scholarly journals High-efficiency motor neuron differentiation from human pluripotent stem cells and the function of Islet-1

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Qiuhao Qu ◽  
Dong Li ◽  
Kathleen R. Louis ◽  
Xiangzhen Li ◽  
Hong Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Pluripotent Stem Cells ◽  
High Efficiency ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation ◽  
Islet 1

scholarly journals Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Daisuke Shimojo ◽  
Kazunari Onodera ◽  
Yukiko Doi-Torii ◽  
Yasuharu Ishihara ◽  
Chinatsu Hattori ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation ◽  
Simple Motor

scholarly journals Hippo/YAP-mediated rigidity-dependent motor neuron differentiation of human pluripotent stem cells

2014 ◽  
Vol 13 (6) ◽  
pp. 599-604 ◽  
Author(s):  
Yubing Sun ◽  
Koh Meng Aw Yong ◽  
Luis G. Villa-Diaz ◽  
Xiaoli Zhang ◽  
Weiqiang Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation

scholarly journals Nanotopography regulates motor neuron differentiation of human pluripotent stem cells

Nanoscale ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 3556-3565 ◽  
Author(s):  
Weiqiang Chen ◽  
Shuo Han ◽  
Weiyi Qian ◽  
Shinuo Weng ◽  
Haiou Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Motor Neuron ◽  
Pluripotent Stem Cells ◽  
Stem Cell Niche ◽  
Human Pluripotent Stem Cells ◽  
Neuron Differentiation ◽  
Cell Niche

Nanotopographic cues in the stem cell niche regulate the motor neuron differentiation of human pluripotent stem cells.

Effective motor neuron differentiation of hiPSCs on a patch made of crosslinked monolayer gelatin nanofibers

2016 ◽  
Vol 4 (19) ◽  
pp. 3305-3312 ◽  
Author(s):  
Yadong Tang ◽  
Li Liu ◽  
Junjun Li ◽  
Leqian Yu ◽  
Francesco Paolo Ulloa Severino ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Electrode Array ◽  
Plug And Play ◽  
Neuron Differentiation ◽  
Neuron Spike ◽  
Induced Pluripotent ◽  
Multi Electrode Array

A patch made of crosslinked monolayer nanofibers was used for motor neuron differentiation from human induced pluripotent stem cells and plug-and-play with a commercial multi-electrode array for neuron spike recording.

scholarly journals The Opportunities and Challenges regarding Induced Platelets from Human Pluripotent Stem Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Meng-Xue Xu ◽  
Li-Ping Liu ◽  
Yu-Mei Li ◽  
Yun-Wen Zheng
Keyword(s):  
Stem Cells ◽  
Clinical Application ◽  
Pluripotent Stem Cells ◽  
High Efficiency ◽  
Storage Temperature ◽  
Scale Up ◽  
Human Pluripotent Stem Cells ◽  
Scale Production ◽  
Platelet Production ◽  
Platelet Transfusions

As a standard clinical treatment, platelet transfusion has been employed to prevent hemorrhage in patients with thrombocytopenia or platelet dysfunctions. Platelets also show therapeutic potential for aiding liver regeneration and bone healing and regeneration and for treating dermatological conditions. However, the supply of platelets rarely meets the rising clinical demand. Other issues, including short shelf life, strict storage temperature, and allogeneic immunity caused by frequent platelet transfusions, have become serious challenges that require the development of high-yielding alternative sources of platelets. Human pluripotent stem cells (hPSCs) are an unlimited substitution source for regenerative medicine, and patient-derived iPSCs can provide novel research models to explore the pathogenesis of some diseases. Many studies have focused on establishing and modifying protocols for generating functional induced platelets (iPlatelets) from hPSCs. To reach high efficiency production and eliminate the exogenous antigens, media supplements and matrix have been optimized. In addition, the introduction of some critical transgenes, such as c-MYC, BMI1, and BCL-XL, can also significantly increase hPSC-derived platelet production; however, this may pose some safety concerns. Furthermore, many novel culture systems have been developed to scale up the production of iPlatelets, including 2D flow systems, 3D rotary systems, and vertical reciprocal motion liquid culture bioreactors. The development of new gene-editing techniques, such as CRISPR/Cas9, can be used to solve allogeneic immunity of platelet transfusions by knocking out the expression of B2M. Additionally, the functions of iPlatelets were also evaluated from multiple aspects, including but not limited to morphology, structure, cytoskeletal organization, granule content, DNA content, and gene expression. Although the production and functions of iPlatelets are close to meeting clinical application requirements in both quantity and quality, there is still a long way to go for their large-scale production and clinical application. Here, we summarize the diverse methods of platelet production and update the progresses of iPlatelets. Furthermore, we highlight recent advances in our understanding of key transcription factors or molecules that determine the platelet differentiation direction.

scholarly journals Generation of CD34+CD43+ hematopoietic progenitors to induce thymocytes from human pluripotent stem cells

2021 ◽  
Author(s):  
Lea Flippe ◽  
Anne Gaignerie ◽  
Celine Serazin ◽  
Olivier Baron ◽  
Xavier Saulquin ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cells ◽  
Pluripotent Stem Cells ◽  
High Efficiency ◽  
Embryonic Stem ◽  
Human Pluripotent Stem Cells ◽  
Blood Type ◽  
Hematopoietic Stem ◽  
Similar Profile

Immunotherapy using primary T cells has revolutionized medical care in some pathologies in recent years but limitations associated to challenging cell genome edition, insufficient cell number production, the use of only autologous cells and lack of product standardization have limited its uses in the clinic. The alternative use of T cells generated in vitro from human pluripotent stem cells (hPSCs) offers great advantages by providing a self-renewing source of T cells that can be readily genetically modified and facilitate the use of standardized universal off-the-shelf allogeneic cell products and rapid clinic access. However, despite their potential, the feasibility and functionality of T-cells differentiated from hPSCs needs better comprehension before moving to the clinic. In this study, we generated human induced pluripotent stem cells from T-cells (T-iPSCs) allowing preservation of already recombined TCR, with the same properties as human embryonic stem cells (hESCs). Based on these cells, we differentiated with high efficiency hematopoietic progenitor stem cells (HPSCs), capable of self-renewal and differentiation into any cell blood type, and then DN3a thymic progenitors from several T-iPSC lines. To better comprehend differentiation, we analyzed the transcriptomic profiles of the different cell types and demonstrated that HPSCs differentiated from hiPSCs had a very similar profile to cord blood hematopoietic stem cells (HSCs). Furthermore, differentiated T-cell progenitors had a similar profile to thymocytes at the DN3a stage of thymic lymphopoiesis. Therefore, with this approach, we were able to regenerate precursors of therapeutic human T cells to potentially treat a wide number of diseases.

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