◽  
Alade Adetutu ◽  
Leo‐ Akpan Imaobong R ◽  
Oladele Bolaji.O
Keyword(s):  
Optical Density ◽  
Culture Medium ◽  
Agricultural Land ◽  
Agricultural Soils ◽  
Bacterial Species ◽  
Minimal Salt Medium ◽  
Isolation And Characterization ◽  
The Mean ◽  
Biomass Increase

Repetitive enrichment of soils samples from an agricultural land and newly marked dumpsite on electrical transformer fluid yielded six bacterial species that have the capacity to utilize electrical transformer fluids (askarel) as carbon and energy source. Bacterial species namely: Micrococcus, Arthrobacter, Pseudomonas putida, Pseudomonas spp, Norcadia and Corynebacterium were identified using morphological and biochemical characteristics. The abilities of these bacterial species to utilize the electrical transformer fluids as carbon source in minimal salt medium (MSM); sub cultured in concentrations of 5, 10, 15 and 20μL of electrical transformer fluids were investigated for twenty‐one days period. Physiological changes in terms of biomass increase were monitored by measuring the pH and optical density of the culture medium. From the results obtained, there was observed a general decrease in the pH and  increase in Optical density (O.D). The mean pH and O.D. readings ranged between (4.34‐6.13 and 0.073‐0.386) respectively. The decreased pH could justify for the acidic metabolites produced in the course of utilization of askarel as growth substrates. This study suggested that, the tropical ecosystems can provide exotic bacterial species that are capable of degrading or mineralizing polychlorinated biphenyls and their derivatives from dumpsites and agricultural soils.

Isolation and Characterization of Viridin, a New 65 kDa Antifungal Protein from the Mould Trichoderma viride

1999 ◽  
Vol 380 (10) ◽  
pp. 1243-1245 ◽  
Author(s):  
Jian-Jiang Hao ◽  
Chuan-dong Geng ◽  
Wei-jun Xie ◽  
Zhen-zhen Gong ◽  
Wang-Yi Liu ◽  
...  
Keyword(s):  
High Performance ◽  
Culture Medium ◽  
Basic Protein ◽  
Trichoderma Viride ◽  
Exchange Chromatography ◽  
Antifungal Protein ◽  
Cation Exchange Chromatography ◽  
Isolation And Characterization ◽  
Sds Page

AbstractA new extracellular antifungal protein with a yield of 10 mg per liter was isolated from the culture medium of the mouldTrichoderma viride. The protein, which we named viridin, was purified by carboxymethyl-cellulose cation-exchange chromatography and Superose 12 HR 10/30 high-performance liquid chromatography. Viridin, a basic protein of approximately 65 kDa as determined by SDS-PAGE, inhibits the growth of the cotton pathogenVerticillum dahliae, the IC50being 6 ΜM.

Isolation and characterization of highly thermophilic xylanolyticThermus thermophilusstrains from hot composts

2000 ◽  
Vol 46 (11) ◽  
pp. 1029-1035 ◽  
Author(s):  
Pierre-François Lyon ◽  
Trello Beffa ◽  
Michel Blanc ◽  
Georg Auling ◽  
Michel Aragno
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Xylanase Activity ◽  
Thermostable Enzyme ◽  
Aerobic Bacteria ◽  
Maximal Growth ◽  
Detailed Report ◽  
Xylanolytic Activity ◽  
Isolation And Characterization

This is the first detailed report of xylanolytic activity in Thermus strains. Two highly thermophilic xylanolytic bacteria, very closely related to non-xylanolytic T. thermophilus strains, have been isolated from the hottest zones of compost piles. Strain X6 was investigated in more detail. The growth rate (optical density monitoring) on xylan was 0.404·h-1at 75°C. Maximal growth temperature was 81°C. Xylanase activity was mainly cell-bound, but was solubilized into the medium by sonication. It was induced by xylan or xylose in the culture medium. The temperature and pH optima of the xylanases were determined to be around 100°C and pH 6, respectively. Xylanase activity was fairly thermostable; only 39% of activity was lost after an incubation period of 48 h at 90°C in the absence of substrate. Xylanolytic T. thermophilus strains could contribute to the degradation of hemicellulose during the thermogenic phase of industrial composting.Key words: Thermus, thermophilic aerobic bacteria, xylanase, thermostable enzyme, compost.

