Tissue interactions in the organization and maintenance of the muscle pattern in the chick limb

Development ◽  
1983 ◽  
Vol 76 (1) ◽  
pp. 199-215
Author(s):  
Annick Mauger ◽  
Madeleine Kieny ◽  
Ihsan Hedayat ◽  
Paul F. Goetinck
Keyword(s):  
Chick Embryos ◽  
Limb Muscle ◽  
Collagen Biosynthesis ◽  
In Ovo ◽  
Avian Embryos ◽  
Tissue Interactions ◽  
Pattern Development ◽  
Somitic Mesoderm ◽  
Putative Mutant ◽  
Muscular Dysgenesis

Recent investigations on a hereditary muscular dysgenesis (cn/cn) in the chicken (Kieny, Mauger, Hedayat & Goetinck, 1983) have suggested that limb muscle pattern development and subsequent maintenance are two independent steps in the formation of the musculature. The respective activities or muscle cells and connective tissue cells in the ontogeny of the musculature have been investigated in avian embryos 1) by in ovo administration of drugs interfering with collagen biosynthesis, and 2) by heterogenetic somite-exchange experiments between normal and mutant embryos. None of the drugs administered to the chick embryo caused any disturbance of muscle pattern formation or maintenance whether treatment occurred before (5 days) or after (7·5 days) the muscle splitting period. Heterogenetic implantations were performed at 2 days of incubation either at the leg or at the wing level. Somitic mesoderm from non-mutant quail embryo was grafted to replace a piece of somitic mesoderm in putative mutant (cn/cn) chick embryos. The introduction of normal myogenic cells into a mutant leg or wing led to a normally patterned musculature, which demonstrates that the muscular dysgenesis cn/cn results from a defect of the somitic myogenic cell line.

Mouse chick chimera: a new model to study the in ovo developmental potentialities of mammalian somites

Development ◽  
1995 ◽  
Vol 121 (6) ◽  
pp. 1705-1718 ◽  
Author(s):  
J. Fontaine-Perus ◽  
V. Jarno ◽  
C. Fournier le Ray ◽  
Z. Li ◽  
D. Paulin
Keyword(s):  
In Vitro Cultures ◽  
Mouse Embryos ◽  
Limb Muscle ◽  
Mammalian Development ◽  
In Ovo ◽  
Gene Coding ◽  
Myogenic Stem Cells ◽  
Mouse Cells ◽  
Beta Galactosidase

Chimeras were prepared by transplanting somites from 9-day post-coitum mouse embryos or somitic dermomyotomes from 10-day post-coitum mouse embryos into 2-day-old chick embryos at different axial levels. Mouse somitic cells then differentiated in ovo in dermis, cartilage and skeletal muscle as they normally do in the course of development and were able to migrate into chick host limb. To trace the behavior of somitic myogenic stem cells more closely, somites arising from mice bearing a transgene of the desmin gene linked to a reporter gene coding for Escherichia coli beta-galactosidase (lacZ) were grafted in ovo. Interestingly, the transgene was rapidly expressed in myotomal muscles derived from implants. In the limb muscle mass, positive cells were found several days after implantation. Activation of desmin nls lacZ also occurred in in vitro cultures of somite-derived cells. Our experimental method facilitates investigation of the mechanisms of mammalian development, allowing the normal fate of implanted mouse cells to be studied and providing suitable conditions for identification of descendants of genetically modified cells.

Differentiation of allantoic endoderm implanted into the presumptive digestive area in avian embryos. A study with organ-specific antigens

Development ◽  
1984 ◽  
Vol 80 (1) ◽  
pp. 137-153
Author(s):  
Sadao Yasugi
Keyword(s):  
Digestive Tract ◽  
Intestinal Epithelium ◽  
Digestive Organ ◽  
Chick Embryos ◽  
Avian Embryos ◽  
Digestive Organs ◽  
Organ Specific ◽  
Specific Antigens

