Positive and negative regulation of cortical cell division during root nodule development in Lotus japonicus is accompanied by auxin response

Development ◽  
2012 ◽  
Vol 139 (21) ◽  
pp. 3997-4006 ◽  
Author(s):  
T. Suzaki ◽  
K. Yano ◽  
M. Ito ◽  
Y. Umehara ◽  
N. Suganuma ◽  
...  
Keyword(s):  
Cell Division ◽  
Root Nodule ◽  
Lotus Japonicus ◽  
Cortical Cell ◽  
Negative Regulation ◽  
Nodule Development ◽  
Auxin Response

scholarly journals Expression Analysis of PIN Genes in Root Tips and Nodules of Lotus japonicus

2019 ◽  
Vol 20 (2) ◽  
pp. 235 ◽  
Author(s):  
Izabela Sańko-Sawczenko ◽  
Dominika Dmitruk ◽  
Barbara Łotocka ◽  
Elżbieta Różańska ◽  
Weronika Czarnocka
Keyword(s):  
Root Nodule ◽  
Root Nodules ◽  
Quantitative Polymerase Chain Reaction ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Vascular Bundles ◽  
Root Tips ◽  
Gus Reporter ◽  
Gene Assay ◽  
Development And Differentiation

Auxins are postulated to be one of the pivotal factors in nodulation. However, their transporters in Lotus japonicus, the model species for the study of the development of determinate-type root nodules, have been scarcely described so far, and thus their role in nodulation has remained unknown. Our research is the first focusing on polar auxin transporters in L. japonicus. We analyzed and compared expression of PINs in 20 days post rhizobial inoculation (dpi) and 54 dpi root nodules of L. japonicus by real-time quantitative polymerase chain reaction (qPCR) along with the histochemical β-glucuronidase (GUS) reporter gene assay in transgenic hairy roots. The results indicate that LjPINs are essential during root nodule development since they are predominantly expressed in the primordia and young, developing nodules. However, along with differentiation, expression levels of several PINs decreased and occurred particularly in the nodule vascular bundles, especially in connection with the root’s stele. Moreover, our study demonstrated the importance of both polar auxin transport and auxin intracellular homeostasis during L. japonicus root nodule development and differentiation.

A shared gene drives lateral root development and root nodule symbiosis pathways in Lotus

Science ◽  
2019 ◽  
Vol 366 (6468) ◽  
pp. 1021-1023 ◽  
Author(s):  
Takashi Soyano ◽  
Yoshikazu Shimoda ◽  
Masayoshi Kawaguchi ◽  
Makoto Hayashi
Keyword(s):  
Root Development ◽  
Lateral Root ◽  
Root Nodule ◽  
Root Nodules ◽  
Lotus Japonicus ◽  
Cortical Cell ◽  
Cortical Cells ◽  
Lateral Root Development ◽  
Lateral Organ ◽  
Nodule Symbiosis

Legumes develop root nodules in symbiosis with nitrogen-fixing rhizobial bacteria. Rhizobia evoke cell division of differentiated cortical cells into root nodule primordia for accommodating bacterial symbionts. In this study, we show that NODULE INCEPTION (NIN), a transcription factor in Lotus japonicus that is essential for initiating cortical cell divisions during nodulation, regulates the gene ASYMMETRIC LEAVES 2-LIKE18/LATERAL ORGAN BOUNDARIES DOMAIN16a (ASL18/LBD16a). Orthologs of ASL18/LBD16a in nonlegume plants are required for lateral root development. Coexpression of ASL18a and the CCAAT box–binding protein Nuclear Factor-Y (NF-Y) subunits, which are also directly targeted by NIN, partially suppressed the nodulation-defective phenotype of L. japonicusdaphne mutants, in which cortical expression of NIN was attenuated. Our results demonstrate that ASL18a and NF-Y together regulate nodule organogenesis. Thus, a lateral root developmental pathway is incorporated downstream of NIN to drive nodule symbiosis.

scholarly journals Spontaneous Root-Nodule Formation in the Model Legume Lotus japonicus: A Novel Class of Mutants Nodulates in the Absence of Rhizobia

2006 ◽  
Vol 19 (4) ◽  
pp. 373-382 ◽  
Author(s):  
Leïla Tirichine ◽  
Euan K. James ◽  
Niels Sandal ◽  
Jens Stougaard
Keyword(s):  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Nodule Formation ◽  
Wild Type ◽  
Model Legume ◽  
Isolation And Characterization ◽  
Mutant Lines ◽  
Spontaneous Nodules

Root-nodule development in legumes is an inducible developmental process initially triggered by perception of lipochitin-oligosaccharide signals secreted by the bacterial microsymbiont. In nature, rhizobial colonization and invasion of the legume root is therefore a prerequisite for formation of nitrogen-fixing root nodules. Here, we report isolation and characterization of chemically induced spontaneously nodulating mutants in a model legume amenable to molecular genetics. Six mutant lines of Lotus japonicus were identified in a screen for spontaneous nodule development under axenic conditions, i.e., in the absence of rhizobia. Spontaneous nodules do not contain rhizobia, bacteroids, or infection threads. Phenotypically, they resemble ineffective white nodules formed by some bacterial mutants on wild-type plants or certain plant mutants inoculated with wild-type Mesorhizobium loti. Spontaneous nodules formed on mutant lines show the ontogeny and characteristic histological features described for rhizobia-induced nodules on wild-type plants. Physiological responses to nitrate and ethylene are also maintained, as elevated levels inhibit spontaneous nodulation. Activation of the nodule developmental program in spontaneous nodules was shown for the early nodulin genes Enod2 and Nin, which are both upregulated in spontaneous nodules as well as in rhizobial nodules. Both monogenic recessive and dominant spontaneous nodule formation (snf) mutations were isolated in this mutant screen, and map positions were determined for three loci. We suggest that future molecular characterization of these mutants will identify key plant determinants involved in regulating nodulation and provide new insight into plant organ development.

