scholarly journals The Sym35 Gene Required for Root Nodule Development in Pea Is an Ortholog of Nin from Lotus japonicus

2003 ◽  
Vol 131 (3) ◽  
pp. 1009-1017 ◽  
Author(s):  
Alexey Y. Borisov ◽  
Lene H. Madsen ◽  
Viktor E. Tsyganov ◽  
Yosuke Umehara ◽  
Vera A. Voroshilova ◽  
...  
Keyword(s):  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development

scholarly journals Expression Analysis of PIN Genes in Root Tips and Nodules of Lotus japonicus

2019 ◽  
Vol 20 (2) ◽  
pp. 235 ◽  
Author(s):  
Izabela Sańko-Sawczenko ◽  
Dominika Dmitruk ◽  
Barbara Łotocka ◽  
Elżbieta Różańska ◽  
Weronika Czarnocka
Keyword(s):  
Root Nodule ◽  
Root Nodules ◽  
Quantitative Polymerase Chain Reaction ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Vascular Bundles ◽  
Root Tips ◽  
Gus Reporter ◽  
Gene Assay ◽  
Development And Differentiation

Auxins are postulated to be one of the pivotal factors in nodulation. However, their transporters in Lotus japonicus, the model species for the study of the development of determinate-type root nodules, have been scarcely described so far, and thus their role in nodulation has remained unknown. Our research is the first focusing on polar auxin transporters in L. japonicus. We analyzed and compared expression of PINs in 20 days post rhizobial inoculation (dpi) and 54 dpi root nodules of L. japonicus by real-time quantitative polymerase chain reaction (qPCR) along with the histochemical β-glucuronidase (GUS) reporter gene assay in transgenic hairy roots. The results indicate that LjPINs are essential during root nodule development since they are predominantly expressed in the primordia and young, developing nodules. However, along with differentiation, expression levels of several PINs decreased and occurred particularly in the nodule vascular bundles, especially in connection with the root’s stele. Moreover, our study demonstrated the importance of both polar auxin transport and auxin intracellular homeostasis during L. japonicus root nodule development and differentiation.

scholarly journals Spontaneous Root-Nodule Formation in the Model Legume Lotus japonicus: A Novel Class of Mutants Nodulates in the Absence of Rhizobia

2006 ◽  
Vol 19 (4) ◽  
pp. 373-382 ◽  
Author(s):  
Leïla Tirichine ◽  
Euan K. James ◽  
Niels Sandal ◽  
Jens Stougaard
Keyword(s):  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Nodule Formation ◽  
Wild Type ◽  
Model Legume ◽  
Isolation And Characterization ◽  
Mutant Lines ◽  
Spontaneous Nodules

Root-nodule development in legumes is an inducible developmental process initially triggered by perception of lipochitin-oligosaccharide signals secreted by the bacterial microsymbiont. In nature, rhizobial colonization and invasion of the legume root is therefore a prerequisite for formation of nitrogen-fixing root nodules. Here, we report isolation and characterization of chemically induced spontaneously nodulating mutants in a model legume amenable to molecular genetics. Six mutant lines of Lotus japonicus were identified in a screen for spontaneous nodule development under axenic conditions, i.e., in the absence of rhizobia. Spontaneous nodules do not contain rhizobia, bacteroids, or infection threads. Phenotypically, they resemble ineffective white nodules formed by some bacterial mutants on wild-type plants or certain plant mutants inoculated with wild-type Mesorhizobium loti. Spontaneous nodules formed on mutant lines show the ontogeny and characteristic histological features described for rhizobia-induced nodules on wild-type plants. Physiological responses to nitrate and ethylene are also maintained, as elevated levels inhibit spontaneous nodulation. Activation of the nodule developmental program in spontaneous nodules was shown for the early nodulin genes Enod2 and Nin, which are both upregulated in spontaneous nodules as well as in rhizobial nodules. Both monogenic recessive and dominant spontaneous nodule formation (snf) mutations were isolated in this mutant screen, and map positions were determined for three loci. We suggest that future molecular characterization of these mutants will identify key plant determinants involved in regulating nodulation and provide new insight into plant organ development.

scholarly journals Involvement of auxin distribution in root nodule development of Lotus japonicus

