Polymer Surface Modification Causes Change in Phenotypic Expression of Primary Bone Cells

1991 ◽  
Vol 252 ◽  
Author(s):  
Sean A. F. Peel ◽  
R. N. Sodhi ◽  
Tran Minh Duc ◽  
John E. Davies
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Photoelectron Spectroscopy ◽  
Bone Cells ◽  
Bone Marrow Cells ◽  
Polymer Surface ◽  
Phenotypic Expression ◽  
Force Microscopy ◽  
Matrix Deposition ◽  
Marrow Cells

ABSTRACTPrimary rat bone marrow cells were cultured on bacteriological grade polystyrene dishes which had been treated in selected areas with concentrated sulphuric acid. X-ray photoelectron spectroscopy, angle-resolved and imaging modes, and atomic force microscopy showed that the acid treatment brought about both chemical and topographical changes in the substratum surface. While the bone marrow cells attached to both treated and untreated areas of the surfaces of the dishes, the distribution of early mineralized extracellular matrix in these areas was reproducibily different. Thus, using extracellular matrix deposition as a marker, modification of the polymer surface resulted in the cells adhering to treated and untreated areas exhibiting different phenotypes.

Myocardial Assistance by Grafting a New Bioartificial Upgraded Myocardium (MAGNUM Clinical Trial): One Year Follow-Up

2007 ◽  
Vol 16 (9) ◽  
pp. 927-934 ◽  
Author(s):  
Juan C. Chachques ◽  
Jorge C. Trainini ◽  
Noemi Lago ◽  
Osvaldo H. Masoli ◽  
Jose L. Barisani ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Cell Therapy ◽  
Bone Marrow Cells ◽  
Type I ◽  
Intramyocardial Injection ◽  
Cardiac Wall ◽  
Mononuclear Bone Marrow Cells ◽  
Marrow Cells

Cell transplantation for the regeneration of ischemic myocardium is limited by poor graft viability and low cell retention. In ischemic cardiomyopathy the extracellular matrix is deeply altered; therefore, it could be important to associate a procedure aiming at regenerating myocardial cells and restoring the extracellular matrix function. We evaluated intrainfarct cell therapy associated with a cell-seeded collagen scaffold grafted onto infarcted ventricles. In 15 patients (aged 54.2 ± 3.8 years) presenting LV postischemic myocardial scars and with indication for a single OP-CABG, autologous mononuclear bone marrow cells (BMC) were implanted during surgery in the scar. A 3D collagen type I matrix seeded with the same number of BMC was added on top of the scarred area. There was no mortality and no related adverse events (follow-up 15 ± 4.2 months). NYHA FC improved from 2.3 ± 0.5 to 1.4 ± 0.3 (p = 0.005). LV end-diastolic volume evolved from 142 ± 24 to 117 ± 21 ml (p = 0.03), and LV filling deceleration time improved from 162 ± 7 to 196 ± 8 ms (p = 0.01). Scar area thickness progressed from 6 ± 1.4 to 9 ± 1.5 mm (p = 0.005). EF improved from 25 ± 7% to 33 ± 5% (p = 0.04). Simultaneous intramyocardial injection of mononuclear bone marrow cells and fixation of a BMC-seeded matrix onto the epicardium is feasible and safe. The cell-seeded collagen matrix seems to increase the thickness of the infarct scar with viable tissues and helps to normalize cardiac wall stress in injured regions, thus limiting ventricular remodeling and improving diastolic function. Patients' improvements cannot be conclusively related to the cells and matrix due to the association of CABG. Cardiac tissue engineering seems to extend the indications and benefits of stem cell therapy in cardiology, becoming a promising way for the creation of a “bioartificial myocardium.” Efficacy and safety of this approach should be evaluated in a large randomized controlled trial.

scholarly journals Failure to Detect Measles Virus Ribonucleic Acid in Bone Cells from Patients with Paget’s Disease

2008 ◽  
Vol 93 (4) ◽  
pp. 1398-1401 ◽  
Author(s):  
Brya G. Matthews ◽  
Muhammad A. Afzal ◽  
Philip D. Minor ◽  
Usha Bava ◽  
Karen E. Callon ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Measles Virus ◽  
Bone Cells ◽  
Bone Marrow Cells ◽  
Subacute Sclerosing Panencephalitis ◽  
Paget’S Disease ◽  
Pcr Amplification ◽  
Positive Control ◽  
Paget's Disease ◽  
Marrow Cells

