scholarly journals Activity of Enzymes in the Endometrium, Caruncles, and Uterine Rinsings of Progestogen-Treated and Naturally Cycling Ewes

1968 ◽  
Vol 21 (1) ◽  
pp. 123 ◽  
Author(s):  
RN Murdoch ◽  
IG White

The activity of several enzymes has been measured in the endometrium, caruncles, and uterine rinsings of ewes at various stages of the oestrous cycle. Ewes were either allowed to cycle naturally or were synchronized by progestogenimpregnated sponges inserted into the vagina. Most (86%) of the progestogentreated ewes came into oestrus 2 or 3 days after removal of the sponges, and the uterus contained higher levels of amylase and alkaline phosphatase than did naturally cycling ewes. Amylase, succinate dehydrogenase, glutamate-oxaloacetate transaminase, acid and alkaline phosphatase activities were maximal during the luteal phase of the cycle. Endometrial alkaline phosphatase activity followed the growth and retrogression of the corpus luteum more closely than that of the other enzymes studied.

Author(s):  
Luciana de Oliveira Franco ◽  
Thayza Christina Montenegro Stamford ◽  
Marcos Antônio Barbosa de Lima ◽  
Rita de Cassia Carvalho Maia ◽  
Tânia Lúcia Montenegro Stamford ◽  
...  

1968 ◽  
Vol 57 (2) ◽  
pp. 261-273 ◽  
Author(s):  
H. E. Schaefer ◽  
R. Fischer

ABSTRACT Alkaline phosphatase activity, demonstrated by cytochemical techniques, is induced by oestrogens in fibroblasts of the subcutaneous connective tissue of mice. As a rule, low or moderate enzymatic activities appear in some subcutaneous fibroblasts of females with the beginning of the endocrine function of the ovary, whereas alkaline phosphatase is completely missing in the subcutaneous fibroblasts of males and castrated females. On the other hand, the application of oestrogenic substances (α- and β-oestradiol, oestradiol benzoate, oestradiol valerinate) produces a strong reaction of alkaline phosphatase in numerous fibroblasts of males and castrated animals. Furthermore, the low enzymatic activity generally existing in the subcutis of intact females is distinctly increased by oestrogen treatment. The alkaline phosphatase activity in fibroblasts, induced by oestrogens, is depending on time and dose of the hormone application.


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