Quail allantoic endoderm was implanted into the presumptive digestive-tract area of chick embryos, and the differentiation of the endoderm was examined morphologically and immunocytochemically with antisera against pepsinogens and sucrase. The allantoic endoderm was incorporated into the host digestive organs. It often became continuous with the host endoderm and formed a chimaeric digestive-tract epithelium. It differentiated morphologically into the epithelium of the digestive organ into which it was incorporated, showing the morphological inductive ability in situ of the digestive-tract mesenchyme against the allantoic endoderm. However, the allantoic endoderm did not produce pepsinogens even when it was incorporated into the host proventricular mesenchyme and formed well-developed proventricular glands. This result indicates that the heterotypic morphogenesis of the allantoic endoderm is not necessarily accompanied by the heterotypic cytodifferentiation. In contrast, the anti-sucrase antiserum-reactive cells often differentiated in the allantoic endoderm incorporated into not only the intestine but also other organs. This confirmed our previous observation that the allantoic endoderm has a tendency to differentiate into the intestinal epithelium in the heterologous environment.

The acoustocardiogram: a noninvasive method for measuring heart rate of avian embryos in ovo

1990 ◽  
Vol 69 (4) ◽  
pp. 1546-1548 ◽  
Author(s):  
H. Rahn ◽  
S. A. Poturalski ◽  
C. V. Paganelli
Keyword(s):  
Heart Rate ◽  
Low Cost ◽  
Noninvasive Method ◽  
In Ovo ◽  
Avian Embryos ◽  
Condenser Microphone ◽  
Avian Eggs

A method is presented for measuring the heart rate of avian eggs noninvasively during the last half of incubation. The technique involves briefly placing an egg in tightly sealed vessel containing an inexpensive condenser microphone. The amplified output of the microphone, termed the acoustocardiogram (ACG), is nearly sinusoidal in shape and synchronous with the electrocardiogram. The ACG can also be obtained by mounting the microphone directly on the shell with Plasticine. The method offers advantages over previously described techniques in simplicity, low cost, and noninvasiveness.

scholarly journals Effect of in ovo feeding of L-glutamine + ISP to chick embryos

2019 ◽  
Vol 13 (4) ◽  
pp. 591
Author(s):  
João Paulo Ferreira Rufino ◽  
Frank George Guimarães Cruz ◽  
Valcely Da Rocha Costa ◽  
André Ferreira Silva ◽  
Pedro Alves de Oliveira Filho ◽  
...  
Keyword(s):  
Chick Embryos ◽  
In Ovo ◽  
In Ovo Feeding

The spatial and temporal distribution of polarizing activity in the flank of the pre-limb-bud stages in the chick embryo

Development ◽  
1991 ◽  
Vol 111 (3) ◽  
pp. 725-731 ◽  
Author(s):  
A. Hornbruch ◽  
L. Wolpert
Keyword(s):  
Posterior Part ◽  
Temporal Distribution ◽  
Early Embryo ◽  
Limb Bud ◽  
Posterior Edge ◽  
Limb Buds ◽  
Avian Embryos ◽  
Low Levels ◽  
Clear Change ◽  
Somitic Mesoderm

The presence of polarizing activity in the limb buds of developing avian embryos determines the pattern of the anteroposterior axis of the limbs in the adult. Maps of the spatial distribution and the strength of the signal within limb buds of different stages are well documented. Polarizing activity can also be found in Hensen's node in the early embryo. We have mapped the distribution of polarizing activity as it emerges from Hensen's node and spreads into the flank tissue of the embryo. There is a clear change in the local pattern of expression of polarizing activity between stage 8 and 18. Almost no activity is measured for stages 8 and 9. More or less uniform levels of around 10% are spread along the flank lateral to the unsegmented somitic mesoderm from somite position 12 to 22 in stage 10 embryos. Some 6 to 8 h later at stage 12, there is a distinct peak of activity at somite position 18, the middle of the wing field. This peak increases at stages 13 to 15 and its position traverses to the posterior edge of the wing field. Full strength of activity is reached shortly before the onset of limb bud formation at stage 16 to 17. Stages 16 to 18 were investigated for polarizing activity in the wing and the leg field. Low levels of polarizing activity are present in the anterior leg field at stages 16 and 17 but have disappeared by stage 18 and all activity is confined to the posterior part of the leg bud.

