scholarly journals Physical interactions between Gsx2 and Ascl1 balance progenitor expansion versus neurogenesis in the mouse lateral ganglionic eminence

Development ◽  
2020 ◽  
Vol 147 (7) ◽  
pp. dev185348 ◽  
Author(s):  
Kaushik Roychoudhury ◽  
Joseph Salomone ◽  
Shenyue Qin ◽  
Brittany Cain ◽  
Mike Adam ◽  
...  
Keyword(s):  
Ganglionic Eminence ◽  
Lateral Ganglionic Eminence ◽  
Physical Interactions

scholarly journals Physical interactions between Gsx2 and Ascl1 regulate the balance between progenitor expansion and neurogenesis in the mouse lateral ganglionic eminence

2019 ◽  
Author(s):  
Kaushik Roychoudhury ◽  
Joseph Salomone ◽  
Shenyue Qin ◽  
Masato Nakafuku ◽  
Brian Gebelein ◽  
...  
Keyword(s):  
Dna Binding ◽  
Ventricular Zone ◽  
Luciferase Assay ◽  
Proximity Ligation Assay ◽  
Ganglionic Eminence ◽  
Independent Manner ◽  
Helix Loop Helix ◽  
Lateral Ganglionic Eminence ◽  
Physical Interactions

AbstractThe Gsx2 homeodomain transcription factor is required to maintain neural progenitor identity in the lateral ganglionic eminence (LGE) within the developing ventral telencephalon, despite its role in upregulating the neurogenic factor Ascl1. How Gsx2 maintains cells as progenitors in the presence of a pro-differentiation factor is unclear. Here, we show that Gsx2 and Ascl1 are co-expressed in dividing subapical progenitors within the LGE ventricular zone (VZ). Moreover, we show that while Ascl1 misexpression promotes neurogenesis in dorsal telencephalic progenitors that do not express Gsx2, co-expression of Gsx2 with Ascl1 inhibits neurogenesis in these cells. To investigate the mechanisms underlying this inhibition, we used a cell-based luciferase assay to show that Gsx2 reduced the ability of Ascl1 to activate target gene expression in a dose-dependent and DNA binding-independent manner. Yeast 2-hybrid and co-immunoprecipitation assays revealed that Gsx2 physically interacts with the basic-Helix-Loop-Helix (bHLH) domain of Ascl1, and DNA binding assays demonstrated that this interaction interferes with the ability of Ascl1 to form homo- or heterodimers with E-proteins such as Tcf3 on DNA. To further assess for in vivo molecular interactions between these transcription factors within the telencephalon, we modified a proximity ligation assay for embryonic tissue sections and found that Ascl1:Gsx2 interactions are enriched within VZ progenitors, whereas Ascl1:Tcf3 interactions predominate in basal progenitors. Altogether, these findings suggest that physical interactions between Gsx2 and Ascl1 limit Ascl1:Ascl1 and Ascl1:Tcf3 interactions, and thereby inhibit Ascl1-dependennt neurogenesis and allow for progenitor expansion within the LGE.

Regionalization within the mammalian telencephalon is mediated by changes in responsiveness to Sonic Hedgehog

Development ◽  
1998 ◽  
Vol 125 (24) ◽  
pp. 5079-5089 ◽  
Author(s):  
J.D. Kohtz ◽  
D.P. Baker ◽  
G. Corte ◽  
G. Fishell
Keyword(s):  
Basal Ganglia ◽  
Globus Pallidus ◽  
Sonic Hedgehog ◽  
Adult Brain ◽  
Ganglionic Eminence ◽  
Lateral Ganglionic Eminence ◽  
Expression Characteristic ◽  
Sonic Hedgehog Gene ◽  
Embryonic Structure ◽  
Sequential Induction

The cortex and basal ganglia are the major structures of the adult brain derived from the embryonic telencephalon. Two morphologically distinct regions of the basal ganglia are evident within the mature ventral telencephalon, the globus pallidus medially, and the striatum, which is positioned between the globus pallidus and the cortex. Deletion of the Sonic Hedgehog gene in mice indicates that this secreted signaling molecule is vital for the generation of both these ventral telencephalic regions. Previous experiments showed that Sonic Hedgehog induces differentiation of ventral neurons characteristic of the medial ganglionic eminence, the embryonic structure which gives rise to the globus pallidus. In this paper, we show that later in development, Sonic Hedgehog induces ventral neurons with patterns of gene expression characteristic of the lateral ganglionic eminence. This is the embryonic structure from which the striatum is derived. These results suggest that temporally regulated changes in Sonic Hedgehog responsiveness are integral in the sequential induction of basal telencephalic structures.

scholarly journals The homeobox gene Gsx2 controls the timing of oligodendroglial fate specification in mouse lateral ganglionic eminence progenitors

