The use of microtiter plates for the simple and sensitive determination of insulin by an ELISA method

1988 ◽  
Vol 44 (10) ◽  
pp. 877-879 ◽  
Author(s):  
I. Angel
Keyword(s):  
Elisa Method ◽  
Microtiter Plates ◽  
Sensitive Determination

A New, Rapid And Sensitive Determination Of Fibrinopeptide- A (FPA) By A High Affinity Antibody To FPA Antiserum

1981 ◽  
Author(s):  
G Stehle ◽  
J Harenberg ◽  
H Schmidtgayk ◽  
R Zimmermann
Keyword(s):  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Standard Curve ◽  
High Affinity ◽  
Microtiter Plates ◽  
Highly Sensitive ◽  
Ethanolic Extraction ◽  
Sensitive Determination ◽  
Radioimmunological Determination

The clinical relevance of the determination of FPA is not yet fully recognized because of the still time consuming radioimmunological techniques. Shortening of the incubation times allways lead to a critical loss of the sensitivity of the assay. We present now a modification which provides highly sensitive and reproducable results within 2hrs.The ethanolic extraction of FPA from plasma was shortened to 20 min including centrifugation. Samples were analysed in triplicates and evaporated at 50°C on microtiter plates. The addition of 0.2μl normal rabbit serum (NRS) lead to a 20% increase of the reaction rate of the FPA antiserum to FPA. A second antibody with high affinity to rabbit immunglobulin i.e. the FPA antiserum improved the maximal binding to 35% after an incubation period of only 10 min. 35-40000 cpm tracer FPA were added to each sample. FPA antiserum, second antibody and NRS were preincubated for 1-24 hrs and then added together with the tracer to the samples. Thus a sensitive standard curve was obtained between 0.16 and 160 ng/ml (12000-600 cpm). The correlation coefficient of this modification to our previously described method was r=0.96 (n=60) The variation coefficient could be improved substantially to 3.1 for low, 4.0 for medium and 4.5% for high FPA. Normal FPA were measured between 0.16 and 2.5 ng/ml (mean 1.4 ng/ml, n=32).The presented modification of the radioimmunological determination of FPA overcomes the difficulties of previously described methods and provides acurate results within 2 hrs.

Development of a colorimetric protein phosphorylation assay for detecting cyanobacterial toxins

1995 ◽  
Vol 31 (5-6) ◽  
pp. 47-49 ◽  
Author(s):  
C. Ash ◽  
C. MacKintosh ◽  
R. MacKintosh ◽  
C. R. Fricker
Keyword(s):  
Protein Phosphorylation ◽  
Colorimetric Assay ◽  
Cyanobacterial Toxins ◽  
Sensitive Determination

A new colorimetric assay is described, based on inhibition of protein phosphotases, that enables the rapid, simple and sensitive determination of the concentration of toxins from cyanobacteria.

Non-enzymatic determination of purine nucleotides using a carbon dot modified glassy carbon electrode

2019 ◽  
Vol 11 (30) ◽  
pp. 3866-3873 ◽  
Author(s):  
R. Karthikeyan ◽  
D. James Nelson ◽  
S. Abraham John
Keyword(s):  
Glassy Carbon ◽  
Low Cost ◽  
Phosphate Buffer Solution ◽  
Purine Nucleotides ◽  
Buffer Solution ◽  
Solution Ph ◽  
Carbon Dot ◽  
Enzymatic Determination ◽  
Sensitive Determination

Selective and sensitive determination of one of the purine nucleotides, inosine (INO) using a low cost carbon dot (CD) modified glassy carbon (GC) electrode in 0.2 M phosphate buffer solution (pH 7.2) was demonstrated in this paper.

Cross Double Point Discharge as Enhanced Excitation Source for Highly Sensitive Determination of Arsenic, Mercury and Lead by Optical Emission Spectrometry

2021 ◽  
Author(s):  
Lin He ◽  
Peixia Li ◽  
Kai Li ◽  
Tao Lin ◽  
Jin Luo ◽  
...  
Keyword(s):  
Double Point ◽  
Hydride Generation ◽  
Optical Emission ◽  
Sample Introduction ◽  
Excitation Source ◽  
Emission Spectrometry ◽  
Highly Sensitive ◽  
Point Discharge ◽  
Sensitive Determination

A new cross double point discharge (CrossPD) microplasma was designed as an excitation source to construct a miniaturized optical emission spectrometer with hydride generation (HG) for sample introduction. The CrossPD...

scholarly journals Fast and Sensitive Determination of the Fungicide Carbendazim in Fruit Juices with an Immunosensor Based on White Light Reflectance Spectroscopy

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 153
Author(s):  
Georgios Koukouvinos ◽  
Chrysoula-Evangelia Karachaliou ◽  
Ioannis Raptis ◽  
Panagiota Petrou ◽  
Evangelia Livaniou ◽  
...  
Keyword(s):  
White Light ◽  
Optical Biosensor ◽  
Reflectance Spectroscopy ◽  
Fruit Juices ◽  
Sample Treatment ◽  
Assay Sensitivity ◽  
Reliable Determination ◽  
Light Reflectance ◽  
Sensitive Determination

Carbendazim is a systemic benzimidazole-type fungicide with broad-spectrum activity against fungi that undermine food products safety and quality. Despite its effectiveness, carbendazim constitutes a major environmental pollutant, being hazardous to both humans and animals. Therefore, fast and reliable determination of carbendazim levels in water, soil, and food samples is of high importance for both food industry and public health. Herein, an optical biosensor based on white light reflectance spectroscopy (WLRS) for fast and sensitive determination of carbendazim in fruit juices is presented. The transducer is a Si/SiO2 chip functionalized with a benzimidazole conjugate, and determination is based on a competitive immunoassay format. Thus, for the assay, a mixture of an in-house developed rabbit polyclonal anti-carbendazim antibody with the standards or samples is pumped over the chip, followed by biotinylated secondary antibody and streptavidin. The WLRS platform allows for real-time monitoring of biomolecular interactions carried out onto the Si/SiO2 chip by transforming the shift in the reflected interference spectrum caused by the immunoreaction to effective biomolecular adlayer thickness. The sensor is able to detect 20 ng/mL of carbendazim in fruit juices with high accuracy and precision (intra- and inter-assay CVs ≤ 6.9% and ≤9.4%, respectively) in less than 30 min, applying a simple sample treatment that alleviates any “matrix-effect” on the assay results and a 60 min preincubation step for improving assay sensitivity. Excellent analytical characteristics and short analysis time along with its small size render the proposed WLRS immunosensor ideal for future on-the-spot determination of carbendazim in food and environmental samples.

scholarly journals Sensitive determination of gentamicin in plasma using ion-exchange solid-phase extraction followed by UHPLC-MS/MS analysis

2021 ◽  
pp. e00246
Author(s):  
Ana Laura Anibaletto dos Santos ◽  
Anne Caroline Cezimbra da Silva ◽  
Lilian de Lima Feltraco Lizot ◽  
Anelise Schneider ◽  
Roberta Zilles Hahn ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Ms Analysis ◽  
Sensitive Determination
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