scholarly journals Neural Crest Cell Dynamics Revealed by Time-Lapse Video Microscopy of Whole Embryo Chick Explant Cultures

1998 ◽  
Vol 204 (2) ◽  
pp. 327-344 ◽  
Author(s):  
P.M Kulesa ◽  
S.E Fraser
Keyword(s):  
Neural Crest ◽  
Neural Crest Cell ◽  
Time Lapse ◽  
Video Microscopy ◽  
Cell Dynamics ◽  
Explant Cultures ◽  
Embryo Chick ◽  
Crest Cell ◽  
Time Lapse Video

scholarly journals Novel migrating mouse neural crest cell assay system utilizing P0-Cre/EGFP fluorescent time-lapse imaging

2011 ◽  
Vol 11 (1) ◽  
pp. 68 ◽  
Author(s):  
Minoru Kawakami ◽  
Masafumi Umeda ◽  
Naomi Nakagata ◽  
Toru Takeo ◽  
Ken-ichi Yamamura
Keyword(s):  
Neural Crest ◽  
Neural Crest Cell ◽  
Time Lapse ◽  
Assay System ◽  
Cell Assay ◽  
Time Lapse Imaging ◽  
Crest Cell

scholarly journals Visualizing mesoderm and neural crest cell dynamics during chick head morphogenesis

2020 ◽  
Vol 461 (2) ◽  
pp. 184-196 ◽  
Author(s):  
Mary Cathleen McKinney ◽  
Rebecca McLennan ◽  
Rasa Giniunaite ◽  
Ruth E. Baker ◽  
Philip K. Maini ◽  
...  
Keyword(s):  
Neural Crest ◽  
Neural Crest Cell ◽  
Cell Dynamics ◽  
Crest Cell

scholarly journals Head Mesoderm Tissue Growth, Dynamics and Neural Crest Cell Migration

2019 ◽  
Author(s):  
Mary Cathleen McKinney ◽  
Rebecca McLennan ◽  
Rasa Giniunaite ◽  
Ruth E. Baker ◽  
Philip K. Maini ◽  
...  
Keyword(s):  
Cell Migration ◽  
Neural Crest ◽  
Neural Crest Cell ◽  
Time Lapse ◽  
Tissue Growth ◽  
Dynamic Feedback ◽  
Head Mesoderm ◽  
Neural Crest Cell Migration ◽  
Neural Crest Migration ◽  
Crest Cell

ABSTRACTVertebrate head morphogenesis involves orchestrated cell growth and tissue movements of the mesoderm and neural crest to form the distinct craniofacial pattern. To better understand structural birth defects, it is important that we learn how these processes are controlled. Here, we examine this question during chick head morphogenesis using time-lapse imaging, computational modeling, and experiment. We find that head mesodermal cells are inherently dynamic in culture and alter cell behaviors in the presence of either ectoderm or neural crest cells. Mesodermal cells in vivo display large-scale whirling motions that rapidly transition to lateral, directed movements after neural crest cells emerge. Computer model simulations predict distinct changes in neural crest migration as the spatio-temporal growth profile of the mesoderm is varied. BrdU-labeling and photoconversion combined with cell density measurements then reveal non-uniform mesoderm growth in space and time. Chemical inhibition of head mesoderm proliferation or ablation of premigratory neural crest alters mesoderm growth and neural crest migration, implying a dynamic feedback between tissue growth and neural crest cell signaling to confer robustness to the system.Summary StatementDynamic feedback between tissue growth and neural crest cell migration ensures robust neural crest stream formation and head morphogenesis shown by time-lapse microscopy, mathematical modeling and embryo perturbations.

Visualization of enteric neural crest cell migration in SOX10 transgenic mouse gut using time-lapse fluorescence imaging

2011 ◽  
Vol 46 (12) ◽  
pp. 2305-2308 ◽  
Author(s):  
Katsumi Miyahara ◽  
Yoshifumi Kato ◽  
Hiroyuki Koga ◽  
Rafael Dizon ◽  
Geoffrey J. Lane ◽  
...  
Keyword(s):  
Cell Migration ◽  
Neural Crest ◽  
Fluorescence Imaging ◽  
Transgenic Mouse ◽  
Neural Crest Cell ◽  
Time Lapse ◽  
Neural Crest Cell Migration ◽  
Crest Cell

scholarly journals In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest

Development ◽  
2000 ◽  
Vol 127 (13) ◽  
pp. 2843-2852 ◽  
Author(s):  
P. Kulesa ◽  
M. Bronner-Fraser ◽  
S. Fraser
Keyword(s):  
Neural Crest ◽  
Neural Tube ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Time Lapse ◽  
Cell Interactions ◽  
In Ovo ◽  
Relative Contribution ◽  
Crest Cell ◽  
Cell Cell

Previous analyses of single neural crest cell trajectories have suggested important roles for interactions between neural crest cells and the environment, and amongst neural crest cells. To test the relative contribution of intrinsic versus extrinsic information in guiding cells to their appropriate sites, we ablated subpopulations of premigratory chick hindbrain neural crest and followed the remaining neural crest cells over time using a new in ovo imaging technique. Neural crest cell migratory behaviors are dramatically different in ablated compared with unoperated embryos. Deviations from normal migration appear either shortly after cells emerge from the neural tube or en route to the branchial arches, areas where cell-cell interactions typically occur between neural crest cells in normal embryos. Unlike the persistent, directed trajectories in normal embryos, neural crest cells frequently change direction and move somewhat chaotically after ablation. In addition, the migration of neural crest cells in collective chains, commonly observed in normal embryos, was severely disrupted. Hindbrain neural crest cells have the capacity to reroute their migratory pathways and thus compensate for missing neural crest cells after ablation of neighboring populations. Because the alterations in neural crest cell migration are most dramatic in regions that would normally foster cell-cell interactions, the trajectories reported here argue that cell-cell interactions have a key role in the shaping of the neural crest migration.

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