scholarly journals An Improved Sandwich ELISA Method for the Determination of Immunoreactive Schizophyllan (SPG).

1994 ◽  
Vol 17 (11) ◽  
pp. 1437-1440 ◽  
Author(s):  
Akio HIRATA ◽  
Makiko KOMODA ◽  
Wataru ITOH ◽  
Kengo TABATA ◽  
Shinji ITOYAMA ◽  
...  
Keyword(s):  
Sandwich Elisa ◽  
Elisa Method

scholarly journals Validation of method for the determination of bovine casein in milk and milk products

2018 ◽  
Vol 1 (1) ◽  
pp. 18-24
Author(s):  
Minh Hoa Nguyen Thi ◽  
Ha Binh Nguyen Thị ◽  
Son Tran Cao ◽  
◽  
◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Sandwich Elisa ◽  
Food Supplement ◽  
Elisa Method ◽  
Chromatography Method ◽  
Validation Data ◽  
Liquid Chromatography Method ◽  
Labeling Requirements

Bovine caseins in dairy and dairy products were determined by high performance liquid chromatography (HPLC) with a PDA detector at 280 nm or by sandwich ELISA method. Liquid chromatography method was developed to determine different types of casein including alpha, beta, and kappa casein at high content (% level), which calculate the proportion of casein forms, thus allow the identification of milk sources (cow's milk, goat's milk). The ELISA method determines casein at low content (LOD was of 3mg/kg) which meets the labeling requirements of EU and Japan for food allergens. The validation data show that both methods fit the purpose with the recovery of 78-98% and the coefficient of variation is from 2.4 to 9.5%. The methods have been applied for the determination of casein content in 20 samples of milk, food supplement, cake and candy from Hanois' markets.

Determination of Cardiac Disease Biomarker by Plasmonic Sandwich‐ELISA

2020 ◽  
Author(s):  
Weirong Wei ◽  
Yinyan Tang ◽  
Huimin He ◽  
Subash C.B. Gopinath ◽  
Lingling Wang
Keyword(s):  
Cardiac Disease ◽  
Sandwich Elisa ◽  
Disease Biomarker

Rapid and sensitive ELISA method for the determination of bovine somatotropin in blood and milk

1990 ◽  
Vol 38 (6) ◽  
pp. 1358-1362 ◽  
Author(s):  
Craig M. Zwickl ◽  
Holly W. Smith ◽  
Peter H. Bick
Keyword(s):  
Bovine Somatotropin ◽  
Elisa Method

An immunological determination of somatostatin in pharmaceutical by sandwich ELISA based on IgY and polyclonal antibody

2019 ◽  
Vol 145 ◽  
pp. 532-538
Author(s):  
Yingshan Chen ◽  
Yuxin Liang ◽  
Rui Lv ◽  
Nana Xia ◽  
Tianjian Xue ◽  
...  
Keyword(s):  
Polyclonal Antibody ◽  
Sandwich Elisa

Determination of Aflatoxin M1 in Raw Milk by the ELISA Method in the Una-Sana Canton

2020 ◽  
pp. 260-267
Author(s):  
Huska Jukić ◽  
Samira Dedić ◽  
Miloš Rodić ◽  
Zlatko Jusufhodžić ◽  
Dinko Demirović
Keyword(s):  
Raw Milk ◽  
Aflatoxin M1 ◽  
Elisa Method

scholarly journals Fascin-1 and its role as a serological marker in prostate cancer: a prospective case–control study

2021 ◽  
pp. FSO745
Author(s):  
Octavian Sabin Tătaru ◽  
Orsolya Martha ◽  
Felice Crocetto ◽  
Biagio Barone ◽  
Septimiu Voidazan ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Case Control Study ◽  
Sandwich Elisa ◽  
Case Control ◽  
Elisa Method ◽  
Diagnostic Role ◽  
The Difference ◽  
Blood Specimens ◽  
Control Study

Aim: This study aims to investigate any modification of serological FSCN1 in prostate cancer patients compared with patients without neoplasia. Material & methods: Clinical data and blood specimens from patients with and without prostate cancer were obtained. A quantitative sandwich ELISA method was used to determine serological values of FSCN1. Results: Although serum values of FSCN1 were dissimilar in the two cohorts of patients (6.90 vs 7.33 ng/ml), the difference was not statistically significant (p = 0.20). Serum values of FSCN1 stratified for Gleason score groups were not significantly distinguishable (p = 0.65). A negative correlation (rho = -0.331; p = 0.009) was reported between FSCN1 and age. Conclusion: Further studies are required to evaluate a possible diagnostic role of FSCN1 in prostate cancer.

Rapid Detection of Salmonella spp. in Foods by Combination of a New Selective Enrichment and a Sandwich ELISA Using Two Monoclonal Antibodies against Dulcitol 1-Phosphate Dehydrogenase

1996 ◽  
Vol 59 (11) ◽  
pp. 1158-1163 ◽  
Author(s):  
HUAIZE TIAN ◽  
TAKAHISA MIYAMOTO ◽  
TAKASHI OKABE ◽  
YOICHIRO KURAMITSU ◽  
KEN-ICHI HONJOH ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rapid Detection ◽  
Enrichment Culture ◽  
Sandwich Elisa ◽  
False Negative ◽  
Detection Sensitivity ◽  
Elisa Method ◽  
Salmonella Spp ◽  
Selective Enrichment ◽  
Raw Foods

A rapid-detection method was developed for food-borne dulcitol-positive Salmonella spp. in foods that involves a new preenrichment and selective enrichment system and a sandwich ELISA using two monoclonal antibodies against dulcitol 1-phosphate dehydrogenase. Preenrichment and selective enrichment were in Enterobacteriaceae enrichment mannitol (EEM) broth at 42°C for 6 h and in a new dulcitol-magnesium chloride-pyridinesulfonic acid brilliant green-novobiocin (DMPBN) medium at 42°C for 27 h, respectively. The cells were collected from the selective enrichment culture and suspended in 0.1 ml of 1 N NaOH for 2 min. The solution was neutralized with 0.1 ml of 2 M Tris-HCl buffer (pH 7.5) and the mixture was used as a sample for ELISA. The detection sensitivity of the ELISA was 105 CFU of Salmonella spp. per ml of culture. Competing non-Salmonella organisms in raw food did not interfere with the detection of Salmonella cells even when present at 107: 1 (non-Salmonella: Salmonella ratio) in food. Nonmotile Salmonella gallinarum was detected by the ELISA. The minimum detectable number of initial inoculum of Salmonella typhimurium was 0.69 CFU/25 g of raw chicken after the preenrichment in EEM broth and the selective enrichment in DMPBN medium. The present ELISA method required a total analysis time of 36 h including the preenrichment and selective enrichment periods. The ELISA method was compared with a conventional cultural method for the detection of Salmonella cells in 130 samples of raw foods. Of the samples tested, 16 were Salmonella-positive and 114 samples were negative by both methods. False-positive and false-negative results were not encountered.

scholarly journals A Sandwich ELISA for the Determination of Beef Meat Content in Processed Foods

2009 ◽  
Vol 15 (6) ◽  
pp. 613-618 ◽  
Author(s):  
Satoshi KOTOURA ◽  
Yukie MURAKAMI-YAMAGUCHI ◽  
Miyuki NAKAMURA ◽  
Kiyotaka MIAKE ◽  
Masaaki SUGIYAMA ◽  
...  
Keyword(s):  
Sandwich Elisa ◽  
Processed Foods ◽  
Beef Meat ◽  
Meat Content
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