scholarly journals Imaging neural crest cell dynamics during formation of dorsal root ganglia and sympathetic ganglia

Development ◽  
2005 ◽  
Vol 132 (2) ◽  
pp. 235-245 ◽  
Author(s):  
J. C. Kasemeier-Kulesa
Keyword(s):  
Neural Crest ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Neural Crest Cell ◽  
Sympathetic Ganglia ◽  
Cell Dynamics ◽  
Crest Cell

scholarly journals A vital dye analysis of the timing and pathways of avian trunk neural crest cell migration

Development ◽  
1989 ◽  
Vol 106 (4) ◽  
pp. 809-816 ◽  
Author(s):  
G.N. Serbedzija ◽  
M. Bronner-Fraser ◽  
S.E. Fraser
Keyword(s):  
Neural Crest ◽  
Neural Tube ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Sympathetic Ganglia ◽  
Pigment Cells ◽  
Root Cells ◽  
Vital Dye ◽  
Rostral Portion ◽  
Crest Cell

To permit a more detailed analysis of neural crest cell migratory pathways in the chick embryo, neural crest cells were labelled with a nondeleterious membrane intercalating vital dye, DiI. All neural tube cells with endfeet in contact with the lumen, including premigratory neural crest cells, were labelled by pressure injecting a solution of DiI into the lumen of the neural tube. When assayed one to three days later, migrating neural crest cells, motor axons, and ventral root cells were the only cells types external to the neural tube labelled with DiI. During the neural crest cell migratory phase, distinctly labelled cells were found along: (1) a dorsolateral pathway, under the epidermis, as well adjacent to and intercalating through the dermamyotome; and (2) a ventral pathway, through the rostral portion of each sclerotome and around the dorsal aorta as described previously. In contrast to those cells migrating through the sclerotome, labelled cells on the dorsolateral pathway were not segmentally arranged along the rostrocaudal axis. DiI-labelled cells were observed in all truncal neural crest derivatives, including subepidermal presumptive pigment cells, dorsal root ganglia, and sympathetic ganglia. By varying the stage at which the injection was performed, neural crest cell emigration at the level of the wing bud was shown to occur from stage 13 through stage 22. In addition, neural crest cells were found to populate their derivatives in a ventral-to-dorsal order, with the latest emigrating cells migrating exclusively along the dorsolateral pathway.

scholarly journals Visualizing mesoderm and neural crest cell dynamics during chick head morphogenesis

2020 ◽  
Vol 461 (2) ◽  
pp. 184-196 ◽  
Author(s):  
Mary Cathleen McKinney ◽  
Rebecca McLennan ◽  
Rasa Giniunaite ◽  
Ruth E. Baker ◽  
Philip K. Maini ◽  
...  
Keyword(s):  
Neural Crest ◽  
Neural Crest Cell ◽  
Cell Dynamics ◽  
Crest Cell

Colonization of the post-umbilical bowel by cells derived from the sacral neural crest: direct tracing of cell migration using an intercalating probe and a replication-deficient retrovirus

Development ◽  
1991 ◽  
Vol 111 (3) ◽  
pp. 647-655 ◽  
Author(s):  
H.D. Pomeranz ◽  
T.P. Rothman ◽  
M.D. Gershon
Keyword(s):  
Neural Crest ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Sympathetic Ganglia ◽  
Surface Ectoderm ◽  
E Coli ◽  
Infected Cells ◽  
Fluorescent Cells ◽  
Sacral Neural Crest ◽  
Ganglion Of Remak

Experiments were done to test the hypothesis that the avian gut is colonized by cells derived from both vagal and sacral regions of the neural crest. A fluorescent dye, diI (1,1-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate), and a replication-deficient retrovirus (LZ10; Galileo et al. 1990) were employed as tracers. Since LZ10 was constructed with lacZ of E. coli as a reporter gene, infected cells were identified by demonstrating beta-galactosidase immunoreactivity. DiI and LZ10 were injected between the neural tube and surface ectoderm (before the migration of crest cells away from the injection sites) at vagal, truncal (diI only), or sacral axial levels. The bowel was examined 4 days later in order to allow crest-derived cells sufficient time to migrate to the gut. Following injections of either tracer into the vagal crest, labelled cells were found in the gizzard and duodenum. When diI or LZ10 was injected into the sacral crest, labelled cells were seen in the post-umbilical bowel and ganglion of Remak. In the hindgut, marked cells were concentrated in the mesenchyme, just internal to the serosa, and were never observed rostral to the umbilicus. No fluorescent cells were ever found in the bowel following truncal injections of diI, although such cells were observed in sympathetic ganglia. Labelled cells were always found in dorsal root ganglia, no matter which tracer or level of the crest was injected. In embryos injected with LZ10, infected cells in the gut and dorsal root ganglia displayed a neural crest marker (NC-1 immunoreactivity). These observations confirm that the gut is colonized by cells from the sacral as well as the vagal region of the neural crest and that the emigres from the sacral crest are confined to the post-umbilical bowel.

scholarly journals Pathways of trunk neural crest cell migration in the mouse embryo as revealed by vital dye labelling

Development ◽  
1990 ◽  
Vol 108 (4) ◽  
pp. 605-612 ◽  
Author(s):  
G.N. Serbedzija ◽  
S.E. Fraser ◽  
M. Bronner-Fraser
Keyword(s):  
Cell Migration ◽  
Neural Crest ◽  
Neural Tube ◽  
Mouse Embryo ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Sympathetic Ganglia ◽  
Ventral Pathway ◽  
Neural Crest Cell Migration ◽  
Crest Cell

Analysis of neural crest cell migration in the mouse has been difficult due to the lack of reliable cell markers. Recently, we found that injection of DiI into the chick neural tube marks premigratory neural crest cells whose endfeet are in contact with the lumen of the neural tube (Serbedzija et al. Development 106, 809–819 (1989)). In the present study, this technique was applied to study neural crest cell migratory pathways in the trunk of the mouse embryo. Embryos were removed from the mother between the 8th and the 10th days of development and DiI was injected into the lumen of the neural tube. The embryos were then cultured for 12 to 24 h, and analyzed at the level of the forelimb. We observed two predominant pathways of neural crest cell migration: (1) a ventral pathway through the rostral portion of the somite and (2) a dorsolateral pathway between the dermamyotome and the epidermis. Neural crest cells were observed along the dorsolateral pathway throughout the period of migration. The distribution of labelled cells along the ventral pathway suggested that there were two overlapping phases of migration. An early ventrolateral phase began before E9 and ended by E9.5; this pathway consisted of a stream of cells within the rostral sclerotome, adjacent to the dermamyotome, that extended ventrally to the region of the sympathetic ganglia and the dorsal aorta.(ABSTRACT TRUNCATED AT 250 WORDS)

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