Development ◽  
2013 ◽  
Vol 140 (11) ◽  
pp. 2289-2298 ◽  
Author(s):  
H. Chapman ◽  
R. R. Waclaw ◽  
Z. Pei ◽  
M. Nakafuku ◽  
K. Campbell
Keyword(s):  
Homeobox Gene ◽  
Ganglionic Eminence ◽  
Fate Specification ◽  
Lateral Ganglionic Eminence

Addition of Lateral Ganglionic Eminence to Rat Mesencephalic Grafts Affects Fiber Outgrowth but Does not Enhance Function

1997 ◽  
Vol 6 (3) ◽  
pp. 277-286 ◽  
Author(s):  
Mia Emgard-Mattson ◽  
Jenny Karlsson ◽  
Naoyuki Nakao ◽  
Patrik Brundin
Keyword(s):  
Dopaminergic Neurons ◽  
Recovery Of Function ◽  
Rotational Behavior ◽  
Specific Marker ◽  
Medial Ganglionic Eminence ◽  
Ganglionic Eminence ◽  
Drug Induced ◽  
Lateral Ganglionic Eminence ◽  
Axonal Fiber ◽  
6 Hydroxydopamine

Addition of embryonic striatal tissue, usually as a combination of the lateral and medial ganglionic eminences, to intrastriatal mesencephalic grafts has previously been reported to enhance recovery of drug-induced rotational behavior in the host and to modify axonal fiber outgrowth from the grafted dopaminergic neurons. This study investigated the effects of adding (cografting) either lateral or medial ganglionic eminence tissue to embryonic mesencephalic grafts implanted intrastriatally, in rats with unilateral 6-hydroxydopamine lesions. The cografts did not exhibit increased survival or cell size of dopaminergic neurons when compared to transplants of mesencephalic tissue alone. Neither did recipients of cografts exhibit any enhancement of graft-induced recovery of function, when tested for drug-induced rotational behavior or forelimb function in the staircase test. However, cografts containing lateral ganglionic eminence displayed patches of dense tyrosine hydroxylase-immunoreactive fibers within the graft tissue. These patches largely coincided with patches in adjacent stained sections, which were rich in immunostaining for the striatal-specific marker dopamine- and cyclic AMP-regulated phosphoprotein-32 (DARPP-32). Such patches were not present in rats receiving cografts containing medial ganglionic eminence or mesencephalic tissue alone. Thus, it seems that the grafted dopaminergic neurons preferentially grow into the areas of the transplants containing lateral ganglionic eminence tissue. In summary, the results suggest that embryonic lateral ganglionic eminence exerts trophic effects on the outgrowth of dopaminergic axons, but does not enhance the behavioral effects of grafted dopaminergic neurons.

Long-term survival of fetal porcine lateral ganglionic eminence cells in the hippocampus of rats

1999 ◽  
Vol 56 (6) ◽  
pp. 581-594 ◽  
Author(s):  
Douglas B. Jacoby ◽  
Charles Lindberg ◽  
Miles G. Cunningham ◽  
Judson Ratliff ◽  
Jonathan Dinsmore
Keyword(s):  
Ganglionic Eminence ◽  
Term Survival ◽  
Long Term Survival ◽  
Lateral Ganglionic Eminence

scholarly journals Cytoarchitecture of the lateral ganglionic eminence and rostral extension of the lateral ventricle in the human fetal brain

2011 ◽  
Vol 519 (6) ◽  
pp. 1165-1180 ◽  
Author(s):  
Hugo Guerrero-Cázares ◽  
Oscar Gonzalez-Perez ◽  
Mario Soriano-Navarro ◽  
Grettel Zamora-Berridi ◽  
José Manuel García-Verdugo ◽  
...  
Keyword(s):  
Lateral Ventricle ◽  
Fetal Brain ◽  
Ganglionic Eminence ◽  
Human Fetal Brain ◽  
Lateral Ganglionic Eminence

scholarly journals The Gli3 Hypomorphic Mutation Pdn Causes Selective Impairment in the Growth, Patterning, and Axon Guidance Capability of the Lateral Ganglionic Eminence

2010 ◽  
Vol 30 (41) ◽  
pp. 13883-13894 ◽  
Author(s):  
D. Magnani ◽  
K. Hasenpusch-Theil ◽  
E. C. Jacobs ◽  
A. T. Campagnoni ◽  
D. J. Price ◽  
...  
Keyword(s):  
Axon Guidance ◽  
Ganglionic Eminence ◽  
Hypomorphic Mutation ◽  
Lateral Ganglionic Eminence ◽  
Selective Impairment
Sign in / Sign up

Export Citation Format

Share Document