scholarly journals Failure to Detect Measles Virus Ribonucleic Acid in Bone Cells from Patients with Paget’s Disease

2008 ◽  
Vol 93 (4) ◽  
pp. 1398-1401 ◽  
Author(s):  
Brya G. Matthews ◽  
Muhammad A. Afzal ◽  
Philip D. Minor ◽  
Usha Bava ◽  
Karen E. Callon ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Measles Virus ◽  
Bone Cells ◽  
Bone Marrow Cells ◽  
Subacute Sclerosing Panencephalitis ◽  
Paget’S Disease ◽  
Pcr Amplification ◽  
Positive Control ◽  
Paget's Disease ◽  
Marrow Cells

Abstract Background: Paget’s disease is a condition of focal accelerated bone turnover. Electron-microscopy investigations of osteoclasts from pagetic lesions have identified nuclear inclusion bodies that have a similar appearance to viral nucleocapsid particles. Subsequently, RNA from several paramyxoviruses has been detected in pagetic tissue, and it was suggested that these viruses, in particular measles, might play a role in the etiology of Paget’s disease. We have tested for measles virus sequences in osteoblasts and bone marrow cells collected from pagetic lesions and healthy bone. Methods: Bone and bone marrow samples were taken from Paget’s patients and control subjects, and cells were cultured from each of these tissues. RNA was extracted from 13 osteoblast cultures and 13 cultures of bone marrow cells derived from pagetic lesions, and from 26 and 23 control osteoblast and bone marrow cultures, respectively. These samples were sourced from 22 patients with Paget’s disease and 31 controls. RT-PCR-nested PCR amplification was used for the detection of the genes for the measles nucleocapsid and matrix proteins. Results: Measles virus sequences were not detected in any of the pagetic or control samples. However, measles virus sequences were identified in samples of a measles virus culture isolate included as a positive control, and in a brain sample from a patient with subacute sclerosing panencephalitis, a condition associated with chronic measles infection. Conclusion: The results of the study do not support the hypothesis that measles virus plays a role in the pathogenesis of Paget’s disease.

scholarly journals Enhanced RANK ligand expression and responsivity of bone marrow cells in Paget’s disease of bone

2000 ◽  
Vol 105 (12) ◽  
pp. 1833-1838 ◽  
Author(s):  
Cheikh Menaa ◽  
Sakamuri V. Reddy ◽  
Noriyoshi Kurihara ◽  
Hidefumi Maeda ◽  
Dirk Anderson ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Paget’S Disease ◽  
Paget's Disease ◽  
Paget’S Disease Of Bone ◽  
Rank Ligand ◽  
Paget's Disease Of Bone ◽  
Ligand Expression ◽  
Marrow Cells

scholarly journals Absence of Somatic SQSTM1 Mutations in Paget’s Disease of Bone

2009 ◽  
Vol 94 (2) ◽  
pp. 691-694 ◽  
Author(s):  
Brya G. Matthews ◽  
Dorit Naot ◽  
Usha Bava ◽  
Karen E. Callon ◽  
Rocco P. Pitto ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Environmental Factors ◽  
Somatic Mutations ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
Paget’S Disease ◽  
Paget's Disease ◽  
Allelic Discrimination ◽  
Sporadic Cases ◽  
Genetic And Environmental Factors

Abstract Background: Paget’s disease is a common focal bone disorder that appears to be caused by a combination of genetic and environmental factors. Mutations in the SQSTM1 gene are found in about one third of families with Paget’s disease and 8% of sporadic cases. Other potential loci linked to the disease have also been identified, and a number of environmental factors have been suggested to be involved in the disease. However, the focal nature of Paget’s is still unexplained. Therefore, we examined the possibility that somatic mutations in the SQSTM1 gene are present in the local lesions, using RNA collected from primary osteoblast and bone marrow cell cultures of patients with this condition. Methods: SQSTM1 was sequenced, and allelic discrimination for the common P392L mutation was performed in cDNA samples from 14 osteoblast cultures and from 14 cultures of bone marrow cells. Results: In these 28 samples drawn from 23 patients, the wild-type sequence of SQSTM1 was found in all but one marrow sample, which was heterozygous for the P392L mutation. DNA from peripheral blood in this subject had an identical sequence of SQSTM1, indicating that this was a germline mutation. Conclusion: We conclude that somatic mutations for SQSTM1 are not commonly present in Paget’s disease.

scholarly journals Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
Sahdeo Prasad ◽  
Smita Srivastava ◽  
Madhulika Singh ◽  
Yogeshwer Shukla
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Positive Control ◽  
Vehicle Group ◽  
Control Group ◽  
Mouse Bone Marrow ◽  
Human Beings ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Marrow Cells

Glyphosate (N-(phosphonomethyl) glycine,C3H8NO5P), a herbicide, used to control unwanted annual and perennial plants all over the world. Nevertheless, occupational and environmental exposure to pesticides can pose a threat to nontarget species including human beings. Therefore, in the present study, genotoxic effects of the herbicide glyphosate were analyzed by measuring chromosomal aberrations (CAs) and micronuclei (MN) in bone marrow cells of Swiss albino mice. A single dose of glyphosate was given intraperitoneally (i.p) to the animals at a concentration of 25 and 50 mg/kg b.wt. Animals of positive control group were injectedi.p. benzo(a)pyrene (100 mg/kg b.wt., once only), whereas, animals of control (vehicle) group were injectedi.p. dimethyl sulfoxide (0.2mL). Animals from all the groups were sacrificed at sampling times of 24, 48, and 72 hours and their bone marrow was analyzed for cytogenetic and chromosomal damage. Glyphosate treatment significantly increases CAs and MN induction at both treatments and time compared with the vehicle control (P<.05). The cytotoxic effects of glyphosate were also evident, as observed by significant decrease in mitotic index (MI). The present results indicate that glyphosate is clastogenic and cytotoxic to mouse bone marrow.

