orthodenticle activity is required for the development of medial structures in the larval and adult epidermis of Drosophila

Development ◽  
1992 ◽  
Vol 115 (3) ◽  
pp. 801-811 ◽  
Author(s):  
E. Wieschaus ◽  
N. Perrimon ◽  
R. Finkelstein
Keyword(s):  
Pattern Formation ◽  
Gene Product ◽  
Nuclear Protein ◽  
Clonal Analysis ◽  
Precursor Cells ◽  
Cell Fates ◽  
Medial Region ◽  
Genital Discs ◽  
Lateral Structures ◽  
Embryonic Head

Lethal alleles of orthodenticle (= otd) cause abnormalities in the embryonic head that reflect an early role in anterior pattern formation. In addition, otd activity is required for the development of the larval and adult epidermis. Clonal analysis of both viable and lethal alleles shows that the adult requirement for otd is restricted to medial regions of certain discs. When otd activity is reduced or removed, some medial precursor cells produce bristles and cuticle characteristic of more lateral structures. Similar medial defects are observed in the larval epidermis of embryos homozygous for lethal otd alleles. Antibodies to otd recognize a nuclear protein found at high levels in the medial region of the eye antennal discs, the leg discs, the genital discs and along the ventral midline of the ventral epidermis of the embryo. These results suggest that the otd gene product is required to specify medial cell fates in both the larval and adult epidermis.

scholarly journals Organization of the Drosophila head as revealed by the ectopic expression of the Ultrabithorax product

Development ◽  
1991 ◽  
Vol 113 (4) ◽  
pp. 1459-1471 ◽  
Author(s):  
A. Gonzalez-Reyes ◽  
G. Morata
Keyword(s):  
Fusion Gene ◽  
Ectopic Expression ◽  
Precursor Cells ◽  
The Other ◽  
Anterior Region ◽  
Developmental Effects ◽  
Embryonic Head

By using a hsp70-Ubx fusion gene, we have ectopically expressed a Ubx product in the embryonic head primordia and studied the developmental effects on the larval head. We find that after high and persistent levels of Ubx product, the head is replaced by three (C1, C2 and C3) abdominal-like denticle belts. The C2 and C3 belts are the homeotic transformations of parasegments 1 and 2, respectively, while the C1 belt probably derives from the transformation and subsequent fusion of the most anterior procephalic primordia. On the basis of their response to the Ubx product and other arguments, we propose that the larval head is made of two genetically distinct components; one is the procephalon and the anterior region of the mandibular lobe, and the other is part of the parasegmental trunk and includes parasegments 1 and 2. Our results also indicate that most or all the larval head structures derive from precursor cells of ventral origin.

Pattern formation in the limbs of Drosophila: bric a brac is expressed in both a gradient and a wave-like pattern and is required for specification and proper segmentation of the tarsus

Development ◽  
1993 ◽  
Vol 119 (3) ◽  
pp. 799-812 ◽  
Author(s):  
D. Godt ◽  
J.L. Couderc ◽  
S.E. Cramton ◽  
F.A. Laski
Keyword(s):  
Transcription Factors ◽  
Pattern Formation ◽  
Expression Pattern ◽  
Nuclear Protein ◽  
Tarsal Segment ◽  
Conserved Domain ◽  
Bristle Pattern

We have identified the gene bric a brac and show that it is required for pattern formation along the proximal-distal axis of the leg and antenna of Drosophila. In bric a brac mutant legs, the bristle pattern of the three central tarsal segments is transformed towards the pattern of the most proximal tarsal segment. In addition, bric a brac mutant legs and antennae have segmentation defects. bric a brac encodes a nuclear protein that shares a highly conserved domain with two transcription factors from Drosophila. bric a brac function is dosage dependent and is required in a graded manner for the specification of tarsal segments. The graded requirement for bric a brac correlates with its graded expression pattern, suggesting that the concentration of BRIC A BRAC protein specifies segment identity in the tarsus.

Embryonic Precursor Cells That Express Trk Receptors: Induction of Different Cell Fates by NGF, BDNF, NT-3, and CNTF

1997 ◽  
Vol 144 (2) ◽  
pp. 350-360 ◽  
Author(s):  
Mahesh B. Lachyankar ◽  
Peter J. Condon ◽  
Peter J. Quesenberry ◽  
N.Scott Litofsky ◽  
Lawrence D. Recht ◽  
...  
Keyword(s):  
Precursor Cells ◽  
Trk Receptors ◽  
Cell Fates

Assessment of protein gene product 9.5 as a marker of neural crest-derived precursor cells in the developing enteric nervous system

2001 ◽  
Vol 17 (4) ◽  
pp. 304-307 ◽  
Author(s):  
E. L. Sidebotham ◽  
M. N. Woodward ◽  
S. E. Kenny ◽  
D. A. Lloyd ◽  
C. R. Vaillant ◽  
...  
Keyword(s):  
Nervous System ◽  
Neural Crest ◽  
Enteric Nervous System ◽  
Gene Product ◽  
Protein Gene ◽  
Precursor Cells ◽  
Protein Gene Product 9.5 ◽  
Protein Gene Product

scholarly journals Cell fate patterning during C. elegans vulval development

Development ◽  
1993 ◽  
Vol 119 (Supplement) ◽  
pp. 9-18 ◽  
Author(s):  
Russell J. Hill ◽  
Paul W. Sternberg
Keyword(s):  
Cell Fate ◽  
Short Range ◽  
Precursor Cells ◽  
Cell Fates ◽  
Vulval Development ◽  
C Elegans ◽  
Vulval Precursor Cells ◽  
Combined Action ◽  
Anchor Cell ◽  
Inductive Signal

Precursor cells of the vulva of the C. elegans hermaphrodite choose between two vulval cell fates (1° and 2°) and a non-vulval epidermal fate (3°) in response to three intercellular signals. An inductive signal produced by the anchor cell induces the vulval precursors to assume the 1° and 2° vulval fates. This inductive signal is an EGF-like growth factor encoded by the gene lin-3. An inhibitory signal mediated by lin-15, and which may originate from the surrounding epidermis, prevents the vulval precursors from assuming vulval fates in the absence of the inductive signal. A short range lateral signal, which acts through the gene lin-12, regulates the pattern of 1° and 2° fates assumed by the induced vulval precursors. The combined action of the three signals precisely directs the six vulval precursors to adopt a 3° 3° 2° 1° 2 ° 3° pattern of fates. The amount of inductive signal produced by the anchor cell appears to determine the number or vulval precursors that assume vulval fates. The three induced vulval precursors most proximal to the anchor cell are proposed to adopt the 2° 1° 2° pattern of fates in response to a gradient of the inductive signal and also in response to lateral signalling that inhibits adjacent vulval precursor cells from both assuming the 1° fate.

A Clonal Analysis of Secondary Mesenchyme Cell Fates in the Sea Urchin Embryo

1993 ◽  
Vol 160 (1) ◽  
pp. 285-288 ◽  
Author(s):  
Seth W. Ruffins ◽  
Charles A. Ettensohn
Keyword(s):  
Sea Urchin ◽  
Clonal Analysis ◽  
Cell Fates ◽  
Sea Urchin Embryo ◽  
Mesenchyme Cell
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