Rapid and sensitive ELISA method for the determination of bovine somatotropin in blood and milk

1990 ◽  
Vol 38 (6) ◽  
pp. 1358-1362 ◽  
Author(s):  
Craig M. Zwickl ◽  
Holly W. Smith ◽  
Peter H. Bick
Keyword(s):  
Bovine Somatotropin ◽  
Elisa Method

Determination of Aflatoxin M1 in Raw Milk by the ELISA Method in the Una-Sana Canton

2020 ◽  
pp. 260-267
Author(s):  
Huska Jukić ◽  
Samira Dedić ◽  
Miloš Rodić ◽  
Zlatko Jusufhodžić ◽  
Dinko Demirović
Keyword(s):  
Raw Milk ◽  
Aflatoxin M1 ◽  
Elisa Method

scholarly journals Development of a C3c-based ELISA method for the determination of anti-complementary potency of Bupleurum polysaccharides

2015 ◽  
Vol 5 (4) ◽  
pp. 316-322 ◽  
Author(s):  
Mulu Wu ◽  
Hong Li ◽  
Yunyi Zhang ◽  
Daofeng Chen
Keyword(s):  
Elisa Method

Determination of aflatoxin B1levels in Iranian and Indian spices by ELISA method

Toxin Reviews ◽  
2014 ◽  
Vol 33 (4) ◽  
pp. 151-154 ◽  
Author(s):  
Amir Sasan Mozaffari Nejad ◽  
Masoud Sabouri Ghannad ◽  
Abolfazl Kamkar
Keyword(s):  
Elisa Method

scholarly journals METHODS FOR ASSESSMENT OF THE SPECIFIC ACTIVITY OF DRUGS FOR PREVENTION OF HEPATITIS B

2017 ◽  
Vol 62 (5) ◽  
pp. 233-240
Author(s):  
E. L. Postnova ◽  
N. V. Shalunova ◽  
K. A. Sarkisyan ◽  
A. A. Movsesyants
Keyword(s):  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Specific Activity ◽  
Elisa Method ◽  
Normative Documents ◽  
Immunologic Activity

The immunologic activity (specific activity) is one of the main indicators of quality of vaccines for prophylaxis of hepatitis B, along with their safety. Retrospective analysis of the use of laboratory methods for assessment of specific (immunogenic) activity of modern vaccines against hepatitis B using indicators was carried out: in vitro method based on evaluation of HBsAg content and in vivo method based on evaluation of immunogenic activity in mice. Both methods are standardized and described in normative documents on the vaccines against hepatitis B of domestic production registered in the Russian Federation. Indicators of specific (immunogenic) activity of vaccines against hepatitis B were used to investigate more than 170 vaccine series using the ELISA method in the period from 2013 to 2015. The obtained control results confirmed the expediency and efficiency of enzyme immunoassay for determination of HBsAg content, as well as permissibility of use of ready sets of the Murex HBsAg Version 3 test systems for testing vaccines against hepatitis B by the ELISA method. Analysis of the results of laboratory control of series of vaccines against hepatitis B using a biological method for immunogenicity evaluation based on ED50 analysis confirms persistently high immunogenic activity of the Russian commercial vaccines intended for prophylaxis of hepatitis B. The confirmed comparability of methods allows the number of in vivo tests to be further reduced in favor of the enzyme immunoassay authentically characterizing the produced drug.

scholarly journals Development of MNPs Based Enzyme Immuno-Sorbent Analysis for the Determination of Organophosphorus Pesticides in Milk

2019 ◽  
Vol 13 (1) ◽  
pp. 146-154
Author(s):  
Yavor Ivanov
Keyword(s):  
Magnetic Nanoparticles ◽  
Organophosphorus Pesticides ◽  
Simple Procedure ◽  
Antibody Immobilization ◽  
Elisa Method ◽  
Standard Curve ◽  
Milk Samples ◽  
Conventional Elisa ◽  
Reliable Methods

