scholarly journals Activin A directs striatal projection neuron differentiation of human pluripotent stem cells

Development ◽  
2015 ◽  
Vol 142 (7) ◽  
pp. 1375-1386 ◽  
Author(s):  
C. Arber ◽  
S. V. Precious ◽  
S. Cambray ◽  
J. R. Risner-Janiczek ◽  
C. Kelly ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Projection Neuron ◽  
Activin A ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation

Synergistic effects of FGF-2 and Activin A on early neural differentiation of human pluripotent stem cells

2015 ◽  
Vol 51 (8) ◽  
pp. 769-775 ◽  
Author(s):  
Sumiyo Mimura ◽  
Mika Suga ◽  
Yujung Liu ◽  
Masaki Kinehara ◽  
Kana Yanagihara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Neural Differentiation ◽  
Synergistic Effects ◽  
Activin A ◽  
Human Pluripotent Stem Cells

scholarly journals Stromal factors SDF1α, sFRP1, and VEGFD induce dopaminergic neuron differentiation of human pluripotent stem cells

2012 ◽  
Vol 90 (7) ◽  
pp. 1367-1381 ◽  
Author(s):  
Catherine M. Schwartz ◽  
Tahereh Tavakoli ◽  
Charmaine Jamias ◽  
Sung-Soo Park ◽  
Stuart Maudsley ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Dopaminergic Neuron ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation

scholarly journals Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Daisuke Shimojo ◽  
Kazunari Onodera ◽  
Yukiko Doi-Torii ◽  
Yasuharu Ishihara ◽  
Chinatsu Hattori ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation ◽  
Simple Motor

scholarly journals High-efficiency motor neuron differentiation from human pluripotent stem cells and the function of Islet-1

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Qiuhao Qu ◽  
Dong Li ◽  
Kathleen R. Louis ◽  
Xiangzhen Li ◽  
Hong Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Pluripotent Stem Cells ◽  
High Efficiency ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation ◽  
Islet 1

scholarly journals A role for TGFβ signalling in medium spiny neuron differentiation of human pluripotent stem cells

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Marija Fjodorova ◽  
Zoe Noakes ◽  
Meng Li
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Activin A ◽  
Human Pluripotent Stem Cells ◽  
Medium Spiny Neuron ◽  
Fine Tuning ◽  
Fate Specification ◽  
Differentiation Protocol ◽  
Tgfβ Signalling ◽  
Bmp Signalling

Abstract Activin A and other TGFβ family members have been shown to exhibit a certain degree of promiscuity between their family of receptors. We previously developed an efficient differentiation protocol using Activin A to obtain medium spiny neurons (MSNs) from human pluripotent stem cells (hPSCs). However, the mechanism underlying Activin A-induced MSN fate specification remains largely unknown. Here we begin to tease apart the different components of TGFβ pathways involved in MSN differentiation and demonstrate that Activin A acts exclusively via ALK4/5 receptors to induce MSN progenitor fate during differentiation. Moreover, we show that Alantolactone, an indirect activator of SMAD2/3 signalling, offers an alternative approach to differentiate hPSC-derived forebrain progenitors into MSNs. Further fine tuning of TGFβ pathway by inhibiting BMP signalling with LDN193189 achieves accelerated MSN fate specification. The present study therefore establishes an essential role for TGFβ signalling in human MSN differentiation and provides a fully defined and highly adaptable small molecule-based protocol to obtain MSNs from hPSCs.

scholarly journals Hippo/YAP-mediated rigidity-dependent motor neuron differentiation of human pluripotent stem cells

2014 ◽  
Vol 13 (6) ◽  
pp. 599-604 ◽  
Author(s):  
Yubing Sun ◽  
Koh Meng Aw Yong ◽  
Luis G. Villa-Diaz ◽  
Xiaoli Zhang ◽  
Weiqiang Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation

scholarly journals Nanotopography regulates motor neuron differentiation of human pluripotent stem cells

Nanoscale ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 3556-3565 ◽  
Author(s):  
Weiqiang Chen ◽  
Shuo Han ◽  
Weiyi Qian ◽  
Shinuo Weng ◽  
Haiou Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Motor Neuron ◽  
Pluripotent Stem Cells ◽  
Stem Cell Niche ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation ◽  
Cell Niche

Nanotopographic cues in the stem cell niche regulate the motor neuron differentiation of human pluripotent stem cells.

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