Abstract Background: Paget’s disease is a condition of focal accelerated bone turnover. Electron-microscopy investigations of osteoclasts from pagetic lesions have identified nuclear inclusion bodies that have a similar appearance to viral nucleocapsid particles. Subsequently, RNA from several paramyxoviruses has been detected in pagetic tissue, and it was suggested that these viruses, in particular measles, might play a role in the etiology of Paget’s disease. We have tested for measles virus sequences in osteoblasts and bone marrow cells collected from pagetic lesions and healthy bone. Methods: Bone and bone marrow samples were taken from Paget’s patients and control subjects, and cells were cultured from each of these tissues. RNA was extracted from 13 osteoblast cultures and 13 cultures of bone marrow cells derived from pagetic lesions, and from 26 and 23 control osteoblast and bone marrow cultures, respectively. These samples were sourced from 22 patients with Paget’s disease and 31 controls. RT-PCR-nested PCR amplification was used for the detection of the genes for the measles nucleocapsid and matrix proteins. Results: Measles virus sequences were not detected in any of the pagetic or control samples. However, measles virus sequences were identified in samples of a measles virus culture isolate included as a positive control, and in a brain sample from a patient with subacute sclerosing panencephalitis, a condition associated with chronic measles infection. Conclusion: The results of the study do not support the hypothesis that measles virus plays a role in the pathogenesis of Paget’s disease.

Improvement of Chronic Wound Healing by Pre-activated Bone Marrow Cells with Sodium Nitroprusside in Rabbits

Drug Research ◽  
2021 ◽  
Author(s):  
Nazira Fatima ◽  
Muhammad Saleem ◽  
Umar Shahbaz
Keyword(s):  
Oxidative Stress ◽  
Bone Marrow ◽  
Wound Healing ◽  
Chronic Wound ◽  
Bone Marrow Cells ◽  
Matrix Deposition ◽  
Cell Based Therapy ◽  
Diabetic Rabbits ◽  
Marrow Cells

Abstract Aim of study This study investigated whether pre-activated bone marrow cells with sodium nitro prusside have effectiveness in the inhibition of diabetic wound healing in diabetic rabbits. In diabetic skin disorders and conditions involved redox state disturbances. The aim was to determine the effect of two minimum dosages of sodium nitro prusside, and its’ potential with bone marrow cells for chronic wound healing in-vivo. Methods Full-thickness skin dorsal wounds were created on diabetic rabbits. The effects of two minimum concentrations of sodium nitro prusside solution with bone marrow cells on wound healing were studied. The useful combination of sodium nitro prusside with bone marrow cells on wound repair may be attributed to its functional influences on inflammation, angiogenesis, cell proliferation, matrix deposition, and remodeling. Results The in-vivo experiments confirmed that pre-activated bone marrow cells contributed to wound healing by alleviating oxidative stress, increasing proliferation and migration, decreasing apoptosis. In histological results, improved collagen deposition, enhanced re-epithelization, angiogenesis, and decreased inflammatory infiltration were also detected in wound biopsies. Conclusions For the treatment of chronic wounds, cell-based therapy was an attractive approach. Bone marrow cells have a low ability to differentiate various types of cells or late healing without pretreatment. So it was needed to increase their potency of differentiation. The transplantation of pretreated bone marrow cells with a prime quantity of sodium nitro prusside solution improved chronic wound healing with a greater level of growth factors and a minimum level of oxidative stress.

The in vitro formation of a calcified extracellular matrix on biomaterials by rat bone marrow cells

Bone ◽  
1992 ◽  
Vol 13 (1) ◽  
pp. 99-99
Author(s):  
J.D. de Bruijn ◽  
C.P.A.T. Klein ◽  
K. de Groot ◽  
J.E. Davies ◽  
C.A. van Blitterswijk
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Bone Marrow Cells ◽  
Rat Bone ◽  
Marrow Cells

Linking bone cells, aging, and oxidative stress: Osteoblasts, osteoclasts, osteocytes, and bone marrow cells

Aging ◽  
2020 ◽  
pp. 61-71
Author(s):  
Juan A. Ardura ◽  
Luis Álvarez-Carrión ◽  
Arancha R. Gortázar ◽  
Verónica Alonso
Keyword(s):  
Oxidative Stress ◽  
Bone Marrow ◽  
Bone Cells ◽  
Bone Marrow Cells ◽  
And Oxidative Stress ◽  
Marrow Cells

Differentiation of rat bone marrow cells cultured on artificial basement membrane containing extracellular matrix into a liver cell lineage

2005 ◽  
Vol 43 (1) ◽  
pp. 110-116 ◽  
Author(s):  
Kazuo Okumoto ◽  
Takafumi Saito ◽  
Etsuko Hattori ◽  
Jun-itsu Ito ◽  
Akihiko Suzuki ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Basement Membrane ◽  
Liver Cell ◽  
Bone Marrow Cells ◽  
Cell Lineage ◽  
Cells Cultured ◽  
Rat Bone ◽  
Marrow Cells

CABG and intramyocardial delivery of CD133+ bone marrow cells: 3-years follow-up on safety and efficacy

2006 ◽  
Vol 54 (S 1) ◽  
Author(s):  
C Stamm ◽  
YH Choi ◽  
A Liebold ◽  
HD Kleine ◽  
S Dunkelmann ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Safety And Efficacy ◽  
Intramyocardial Delivery ◽  
Marrow Cells
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