Effect of the composition of two-component mobile phases on the separation of selected porphyrins by partition thin-layer chromatography

2007 ◽  
Vol 20 (4) ◽  
pp. 259-260 ◽  
Author(s):  
Marzena Podgórna
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phases ◽  
Two Component ◽  
Layer Chromatography ◽  
Effect Of The Composition

scholarly journals Normal and reversed phase thin-layer chromatography of new 16, 17-secoestrone derivatives

2003 ◽  
Vol 68 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Marijana Acanski ◽  
Suzana Jovanovic-Santa ◽  
Lidija Jevric
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Reversed Phase ◽  
Retention Behavior ◽  
Mobile Phases ◽  
Layer Chromatography

The retention behavior and separation ability of a series of new 16,17-secoestrone derivatives has been studied on silica gel, alumina and C-18 silica gel layers with non-aqueous and aqueous-organic mobile phases. The retention behavior and separation ability are discussed in terms of the nature of the solute, eluent and stationary phase.

scholarly journals Reversed-phase thin-layer chromatography behavior of aldopentose derivatives

2012 ◽  
Vol 66 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Dragana Livaja-Popovic ◽  
Eva Loncar ◽  
Lidija Jevric ◽  
Radomir Malbasa
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Chromatographic Behavior ◽  
Log P ◽  
Retention Factors ◽  
Mobile Phases ◽  
Acetone Water ◽  
Layer Chromatography

Quantitative structure-retention relationships (QSRR) have been used to study the chromatographic behavior of some aldopentose. The behavior of aldopentose derivatives was investigated by means of the reversed-phase thin-layer chromatography (RP TLC) on the silica gel impregnated with paraffin oil stationary phases. Binary mixtures of methanol-water, acetone-water and dioxane-water were used as mobile phases. Retention factors, RM0, corresponding to zero percent organic modifier in the aqueous mobile phase was determined. Lipophilicity C0 was calculated as the ratio of the intercept and slope values. There was satisfactory correlation between them and log P values calculated using different theoretical procedures. Some of these correlations offer very good predicting models, which are important for a better understanding of the relationships between chemical structure and retention. The study showed that the hydrophobic parameters RM0 and C0 can be used as a measures of lipophilicity of investigated compounds.

The Identification of Rubber Compounding Ingredients by Using Thin Layer Chromatography

1983 ◽  
Vol 56 (5) ◽  
pp. 1080-1095 ◽  
Author(s):  
B. J. Gedeon ◽  
T. Chu ◽  
S. Copeland
Keyword(s):  
Molecular Weight ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Easy Method ◽  
Mobile Phases ◽  
Rf Values ◽  
Visualization Systems ◽  
Layer Chromatography

Abstract TLC is a quick, easy method to the identification of rubber compounding ingredients. Both silica gel absorbent and C18 absorbent are suitable for these identifications. With the mobile phases used in this study, a better separation of antioxidants is possible using silica gel absorbent. Work should be continued on finding suitable mobile phases for the C18 absorbent, since the Rf values are more reproducible. For those separations that require a separation of compounds of varying molecular weight, the C18 absorbent is superior to silica gel absorbent. The visualization systems that have been developed for use with silica gel absorbent are suitable for use with C18 absorbent. These systems give the same colors for either absorbent.

Electroosmotic Thin Layer Chromatography with Low-Volatile Mobile Phases

2004 ◽  
Vol 398 (1-3) ◽  
pp. 223-225 ◽  
Author(s):  
V. G. Berezkin ◽  
A. O. Balushkin ◽  
B. V. Tyaglov ◽  
E. F. Litvin
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phases ◽  
Layer Chromatography

scholarly journals Identification and Determination of Oxytetracycline, Tiamulin, Lincomycin, and Spectinomycin in Veterinary Preparations by Thin-Layer Chromatography/Densitometry

2000 ◽  
Vol 83 (6) ◽  
pp. 1502-1506 ◽  
Author(s):  
Jan Krzek ◽  
Anna Kwiecień ◽  
Małgorzata Starek ◽  
Anna Kierszniewska ◽  
Włodzimierz Rzeszutko
Keyword(s):  
Quantitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Citric Acid Solution ◽  
Active Constituents ◽  
Mobile Phases ◽  
Rf Values ◽  
Layer Chromatography ◽  
Small Confidence Interval

Abstract A thin-layer chromatographic/densitometric method was developed for the identification and quantitation of oxytetracycline, tiamulin, lincomycin, and spectinomycin in veterinary preparations. Silica gel-coated thin layer chromatography plates and 2 mobile phases were used to separate these constituents. The appropriate compositions of the suitable mobile phases were established: 10% citric acid solution–n-hexane–ethanol (80 + 1 + 1, v/v) and n–butanol–ethanol–chloroform– 25% ammonia (4 + 5 + 2 + 5, v/v). Along with Rf values and spot colors, direct UV and visual densitometric measurements were used for identification. Similar measuring ranges were used for quantitative analysis to obtain repeatable and reliable results for the preparations examined. The results of the quantitative analysis are characterized by a small confidence interval and are close to the declared contents of active constituents: oxytetracycline 30.01 ± 0.38 g at λ = 350 nm and 30.24 ± 0.86 g at λ = 430 nm; tiamulin, 10.19 ± 0.86 g at λ = 450 nm; lincomycin, 2.27 ± 0.08 g at λ = 278 nm; and spectinomycin, 2.18 ± 0.07 g at λ = 421 nm. The recoveries for all antibiotics ranged from 100.01 to 102.54%.

Use of low volatility mobile phases in electroosmotic thin-layer chromatography

2005 ◽  
Vol 1084 (1-2) ◽  
pp. 13-17 ◽  
Author(s):  
V.G. Berezkin ◽  
A.O. Balushkin ◽  
B.V. Tyaglov ◽  
E.F. Litvin
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phases ◽  
Layer Chromatography
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