Planta ◽  
2011 ◽  
Vol 234 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Kojiro Takanashi ◽  
Akifumi Sugiyama ◽  
Kazufumi Yazaki
Keyword(s):  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Auxin Distribution

scholarly journals A protein phosphatase 2C gene, LjNPP2C1, from Lotus japonicus induced during root nodule development

1999 ◽  
Vol 96 (4) ◽  
pp. 1738-1743 ◽  
Author(s):  
P. Kapranov ◽  
T. J. Jensen ◽  
C. Poulsen ◽  
F. J. de Bruijn ◽  
K. Szczyglowski
Keyword(s):  
Protein Phosphatase ◽  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Protein Phosphatase 2C

scholarly journals NODULE INCEPTION Directly Targets NF-Y Subunit Genes to Regulate Essential Processes of Root Nodule Development in Lotus japonicus

PLoS Genetics ◽  
2013 ◽  
Vol 9 (3) ◽  
pp. e1003352 ◽  
Author(s):  
Takashi Soyano ◽  
Hiroshi Kouchi ◽  
Atsuko Hirota ◽  
Makoto Hayashi
Keyword(s):  
Root Nodule ◽  
Lotus Japonicus ◽  
Nodule Development

Positive and negative regulation of cortical cell division during root nodule development in Lotus japonicus is accompanied by auxin response

Development ◽  
2012 ◽  
Vol 139 (21) ◽  
pp. 3997-4006 ◽  
Author(s):  
T. Suzaki ◽  
K. Yano ◽  
M. Ito ◽  
Y. Umehara ◽  
N. Suganuma ◽  
...  
Keyword(s):  
Cell Division ◽  
Root Nodule ◽  
Lotus Japonicus ◽  
Cortical Cell ◽  
Negative Regulation ◽  
Nodule Development ◽  
Auxin Response

scholarly journals Molecular Characterization of Carbonic Anhydrase Genes in Lotus japonicus and Their Potential Roles in Symbiotic Nitrogen Fixation

2021 ◽  
Vol 22 (15) ◽  
pp. 7766
Author(s):  
Longlong Wang ◽  
Jianjun Liang ◽  
Yu Zhou ◽  
Tao Tian ◽  
Baoli Zhang ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Carbonic Anhydrase ◽  
Root Nodule ◽  
Higher Plants ◽  
Expression Patterns ◽  
Lotus Japonicus ◽  
Nodule Development ◽  
Model Legume ◽  
N Assimilation ◽  
Mature Nodule

Carbonic anhydrase (CA) plays a vital role in photosynthetic tissues of higher plants, whereas its non-photosynthetic role in the symbiotic root nodule was rarely characterized. In this study, 13 CA genes were identified in the model legume Lotus japonicus by comparison with Arabidopsis CA genes. Using qPCR and promoter-reporter fusion methods, three previously identified nodule-enhanced CA genes (LjαCA2, LjαCA6, and LjβCA1) have been further characterized, which exhibit different spatiotemporal expression patterns during nodule development. LjαCA2 was expressed in the central infection zone of the mature nodule, including both infected and uninfected cells. LjαCA6 was restricted to the vascular bundle of the root and nodule. As for LjβCA1, it was expressed in most cell types of nodule primordia but only in peripheral cortical cells and uninfected cells of the mature nodule. Using CRISPR/Cas9 technology, the knockout of LjβCA1 or both LjαCA2 and its homolog, LjαCA1, did not result in abnormal symbiotic phenotype compared with the wild-type plants, suggesting that LjβCA1 or LjαCA1/2 are not essential for the nitrogen fixation under normal symbiotic conditions. Nevertheless, the nodule-enhanced expression patterns and the diverse distributions in different types of cells imply their potential functions during root nodule symbiosis, such as CO2 fixation, N assimilation, and pH regulation, which await further investigations.

Root nodule development: origin, function and regulation of nodulin genes

1992 ◽  
Vol 85 (2) ◽  
pp. 253-265 ◽  
Author(s):  
D. P. S. Verma ◽  
C.-A. Hu ◽  
M. Zhang
Keyword(s):  
Root Nodule ◽  
Nodule Development ◽  
Origin Function ◽  
Nodulin Genes
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