scholarly journals The combination of lipopolysaccharide and D-galactosamine administration show positive genotoxic effect in mice liver

2020 ◽  
Author(s):  
Wenjing Dong ◽  
Erqun Song ◽  
Yang Song
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Bone Marrow Cells ◽  
Histopathological Examination ◽  
Chronic Administration ◽  
Positive Control ◽  
Genotoxic Effect ◽  
Acute Administration ◽  
Scge Assay ◽  
Marrow Cells

AbstractLipopolysaccharide (LPS)/D-galactosamine (D-GalN) co-administration induced acute liver injury (ALI) and hepatic fibrosis have been extensively studied. However, whether LPS/D-GalN show genotoxic effect is current unknown. Male mice were divided into eight groups and each group contain eight animals. For the acute administration of LPS/D-GalN, the mice were given a single intraperitoneal (i.p.) injection of LPS/D-GalN (25 μg/kg + 250 mg/kg, 25 μg/kg + 500 mg/kg, 50 μg/kg + 500 mg/kg body weight) for 6 h, respectively. The chronic administration was conduct by the i.p. injection of LPS/D-GalN (10 μg/kg + 100 mg/kg) every other day for 8 weeks. Saline solution (0.9%) and cyclophosphamide (CTX) (50 mg/kg body weight) injection were used as negative and positive control, respectively. Using single cell gel electrophoresis (SCGE) assay, we found that the acute administration of LPS/D-GalN induces severe DNA damage in mice hepatic cells, but not in brain, sperm and bone marrow cells, implied the genotoxicity of LPS/D-GalN. Interestingly, the chronic treatment of LPS/D-GalN causes significant genotoxic effect in both hepatic and brain cells, but not sperm and bone marrow cells. Histopathological examination in liver and brain section consistent with SCGE results, accordingly. Our study, for the first time, reported the genotoxic potential of LPS/D-GalN co-administration. In addition, LPS/D-GalN administration may serve as an experimental model for further genotoxic study.

Linking bone cells, aging, and oxidative stress: Osteoblasts, osteoclasts, osteocytes, and bone marrow cells

Aging ◽  
2020 ◽  
pp. 61-71
Author(s):  
Juan A. Ardura ◽  
Luis Álvarez-Carrión ◽  
Arancha R. Gortázar ◽  
Verónica Alonso
Keyword(s):  
Oxidative Stress ◽  
Bone Marrow ◽  
Bone Cells ◽  
Bone Marrow Cells ◽  
And Oxidative Stress ◽  
Marrow Cells

Polymer Surface Modification Causes Change in Phenotypic Expression of Primary Bone Cells

1991 ◽  
Vol 252 ◽  
Author(s):  
Sean A. F. Peel ◽  
R. N. Sodhi ◽  
Tran Minh Duc ◽  
John E. Davies
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Photoelectron Spectroscopy ◽  
Bone Cells ◽  
Bone Marrow Cells ◽  
Polymer Surface ◽  
Phenotypic Expression ◽  
Force Microscopy ◽  
Matrix Deposition ◽  
Marrow Cells

ABSTRACTPrimary rat bone marrow cells were cultured on bacteriological grade polystyrene dishes which had been treated in selected areas with concentrated sulphuric acid. X-ray photoelectron spectroscopy, angle-resolved and imaging modes, and atomic force microscopy showed that the acid treatment brought about both chemical and topographical changes in the substratum surface. While the bone marrow cells attached to both treated and untreated areas of the surfaces of the dishes, the distribution of early mineralized extracellular matrix in these areas was reproducibily different. Thus, using extracellular matrix deposition as a marker, modification of the polymer surface resulted in the cells adhering to treated and untreated areas exhibiting different phenotypes.

Immunohistochemical Evidence of Measles Virus Antigens in Active Otosclerosis

1989 ◽  
Vol 101 (4) ◽  
pp. 415-421 ◽  
Author(s):  
Michael J. McKenna ◽  
Barbara G. Mills
Keyword(s):  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Paget’S Disease ◽  
Paget's Disease ◽  
Viral Origin ◽  
The Central Nervous System ◽  
Virus Antigens ◽  
Syncytial Virus ◽  
Immunohistochemical Evidence ◽  
Intensive Investigation

Despite intensive investigation, the cause of otosclerosis remains uncertain. Recent studies of Paget's disease of bone have revealed a possible viral origin. Because of similarities between otosclerosis and Paget's disease, we have pursued investigation of a possible viral cause of otosclerosis. Four temporal bone specimens from patients with otosclerosis, processed for immunohistochemistry, demonstrated positive specific reactivity with monoclonal antibodies to measles virus antigens using the indirect immunofluorescent and immunoperoxidase techniques. Reactivity was most intense in active foci. Reactivity in the peroxidase assay was also observed in areas of active otosclerosis with application of primary antisera from patients with subacute sclerosing panencephalitis, a disorder of the central nervous system in which a defective measles virus has been isolated. Other related paramyxoviruses, including mumps and respiratory syncytial virus, were negative, as were negative controls.

Sign in / Sign up

Export Citation Format

Share Document