Rôle du mésoderme somitique dans le développement du plumage dorsal chez l'embryon de Poulet

Development ◽  
1972 ◽  
Vol 28 (2) ◽  
pp. 343-366
Author(s):  
Par Annick Mauger
Keyword(s):  
Early Stage ◽  
Cervical Region ◽  
Lumbar Region ◽  
Chick Embryos ◽  
A Chain ◽  
Normal Differentiation ◽  
Time Of Operation ◽  
Somitic Mesoderm ◽  
Axial Organs

The role of somitic mesoderm in the development of dorsal plumage in chick embryos. II. Regionalisation. Transplantation and inversion experiments were performed on the somitic mesoderm of 2- to 2·5-day chick embryos in order to study the role of regional and axial determinations in the development of the dorsal plumage. The transposition of a piece of somitic mesoderm from the posterior cervical region (where the spinal pteryla is narrow) to the thoraco-lumbar region (where it is wide) leads to a local and unilateral narrowing of the spinal pteryla at the operation site. Conversely, the transposition of somitic mesoderm from the thoraco-lumbar region to the posterior cervical region results in a local and unilateral widening of the spinal pteryla. Consequently at the time of operation the segmented or not yet segmented somitic mesoderm is already determined to give rise to a definite transverse level of the spinal pteryla. The inversion of the cephalo-caudal polarity of a piece of somitic mesoderm without the ectodermal covering, or of a portion of the axial organs deprived of the overlying ectoderm has no effect on the orientation of feather filaments and feather rows. In contrast, the inversion of the cephalo-caudal polarity of a portion of the axial organs together with the overlying ectoderm results in the development of feathers growing in a cephalad direction and feather chevrons opening towards the head of the embryo. The inversion of the dorso-ventral polarity of a piece of somitic mesoderm does not prevent the normal differentiation of feathers in the operated region. The inversion of the medio-lateral polarity of a piece of unsegmented somitic mesoderm has little effect on the development of the spinal pteryla. On the contrary, the medio-lateral inversion of a chain of somites precludes the formation of the feathers at the level of operation. The somitic mesoderm, even when segmented, is endowed with extensive regulative capacity of its axes, except for the medio-lateral polarity, which is fixed irreversibly at the time of segmentation. The regional determination of the feather-forming somitic mesoderm is acquired at an early stage, at any rate before segmentation. However, at a given transverse level of the cephalo-caudal axis, the somitic cells remain totipotent as concerns their histo-genetic destiny (dermatome, myotome, or sclerotome) until after the onset of segmentation.

Experimental analysis of control mechanisms in somite segmentation in avian embryos.

Development ◽  
1983 ◽  
Vol 74 (1) ◽  
pp. 1-14
Author(s):  
Marianne Veini ◽  
Ruth Bellairs
Keyword(s):  
Coturnix Japonica ◽  
Control Mechanisms ◽  
Coturnix Coturnix Japonica ◽  
In Ovo ◽  
Avian Embryos ◽  
Coturnix Coturnix ◽  
Reduced Body ◽  
Somite Formation ◽  
The Individual ◽  
Area Pellucida

The blastulae of unincubated eggs of the quail, Coturnix coturnix japonica, have been bisected in ovo, using the technique of Lutz (1949). Some embryos were harvested after 24 hand found to possess two primitive streaks. Most were fixed at 48 h or 72 h. Some were found to have regulated to form almost normal single axes, whilst others had developed into duplicitas anterior embryos, separate twins or collided axes. All three types of twinned embryos were smaller than the control embryos. The number of somites was not however reduced in the shorter embryos. This finding corresponds with a similar result obtained by Cooke (1975) who reported that if a Xenopus blastula is reduced in size, it nevertheless develops the correct number of somites. The quail however adjusts the shape of the individual somites so that they fit into the reduced body length, whereas Xenopus reduces the size of somites. No miniaturized somites were ever seen in these quail embryos. As a result of the present experiments, it was concluded that the length of incubation time does not directly control the rate of somite formation, because different numbers of somite were found in twins which possessed identical genomes and had developed in almost the same environment for identical periods. In addition, the size of the area pellucida does not appear to control somite formation. Probably, the most important influence is the regression of the node.

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