scholarly journals Timed mutation and cell-fate mapping reveal reiterated roles of Tbx1 during embryogenesis, and a crucial function during segmentation of the pharyngeal system via regulation of endoderm expansion

Development ◽  
2005 ◽  
Vol 132 (19) ◽  
pp. 4387-4395 ◽  
Author(s):  
H. Xu
Keyword(s):  
Cell Fate ◽  
Fate Mapping ◽  
Pharyngeal System

scholarly journals Genetic Fate Mapping of Transient Cell Fate Reveals N-Cadherin Activity and Function in Tumor Metastasis

2020 ◽  
Vol 54 (5) ◽  
pp. 593-607.e5 ◽  
Author(s):  
Yan Li ◽  
Zan Lv ◽  
Shaohua Zhang ◽  
Zhuo Wang ◽  
Lingjuan He ◽  
...  
Keyword(s):  
Cell Fate ◽  
Tumor Metastasis ◽  
Fate Mapping ◽  
And Function

Photoactivated Gene Expression for Cell Fate Mapping and Cell Manipulation

2000 ◽  
Vol 2000 (62) ◽  
pp. pl1-pl1 ◽  
Author(s):  
J. Minden ◽  
R. Namba ◽  
J. Mergliano ◽  
S. Cambridge
Keyword(s):  
Gene Expression ◽  
Cell Fate ◽  
Cell Manipulation ◽  
Fate Mapping

scholarly journals Nkx2-5 defines distinct scaffold and recruitment phases during formation of the murine cardiac Purkinje fiber network

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Caroline Choquet ◽  
Robert G. Kelly ◽  
Lucile Miquerol
Keyword(s):  
Transcription Factor ◽  
Electrical Activity ◽  
Cell Fate ◽  
Clonal Analysis ◽  
Conduction System ◽  
Purkinje Fiber ◽  
Fate Mapping ◽  
Fiber Network ◽  
Apply Genetic ◽  
Ventricular Conduction

Abstract The ventricular conduction system coordinates heartbeats by rapid propagation of electrical activity through the Purkinje fiber (PF) network. PFs share common progenitors with contractile cardiomyocytes, yet the mechanisms of segregation and network morphogenesis are poorly understood. Here, we apply genetic fate mapping and temporal clonal analysis to identify murine cardiomyocytes committed to the PF lineage as early as E7.5. We find that a polyclonal PF network emerges by progressive recruitment of conductive precursors to this scaffold from a pool of bipotent progenitors. At late fetal stages, the segregation of conductive cells increases during a phase of rapid recruitment to build the definitive PF network through a non-cell autonomous mechanism. We also show that PF differentiation is impaired in Nkx2-5 haploinsufficient embryos leading to failure to extend the scaffold. In particular, late fetal recruitment fails, resulting in PF hypoplasia and persistence of bipotent progenitors. Our results identify how transcription factor dosage regulates cell fate divergence during distinct phases of PF network morphogenesis.

scholarly journals CellRank for directed single-cell fate mapping

2022 ◽  
Author(s):  
Marius Lange ◽  
Volker Bergen ◽  
Michal Klein ◽  
Manu Setty ◽  
Bernhard Reuter ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Fate ◽  
Normal Development ◽  
Biological Process ◽  
Cellular Reprogramming ◽  
Intermediate Cell ◽  
Fate Mapping ◽  
Directional Information ◽  
Development Data ◽  
Lung Regeneration

AbstractComputational trajectory inference enables the reconstruction of cell state dynamics from single-cell RNA sequencing experiments. However, trajectory inference requires that the direction of a biological process is known, largely limiting its application to differentiating systems in normal development. Here, we present CellRank (https://cellrank.org) for single-cell fate mapping in diverse scenarios, including regeneration, reprogramming and disease, for which direction is unknown. Our approach combines the robustness of trajectory inference with directional information from RNA velocity, taking into account the gradual and stochastic nature of cellular fate decisions, as well as uncertainty in velocity vectors. On pancreas development data, CellRank automatically detects initial, intermediate and terminal populations, predicts fate potentials and visualizes continuous gene expression trends along individual lineages. Applied to lineage-traced cellular reprogramming data, predicted fate probabilities correctly recover reprogramming outcomes. CellRank also predicts a new dedifferentiation trajectory during postinjury lung regeneration, including previously unknown intermediate cell states, which we confirm experimentally.

