Chromatography with Liquid Mobile Phases

2021 ◽  
pp. 683-750
Author(s):  
James W. Robinson ◽  
Eileen M. Skelly Frame ◽  
George M. Frame
Keyword(s):  
Mobile Phases

Theoretical Interpretation of High-Performance Chromatographic Data for a Heterogeneous Series of Compounds

1992 ◽  
Vol 57 (1) ◽  
pp. 33-45
Author(s):  
Vladimír Jakuš
Keyword(s):  
Experimental Data ◽  
High Performance ◽  
Theoretical Interpretation ◽  
Retention Data ◽  
Theoretical Evaluation ◽  
New Approach ◽  
Free Energy Of Solvation ◽  
Rp Hplc ◽  
Mobile Phases ◽  
Reversed Phases

A new approach to theoretical evaluation of the Gibbs free energy of solvation was applied for estimation of retention data in high-performance liquid chromatography on reversed phases (RP-HPLC). Simple and improved models of stationary and mobile phases in RP-HPLC were employed. Statistically significant correlations between the calculated and experimental data were obtained for a heterogeneous series of twelve compounds.

scholarly journals Development and Validation of Stability-Indicating RP-HPLC Method for the Estimation of Lenalidomide and its Impurities in Oral Solid Dosage Form

2019 ◽  
Vol 35 (1) ◽  
pp. 140-149 ◽  
Author(s):  
Somana Siva Prasad ◽  
G. V. Krishna Mohan ◽  
A. Naga Babu
Keyword(s):  
Limit Of Detection ◽  
Degradation Products ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Stability Robustness ◽  
Rp Hplc ◽  
Mobile Phases ◽  
Limit Of Quantitation ◽  
Successful Separation ◽  
Quality By Design Approach

In this study, a novel, simple and precise RP-HPLC method has been developed for the quantitative analysis of Lenalidomide (LLM) in pharmaceutical formulations using analytical quality by design approach. An X-bridge-C18 column (150 mm × 4.6 mm × 3.5 µ) with mobile phases containing a Potassium dihydrogen orthophosphate anhydrous buffer and methanol in the ratio of (90:10 v/v) and (35:65 v/v) are used for the estimation of LLM and its degradation products. The flow rate of 0.8 mL/min is maintained and all degradation studies are performed at 210 nm using photodiode array (PDA) detector. Method Validation is carried out according to International Council for Harmonisation (ICH) guidelines and the parameters namely; precision, accuracy, specificity, stability, robustness, linearity, limit of quantitation (LOQ) and limit of detection (LOD) are evaluated. The present developed RP-HPLC method shows the purity angle of peaks is less than their threshold angle, signifying that it to be suitable for stability studies. Hence, the developed method can be used for the successful separation of LLM and its impurities in the pharmaceutical dosage formulations.

Instability of hexane-acetonitrile mobile phases used for the chromatographic analysis of triacylglycerides

2008 ◽  
Vol 31 (6-7) ◽  
pp. NA-NA
Author(s):  
Robert G. Harfmann ◽  
Samir Julka ◽  
Hernan J. Cortes
Keyword(s):  
Chromatographic Analysis ◽  
Mobile Phases

scholarly journals Redistribution of Zn towards light-density fractions and potentially mobile phases in a long-term manure-amended clayey soil

Geoderma ◽  
2021 ◽  
Vol 394 ◽  
pp. 115044
Author(s):  
Thiago A. Formentini ◽  
Isabelle Basile-Doelsch ◽  
Samuel Legros ◽  
Daniel Borschneck ◽  
Julia S. Venzon ◽  
...  
Keyword(s):  
Clayey Soil ◽  
Density Fractions ◽  
Mobile Phases ◽  
Light Density
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