scholarly journals Isolation and characterization of extracellular vesicles from Broncho-alveolar lavage fluid: a review and comparison of different methods

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Jonathan M. Carnino ◽  
Heedoo Lee ◽  
Yang Jin
Keyword(s):  
Extracellular Vesicles ◽  
Culture Medium ◽  
Bronchoalveolar Lavage Fluid ◽  
Cell Communication ◽  
Lavage Fluid ◽  
Surface Marker ◽  
Cell Culture Medium ◽  
Isolation And Characterization ◽  
Insight Into

Abstract Extracellular vesicles (EVs) are cell-derived membranous vesicles secreted by cells into the extracellular space, which play a role in cell to cell communication. EVs are categorized into 3 groups depending on their size, surface marker, and method of release from the host cell. Recently, EVs have become of interest in the study of multiple disease etiologies and are believed to be potential biomarkers for many diseases. Multiple different methods have been developed to isolate EVs from different samples such as cell culture medium, serum, blood, and urine. Once isolated, EVs can be characterized by technology such as nanotracking analysis, dynamic light scattering, and nanoscale flow cytometry. In this review, we summarize the current methods of EV isolation, provide details into the three methods of EV characterization, and provide insight into which isolation approaches are most suitable for EV isolation from bronchoalveolar lavage fluid (BALF).

scholarly journals Studies on Tetanus Toxin and Toxid. II. Isolation and Characterization of the Tetanus Toxin and Toxoid

1968 ◽  
Vol 21 (3) ◽  
pp. 559 ◽  
Author(s):  
DJ Dawson ◽  
CM Mauritzen
Keyword(s):  
Amino Acids ◽  
Tetanus Toxoid ◽  
Tetanus Toxin ◽  
Culture Medium ◽  
Deae Cellulose ◽  
Ph Values ◽  
Neutral Ph ◽  
Isolation And Characterization

Crude tetanus toxin and toxoid were prepared by methanol precipitation. The toxin was purified by a combination of TEAE�cellulose and Sephadex G�200 chromatography at pH values less than 6� o. The toxoid was purified by DEAE� cellulose at approximately neutral pH values. The nature and amount of amino acids of the culture medium which had condensed with the tetanus toxoid proteins during detoxification with formaldehyde was determined.

Isolation and Characterization of Magbane, a Magnesium-Lethal Mutant of Paramecium

Genetics ◽  
2001 ◽  
Vol 158 (3) ◽  
pp. 1061-1069
Author(s):  
Jocelyn A Hammond ◽  
Robin R Preston
Keyword(s):  
Culture Medium ◽  
Single Gene ◽  
Wild Type ◽  
Lethal Mutant ◽  
Isolation And Characterization ◽  
Highly Sensitive ◽  
K Current ◽  
Mutant Phenotypes ◽  
Genetic Mutants

Abstract Discerning the mechanisms responsible for membrane excitation and ionic control in Paramecium has been facilitated by the availability of genetic mutants that are defective in these pathways. Such mutants typically are selected on the basis of behavioral anomalies or resistance to ions. There have been few attempts to isolate ion-sensitive strains, despite the insights that might be gained from studies of their phenotypes. Here, we report isolation of “magbane,” an ion-sensitive strain that is susceptible to Mg2+. Whereas the wild type tolerated the addition of ≥20 mm MgCl2 to the culture medium before growth was slowed and ultimately suppressed (at >40 mm), mgx mutation slowed growth at 10 mm. Genetic analysis indicated that the phenotype resulted from a recessive single-gene mutation that had not been described previously. We additionally noted that a mutant that was well described previously (restless) is also highly sensitive to Mg2+. This mutant is characterized by an inability to control membrane potential when extracellular K+ concentrations are lowered, due to inappropriate regulation of a Ca2+-dependent K+ current. However, comparing the mgx and rst mutant phenotypes suggested that two independent mechanisms might be responsible for their Mg2+ lethality. The possibility that mgx mutation may adversely affect a transporter that is required for maintaining low intracellular Mg2+ is considered.

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