Instability of hexane-acetonitrile mobile phases used for the chromatographic analysis of triacylglycerides

2008 ◽  
Vol 31 (6-7) ◽  
pp. NA-NA
Author(s):  
Robert G. Harfmann ◽  
Samir Julka ◽  
Hernan J. Cortes
Keyword(s):  
Chromatographic Analysis ◽  
Mobile Phases

Liquid chromatographic analysis and isolation of diastereoisomers obtained by an aldolization reaction

1995 ◽  
Vol 73 (10) ◽  
pp. 1733-1737 ◽  
Author(s):  
Michel Lafosse ◽  
Philippe Morin ◽  
Christophe Anceau ◽  
Gérard Coudert ◽  
Gérald Guillaumet ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Reaction Mechanism ◽  
Chromatographic Analysis ◽  
Normal Phase ◽  
Structural Determination ◽  
Mobile Phases ◽  
Liquid Chromatographic Analysis ◽  
Phase Liquid ◽  
Liquid Chromatographic

A new route using an aldolization reaction has been investigated in order to synthesize a precursor of pilocarpin, an alkaloid having sympathomimetic properties. To study the stereoselectivity of this route and to propose a mechanism, liquid chromatography has been used to analyse and to isolate each diastereoisomer for a structural determination by NMR. To further investigate the reaction mechanism, seven different aldehydes (or ketones) were used in the aldolization reaction, producing seven different mixtures. This paper describes the improvement of LC conditions with diol- and amino-bonded silica and the nonaqueous mobile phases required for the isolation of each isomer. Keywords: diastereoisomers, pilocarpin precursor, normal phase liquid chromatography, diol- and amino-bonded silicas.

scholarly journals HPLC ASSESSMENT OF VIOLAXANTHIN CYCLE’S FUNCTION UNDER EXCESSIVE LIGHT IN MOUGEOTIA SP. ALGAE

1970 ◽  
Vol 63 ◽  
Author(s):  
E. Muntean ◽  
V. Bercea ◽  
N. Dragoş ◽  
Nicoleta Muntean
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Chromatographic Analysis ◽  
Mobile Phase ◽  
High Performance ◽  
Carotenoid Biosynthesis ◽  
Biological Research ◽  
Protective Function ◽  
Mobile Phases

The protective function of xanthophyll cycle was investigated in the green algae Mougeotia sp. Agardt (AICB 560) originating from the collection belonging to Institute of Biological Research Cluj Napoca, using excessive light. High performance liquid chromatography was used to analyze carotenoids involved in violaxanthin cycle in this matrix. Separations were performed on an Agilent 1100 system, using a Nucleosil 120-5 C18 column and the following mobile phases: A - acetonitrile : water (9 : 1) and B - ethyl acetate. The flow rate of the mobile phase was 1 ml/ min. and the solvent gradient was as follows: from 0 to 20 min. - 10% to 70% B, then from 20 to 30 min. - 70% to 10% B. Chromatographic analysis demonstrated a particular behavior of AICB 560 under intense illumination: the major de-epoxidation product is not zeaxanthin, but antheraxanthin. More than that, the illumination conditions affect the whole carotenoid biosynthesis, starting with the violaxanthin cycle’s pigments precursor: b-carotene.

scholarly journals Fluorescence Detection of Phytoconstituents in Hydroalcoholic Extract of Ipomoea aquatica Forssk. by Thin Layer Chromatographic Analysis

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Sasikala M. ◽  
Sundaraganapathy R.
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Chromatographic Analysis ◽  
High Yield ◽  
Active Metabolites ◽  
Water Spinach ◽  
Hydroalcoholic Extract ◽  
Mobile Phases ◽  
Thin Layer Chromatographic Analysis

Introduction: The medicinal plant water spinach is applicable for many therapeutic activities like anti cancer, anti oxidant, etc. the plant is used for purification of metals and waters of different categories. The benefit of hydroalcoholic extract in this study is non toxic, preservative and high yield of extract from the herbal powder. Thin layer chromatographic analysis is one of the best analytical methods for the separation and identification of phytoconstituents from the crude extract. The spots separated on the stationary phase could be detected by using the non destructive fluorescence method. The detection will be done at shorter and longer wavelength in UV cabinet. Aim and Objective: The aim was to separate and identify the various active metabolites present in the crude extract of the herb of interest using Rf value by thin layer chromatographic analysis. Method: The thin layer chromatographic parameters were set by trial and error method. Ethyl acetate: Methanol: Water (10.0:1.35:1.0 v/v/v) for alkaloids, Ethyl acetate: Pyridine: Water (5.0:1.0:4.0 v/v/v) for glycosides, Toluene: Acetone: Formic acid (4.5:4.5:1.0 v/v/v) for tannins and Toluene: Ethyl acetate (9.3:0.7 v/v) for triterpenoids were the mobile phases for the individual constituents. The constituents were identified at 365nm. Results and Discussion: Nearly 24 various constituents of alkaloids, glycosides, flavonoids, triterpenoids and tannins were separated using respective mobile phases through Rf value. Conclusion: The mentioned TLC profile for the plant of interest will hopefully help the researchers in herbal field to develop more herbal medicines requiring the particular constituent or its extract.

Instability of hexane-acetonitrile mobile phases used for the chromatographic analysis of triacylglycerides

2008 ◽  
Vol 31 (6-7) ◽  
pp. 915-920 ◽  
Author(s):  
Robert G. Harfmann ◽  
Samir Julka ◽  
Hernan J. Cortes
Keyword(s):  
Chromatographic Analysis ◽  
Mobile Phases
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