Évolution enzymatique des tissus foetaux et placentaires de Rat en carence tératogène de riboflavine

Development ◽  
1974 ◽  
Vol 31 (1) ◽  
pp. 183-198
Author(s):  
G. Potier de Courcy ◽  
S. Desmettre-Miguet ◽  
M. R. Macquart-Moulin ◽  
T. Terroine
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Electrophoretic Pattern ◽  
Heart Tissue ◽  
Yolk Sac ◽  
The Other ◽  
Riboflavin Deficiency ◽  
Other Hand ◽  
Ldh Isozymes

Enzymic changes of foetal and placental tissues of the rat in teratogenic riboflavin deficiency The electrophoretic pattern of alkaline phosphatase was studied in teratogenic riboflavin deficiency in the whole foetus, chorioallantoic placenta and visceral yolk-sac of the rat in order to follow the course of their differentiation from day 13 to day 20 of gestation. Lacticdehydrogenase (LDH) isozymes were chosen in the case of foetal organs (heart, intestine and kidneys) as a more sensitive test. A fluorometric analysis of alkaline phosphatase was also performed to evaluate more precisely quantitative modifications brought on by maternal riboflavin deficiency in the placenta and the whole foetus. The importance of alkaline phosphatase in rat yolk-sac was indicated in the last period of gestation by the presence of a specific rapid isozyme band from the 16th to the 21st day, simultaneously with a greater complexity of the electrophoretic picture of this membrane. Placenta and foetus, on the other hand, were shown to possess two common bands but those concerned with the foetus appeared later and were related to the onset and increase of ossification. In the foetal organs, differentiation of LDH isozymes appeared specific for each organ studied; while the heart tissue showed five bands on the 16th day, kidney arrived at this stage only on the 21st day, indicating a slower maturation of this tissue. At the end of gestation riboflavin deficiency delayed the appearance of rapid isoenzymes of alkaline phosphatase in the whole foetus and of LDH in the foetal kidneys, suggesting a similar delay in the course of metabolic differentiation of the skeleton and kidney tissues. Deficiency had no effect upon placental isoenzyme development but in this organ it greatly reduced the biochemical alkaline phosphatase activity, especially on day 13. This result suggests the participation of the enzyme in the general hypotrophy of the foetus by a decrease in nutrients carried by the placenta. On the other hand, the decline in the alkaline phosphatase activity of the whole foetus is related to the delayed appearance of mineralization.

ÖSTROGENBEDINGTE INDUKTION ALKALISCHER PHOSPHATASE IN FIBROBLASTEN DES SUBCUTANEN BINDEGEWEBES DER MAUS

1968 ◽  
Vol 57 (2) ◽  
pp. 261-273 ◽  
Author(s):  
H. E. Schaefer ◽  
R. Fischer
Keyword(s):  
Alkaline Phosphatase ◽  
Connective Tissue ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
The Other ◽  
Endocrine Function ◽  
Strong Reaction ◽  
Oestrogen Treatment ◽  
Oestradiol Benzoate ◽  
Subcutaneous Connective Tissue

ABSTRACT Alkaline phosphatase activity, demonstrated by cytochemical techniques, is induced by oestrogens in fibroblasts of the subcutaneous connective tissue of mice. As a rule, low or moderate enzymatic activities appear in some subcutaneous fibroblasts of females with the beginning of the endocrine function of the ovary, whereas alkaline phosphatase is completely missing in the subcutaneous fibroblasts of males and castrated females. On the other hand, the application of oestrogenic substances (α- and β-oestradiol, oestradiol benzoate, oestradiol valerinate) produces a strong reaction of alkaline phosphatase in numerous fibroblasts of males and castrated animals. Furthermore, the low enzymatic activity generally existing in the subcutis of intact females is distinctly increased by oestrogen treatment. The alkaline phosphatase activity in fibroblasts, induced by oestrogens, is depending on time and dose of the hormone application.

scholarly journals Lipid deposition in intestine as a possible cause of malabsorption of nutrients in zinc-deficient common carp (Cyprinus carpio)

1994 ◽  
Vol 72 (5) ◽  
pp. 753-762 ◽  
Author(s):  
S.K. Taneja ◽  
P. Arya
Keyword(s):  
Alkaline Phosphatase ◽  
Glucose Uptake ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
The Other ◽  
Zn Deficiency ◽  
Lipid Deposition ◽  
High Lipid ◽  
Triacylglycerol Concentration ◽  
High Lipid Diet

An experiment was performed to examine the interaction between Zn deficiency and lipid intake in carp. The carp were given a high-lipid diet that was either Zn-deficient (ZD) or Zn-supplemented (ZS), or were pair-fed (PF) the ZS diet to the intake of the ZD group. After 8 weeks the carp were killed and measurements were made of intestinal glucose uptake, levels of DNA, RNA and triacylglycerol, and alkaline phosphatase (EC3.1.3.1) activity in liver and intestine samples. A further group of similar carp were given the same diets but at week 8 were transferred to low-lipid diets, with the exception of half the ZD group. After a further 8 weeks of treatment, carps were killed for biochemical studies. Intestinal [14C]glucose uptake, levels of DNA, RNA and alkaline phosphatase activity in intestine and liver were significantly (P< 0·05) lower in the high-lipid ZD group than in the high-lipid ZS and PF diet groups. The triacylglycerol concentration in the intestine was higher in the high-lipid ZD group than in the other two groups. When the carp were given the corresponding low-lipid diets, the variables measured in intestine and liver of the ZD group were close to those of the other groups. The results of this study demonstrate that lipid, when present in excess in the diet, accumulates in the intestine under Zn-deficient conditions and may reduce the absorption of glucose in carp. The reduced RNA and DNA levels and alkaline phosphatase activity in liver and intestine of ZD fish compared with those of ZS fish given high- lipid diets is proposed to be due to the malabsorption of nutrients linked with lipid deposition in the intestine, rather than their dependence on the level of Zn in the diet.

scholarly journals Activity of Enzymes in the Endometrium, Caruncles, and Uterine Rinsings of Progestogen-Treated and Naturally Cycling Ewes

1968 ◽  
Vol 21 (1) ◽  
pp. 123 ◽  
Author(s):  
RN Murdoch ◽  
IG White
Keyword(s):  
Alkaline Phosphatase ◽  
Corpus Luteum ◽  
Succinate Dehydrogenase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Luteal Phase ◽  
The Other ◽  
Glutamate Oxaloacetate Transaminase ◽  
Acid And Alkaline Phosphatase ◽  
Activity Of Enzymes

The activity of several enzymes has been measured in the endometrium, caruncles, and uterine rinsings of ewes at various stages of the oestrous cycle. Ewes were either allowed to cycle naturally or were synchronized by progestogenimpregnated sponges inserted into the vagina. Most (86%) of the progestogentreated ewes came into oestrus 2 or 3 days after removal of the sponges, and the uterus contained higher levels of amylase and alkaline phosphatase than did naturally cycling ewes. Amylase, succinate dehydrogenase, glutamate-oxaloacetate transaminase, acid and alkaline phosphatase activities were maximal during the luteal phase of the cycle. Endometrial alkaline phosphatase activity followed the growth and retrogression of the corpus luteum more closely than that of the other enzymes studied.

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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