scholarly journals The HMG box transcription factors Sox1a and Sox1b specify a new class of glycinergic interneuron in the spinal cord of zebrafish embryos

Development ◽  
2019 ◽  
Vol 146 (4) ◽  
pp. dev172510 ◽  
Author(s):  
Vanessa Gerber ◽  
Lixin Yang ◽  
Masanari Takamiya ◽  
Vanessa Ribes ◽  
Victor Gourain ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Transcription Factors ◽  
Zebrafish Embryos ◽  
Hmg Box ◽  
New Class

scholarly journals Pluripotency factors select gene expression repertoire at Zygotic Genome Activation

2020 ◽  
Author(s):  
Meijiang Gao ◽  
Marina Veil ◽  
Marcus Rosenblatt ◽  
Anna Gebhard ◽  
Helge Hass ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Early Gene ◽  
Zebrafish Embryos ◽  
Fate Specification ◽  
Pluripotency Factors ◽  
Zygotic Transcription ◽  
Simultaneous Loss ◽  
Genome Activation ◽  
Zygotic Genome

AbstractAwakening of zygotic transcription in animal embryos relies on maternal pioneer transcription factors. The interplay of global and specific functions of these proteins remains poorly understood. Here, we analyzed nucleosome positioning, H3K27 acetylation, transcription, and gastrulation rates in zebrafish embryos lacking pluripotency factors Pou5f3 and Sox19b. We show that the bulk transcriptional onset does not require Sox19b and Pou5f3, but is sensitive to their balance. Pou5f3 docks H3K27ac on the enhancers of genes involved in gastrulation and ventral fate specification. Sox19b facilitates Pou5f3 access to one-third of these enhancers. The genes regulating mesendodermal and dorsal fates are primed for activation independently on Pou5f3 and Sox19b. Strikingly, the loss of either factor results in activation of silent enhancers; simultaneous loss of both leads to premature expression of differentiation genes. Our results uncover how independent activities of maternal Pou5f3 and Sox19b add up or antagonize to determine the early gene expression repertoire.

Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos

2021 ◽  
Author(s):  
Tatsuya Yuikawa ◽  
Masaaki Ikeda ◽  
Sachiko Tsuda ◽  
Shinji Saito ◽  
Kyo Yamasu
Keyword(s):  
Spinal Cord ◽  
Transcription Factor ◽  
Zebrafish Embryos

PAX2 is expressed in multiple spinal cord interneurons, including a population of EN1+ interneurons that require PAX6 for their development

Development ◽  
1997 ◽  
Vol 124 (22) ◽  
pp. 4493-4503 ◽  
Author(s):  
J.D. Burrill ◽  
L. Moran ◽  
M.D. Goulding ◽  
H. Saueressig
Keyword(s):  
Spinal Cord ◽  
Transcription Factors ◽  
Ventricular Zone ◽  
Cell Types ◽  
Cell Identity ◽  
Patterning Genes ◽  
Postmitotic Neurons ◽  
Pax Family ◽  
Early Activity

Members of the PAX family of transcription factors are candidates for controlling cell identity in the spinal cord. We have morphologically analyzed cells that express one of these transcription factors, PAX2, demonstrating multiple interneuron cell types express PAX2. Two ventral populations of PAX2-expressing interneurons in the spinal cord are marked by coexpression of the transcription factors, EN1 and EVX1. Interestingly, the expression domains of PAX2, EN1 and EVX1 in postmitotic neurons correlate closely with those of Pax6 and Pax7 in the ventricular zone, implicating these patterning genes in the regulation of PAX2, EN1 and EVX1. We show that one of these patterning genes, Pax6, is required for the correct specification of ventral PAX2+ interneurons that coexpress EN1. These results demonstrate that the early activity of patterning genes in the ventricular zone determines interneuron identity in the spinal cord.

scholarly journals Overexpression of the transcription factors OCT4 and KLF4 improves motor function after spinal cord injury

2020 ◽  
Vol 26 (9) ◽  
pp. 940-951
Author(s):  
Xianpeng Huang ◽  
Chenggui Wang ◽  
Xiaopeng Zhou ◽  
Jingkai Wang ◽  
Kaishun Xia ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Transcription Factors ◽  
Motor Function ◽  
Cord Injury

scholarly journals Notch-regulated oligodendrocyte specification from radial glia in the spinal cord of zebrafish embryos

2008 ◽  
Vol 237 (8) ◽  
pp. 2081-2089 ◽  
Author(s):  
Ho Kim ◽  
Jimann Shin ◽  
Suhyun Kim ◽  
Justin Poling ◽  
Hae-Chul Park ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Radial Glia ◽  
Zebrafish Embryos

scholarly journals Molecular signature of an ancient organizer regulated by Wnt/β-catenin signalling during primary body axis patterning in Hydra

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Puli Chandramouli Reddy ◽  
Akhila Gungi ◽  
Suyog Ubhe ◽  
Saurabh J. Pradhan ◽  
Amol Kolte ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Regulatory Network ◽  
Critical Role ◽  
Ectopic Expression ◽  
Wnt Signalling ◽  
Body Axis ◽  
Molecular Signature ◽  
Zebrafish Embryos ◽  
Primary Body ◽  
Animal Phyla

AbstractWnt/β-catenin signalling has been shown to play a critical role during head organizer formation in Hydra. Here, we characterized the Wnt signalling regulatory network involved in formation of the head organizer. We found that Wnt signalling regulates genes that are important in tissue morphogenesis. We identified that majority of transcription factors (TFs) regulated by Wnt/β-catenin signalling belong to the homeodomain and forkhead families. Silencing of Margin, one of the Wnt regulated homeodomain TFs, results in loss of the ectopic tentacle phenotype typically seen upon activation of Wnt signalling. Furthermore, we show that the Margin promoter is directly bound and regulated by β-catenin. Ectopic expression of Margin in zebrafish embryos results in body axis abnormalities suggesting that Margin plays a role in axis patterning. Our findings suggest that homeobox TFs came under the regulatory umbrella of Wnt/β-catenin signalling presumably resulting in the evolution of primary body axis in animal phyla.

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