Objectives: High toxicity of pesticides requires accurate and reliable methods to monitor their levels for food, water and soil safety. Methods: Magnetic nanoparticles were synthesized and functionalized with (3-aminopropyl)triethoxysilane. Sheep polyclonal-anti-paraoxon and anti-dichlorvos antibodies were produced. Dichlorvos-cationized ovalbumin-HPR and paraoxon-ovalbumin-HPR conjugates were prepared and characterized. The optimal conditions for antibody immobilization were determined. Enzyme immunosorbent assay based on Magnetic Nanoparticles (MNPs) for the determination of pesticides was developed. A comparison of the developed modified MNPs-based ELISA with the conventional ELISA was performed. The effect of protein, pH, and fats in milk samples on the analytical characteristics of the MNPs-based ELISA was investigated. In order to validate the results obtained from the MNPs-based ELISA method, experiments with HPLC were performed. Results: The linear range of the standard curve for the determination of paraoxon in buffer with anti-paraoxon antibody was 0.125-5 ng/mL, for the dichlorvos with anti-dichlorvos antibody was 0.0625-5 ng/mL, and for the mixture of paraoxon and dichlorvos in ratio (1:1) with multi-antibody was 0.125-5 ng/mL. A comparison of the developed modified MNPs-based ELISA with the conventional ELISA was performed. The recovery of the obtained results for the determination of pesticides in milk samples in both methods was from 104 to 110%. Conclusion: The stated advantages of magnetic nanoparticles provide good sensitivity and rapidity of immunoassay and a simple procedure for separating of antibody-magnetic nanoparticles with the bound antigen from the non-bound antigen in the sample.

scholarly journals Determination of specific antibodies titre to salmonella enteritidis by elisa technique in several selected flocks of laying hens

2004 ◽  
Vol 58 (3-4) ◽  
pp. 319-326 ◽  
Author(s):  
Maja Velhner ◽  
Dusan Orlic ◽  
Dubravka Potkonjak ◽  
Milos Kapetanov ◽  
Sava Lazic
Keyword(s):  
Antibody Titre ◽  
Salmonella Enteritidis ◽  
Laying Hens ◽  
Elisa Method ◽  
Specific Antibodies ◽  
Elisa Technique ◽  
High Level

In this paper, the antibody titre to Salmonella enteritidis (SE) was examined by the ELISA method in two flocks of laying hens, where during routine bacteriological investigations Salmonellae was never isolated, and in one flock where Colysepticemia was diagnosed and Salmonella isolated accidentally. In the flocks were Salmonellae were not isolated, a titre with a high level of specific antibodies to SE was discovered (15 and 45%), while the flock with accidental findings of SE was poorly positive (5%). These results point to the necessity of introducing serological monitoring to SE so that the infection of salmonella may be discovered early and the prevalence in the flock determined, and also for the purpose of applying adequate measures that could reduce the possibility of secretion of SE through eggs.

scholarly journals First serological record of Coxiella burnetii infection in the equine population of Slovakia

Biologia ◽  
2021 ◽  
Author(s):  
Monika Drážovská ◽  
Marián Prokeš ◽  
Boris Vojtek ◽  
Jana Mojžišová ◽  
Anna Ondrejková ◽  
...  
Keyword(s):  
16S Rrna ◽  
Coxiella Burnetii ◽  
Animal Species ◽  
Q Fever ◽  
Statistical Significance ◽  
Elisa Method ◽  
Zoonotic Pathogen ◽  
Specific Antibodies

AbstractCoxiella burnetii is a worldwide zoonotic pathogen causing Q fever in various animal species and humans. In Slovakia, cases of C. burnetii infection in both animals and humans are confirmed every year. The role of horses in the epidemiology of this neglected disease is still unclear. In our study, we focused on a serosurvey of C. burnetii in the equine population in Slovakia by the ELISA method. Subsequently, a nested PCR was performed to detect the 16S rRNA fragment of the genus Coxiella. Among 184 horse sera, the presence of specific antibodies to C. burnetii was detected in four samples, representing a 2.17% seropositivity. All the positive horses were mares; two originated from Central Slovakia and two from Eastern Slovakia. Although the number of positive samples was too small for a determination of statistical significance, our results provide the first confirmation of antibodies to C. burnetii in horses from Slovakia. Although no positive PCR result was obtained, these serological findings may help to clarify the circulation of the pathogen in the environment.

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