scholarly journals The Sym35 Gene Required for Root Nodule Development in Pea Is an Ortholog of Nin from Lotus japonicus

2003 ◽  
Vol 131 (3) ◽  
pp. 1009-1017 ◽  
Author(s):  
Alexey Y. Borisov ◽  
Lene H. Madsen ◽  
Viktor E. Tsyganov ◽  
Yosuke Umehara ◽  
Vera A. Voroshilova ◽  
...  
Keyword(s):  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development

scholarly journals Involvement of auxin distribution in root nodule development of Lotus japonicus

Planta ◽  
2011 ◽  
Vol 234 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Kojiro Takanashi ◽  
Akifumi Sugiyama ◽  
Kazufumi Yazaki
Keyword(s):  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Auxin Distribution

scholarly journals Chitin oligosaccharides can induce cortical cell division in roots of Vicia sativa when delivered by ballistic microtargeting

Development ◽  
1997 ◽  
Vol 124 (23) ◽  
pp. 4887-4895
Author(s):  
H.R. Schlaman ◽  
A.A. Gisel ◽  
N.E. Quaedvlieg ◽  
G.V. Bloemberg ◽  
B.J. Lugtenberg ◽  
...  
Keyword(s):  
Cell Division ◽  
Rhizobium Leguminosarum ◽  
Root Nodule ◽  
Cortical Cell ◽  
Fatty Acyl ◽  
Signal Molecules ◽  
Vicia Sativa ◽  
Fatty Acyl Moiety ◽  
Novel Approach ◽  
Oligosaccharide Moiety

Rhizobia, bacterial symbionts of leguminous plants, produce lipo-chitin oligosaccharide (LCO) signal molecules that can induce nodule organogenesis in the cortex of legume roots in a host-specific way. The multi-unsaturated fatty acyl and the O-acetyl moieties of the LCOs of Rhizobium leguminosarum biovar viciae were shown to be essential for obtaining root nodule induction in Vicia sativa plants. We have used ballistic microtargeting as a novel approach to deliver derivatives of the nodulation signal molecules inside the roots of V. sativa. This method offers the unique ability to introduce soluble compounds into the tissue at a small area. The mitogenic effect of microtargeting of chitin oligosaccharides, including an analysis of the influence of the chain length and modifications, was tested in a qualitative assay. The role of a cell division factor from the root stele, uridine, has also been examined in these experiments. The results show that O-acetylated chitin oligosaccharides can induce root cortical cell divisions when delivered by microtargeting. For this effect it is essential that uridine is co-targeted. The foci of cortical cell division were often similar to root nodule primordia. Anatomical examination also revealed chimeric structures that share characteristics with lateral root and nodule primordia. Our data favour a model in which the oligosaccharide moiety of the rhizobial LCO induces cortical cell division and the fatty acyl moiety plays a role in transport of the LCO into the plant tissue.

scholarly journals The Pea Sym37 Receptor Kinase Gene Controls Infection-Thread Initiation and Nodule Development

2008 ◽  
Vol 21 (12) ◽  
pp. 1600-1608 ◽  
Author(s):  
Vladimir Zhukov ◽  
Simona Radutoiu ◽  
Lene H. Madsen ◽  
Tamara Rychagova ◽  
Evgenia Ovchinnikova ◽  
...  
Keyword(s):  
Middle East ◽  
Rhizobium Leguminosarum ◽  
Lotus Japonicus ◽  
Cortical Cell ◽  
Infection Thread ◽  
Nodule Development ◽  
Phenotypic Characterization ◽  
Receptor Kinase ◽  
Phenotypic Analysis ◽  
Kinase Gene

Phenotypic characterization of pea symbiotic mutants has provided a detailed description of the symbiosis with Rhizobium leguminosarum bv. viciae strains. We show here that two allelic non-nodulating pea mutants, RisNod4 and K24, are affected in the PsSym37 gene, encoding a LysM receptor kinase similar to Lotus japonicus NFR1 and Medicago truncatula LYK3. Phenotypic analysis of RisNod4 and K24 suggests a role for the SYM37 in regulation of infection-thread initiation and nodule development from cortical-cell division foci. We show that RisNod4 plants carrying an L to F substitution in the LysM1 domain display a restrictive symbiotic phenotype comparable to the PsSym2A lines that distinguish ‘European’ and ‘Middle East’ Rhizobium leguminosarum bv. viciae strains. RisNod4 mutants develop nodules only in the presence of a ‘Middle East’ Rhizobium strain producing O-acetylated Nod factors indicating the SYM37 involvement in Nod-factor recognition. Along with the PsSym37, a homologous LysM receptor kinase gene, PsK1, was isolated and characterized. We show that PsK1 and PsSym37 are genetically linked to each other and to the PsSym2 locus. Allelic complementation analyses and sequencing of the extracellular regions of PsSym37 and PsK1 in several ‘European’ and ‘Afghan’ pea cultivars point towards PsK1 as possible candidate for the elusive PsSym2 gene.

scholarly journals A protein phosphatase 2C gene, LjNPP2C1, from Lotus japonicus induced during root nodule development

1999 ◽  
Vol 96 (4) ◽  
pp. 1738-1743 ◽  
Author(s):  
P. Kapranov ◽  
T. J. Jensen ◽  
C. Poulsen ◽  
F. J. de Bruijn ◽  
K. Szczyglowski
Keyword(s):  
Protein Phosphatase ◽  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Protein Phosphatase 2C
Sign in / Sign up

Export Citation Format

Share Document