scholarly journals Genetic tool for fate mapping of Oct4 (Pou5f1)-expressing cells and their progeny past the pluripotency stage

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Andrey A. Kuzmin ◽  
Veronika V. Ermakova ◽  
Sergey A. Sinenko ◽  
Sergey V. Ponomartsev ◽  
Tatiana Y. Starkova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Fate ◽  
Embryonic Stem ◽  
Stop Signal ◽  
Lineage Tracing ◽  
Fate Mapping ◽  
Stage Of Development ◽  
Time Point

Abstract Background Methods based on site-specific recombinases are widely used in studying gene activities in vivo and in vitro. In these studies, constitutively active or inducible variants of these recombinases are expressed under the control of either lineage-specific or ubiquitous promoters. However, there is a need for more advanced schemes that combine these features with possibilities to choose a time point from which lineage tracing starts in an autonomous fashion. For example, the key mammalian germline gatekeeper gene Oct4 (Pou5f1) is expressed in the peri-implantation epiblast which gives rise to all cells within embryos. Thus the above techniques are hardly applicable to Oct4 tracing past the epiblast stage, and the establishment of genetic tools addressing such a limitation is a highly relevant pursuit. Methods The CRISPR/Cas9 tool was used to manipulate the genome of mouse embryonic stem cells (ESCs), and various cell culture technics—to maintain and differentiate ESCs to neural cell, lentivirus-based reprogramming technique—to generate induced pluripotent stem cells (iPSCs). Results In this paper, we have developed a two-component genetic system (referred to as O4S) that allows tracing Oct4 gene activity past the epiblast stage of development. The first component represents a knock-in of an ubiquitous promoter-driven inducible Cre, serving as a stop signal for downstream tdTomato. Upon activation of Cre activity with 4-hydroxytamoxifen (4-OHT) at any given time point, the recombinase excises a stop signal and poses the second component of the system—the FlpO recombinase, knocked into 3’UTR of Oct4, to be expressed upon activation of the latter gene. Oct4-driven expression of FlpO, in turn, triggers the tdTomato expression and thus, permanently marks Oct4+ cells and their progeny. We have validated the O4S system in cultured ESCs and shown that it is capable, for example, to timely capture an activation of Oct4 gene during the reprogramming of somatic cells into iPSCs. Conclusions The developed O4S system can be used to detect Oct4 activation event, both permanent and transient, in somatic cell types outside the germline. The approach can be equally adjusted to other genes, provided the first component of the system is placed under transcriptional control of these genes, thus, making it a valuable tool for cell fate mapping in mice.

scholarly journals Genetic lineage tracing with multiple DNA recombinases: A user's guide for conducting more precise cell fate mapping studies

2020 ◽  
Vol 295 (19) ◽  
pp. 6413-6424 ◽  
Author(s):  
Kuo Liu ◽  
Hengwei Jin ◽  
Bin Zhou
Keyword(s):  
Cell Fate ◽  
Cell Biology ◽  
Lineage Tracing ◽  
Cell Labeling ◽  
Genetic Lineage ◽  
Fate Mapping ◽  
Technical Limitation ◽  
Biological Phenomena ◽  
Recombination System ◽  
Genetic Lineage Tracing

Site-specific recombinases, such as Cre, are a widely used tool for genetic lineage tracing in the fields of developmental biology, neural science, stem cell biology, and regenerative medicine. However, nonspecific cell labeling by some genetic Cre tools remains a technical limitation of this recombination system, which has resulted in data misinterpretation and led to many controversies in the scientific community. In the past decade, to enhance the specificity and precision of genetic targeting, researchers have used two or more orthogonal recombinases simultaneously for labeling cell lineages. Here, we review the history of cell-tracing strategies and then elaborate on the working principle and application of a recently developed dual genetic lineage-tracing approach for cell fate studies. We place an emphasis on discussing the technical strengths and caveats of different methods, with the goal to develop more specific and efficient tracing technologies for cell fate mapping. Our review also provides several examples for how to use different types of DNA recombinase–mediated lineage-tracing strategies to improve the resolution of the cell fate mapping in order to probe and explore cell fate–related biological phenomena in the life sciences.

Sign in / Sign up

Export Citation Format

Share Document