The influence of nutrient medium and growth regulators on in vitro morphogenesis of Thuja occidentalis L.

2016 ◽  
pp. 153-156
Author(s):  
D Zontikov ◽  
S Zontikova ◽  
R Sergeev ◽  
A Shurgin
Keyword(s):  
Growth Regulators ◽  
Nutrient Medium ◽  
In Vitro Morphogenesis ◽  
Thuja Occidentalis

scholarly journals Introduction to in vitro culture and callus initiation in Salvia hispanica L. (chia)

2019 ◽  
Vol 17 (1) ◽  
pp. 33-37
Author(s):  
A. Z. Revutskaya ◽  
A. V. Holubenko ◽  
N. V. Nuzhyna ◽  
H. O. Rudik ◽  
N. Yu. Taran
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Nutrient Medium ◽  
Callus Growth ◽  
Basic Medium ◽  
High Concentration ◽  
Salvia Hispanica ◽  
Callus Initiation ◽  
Salvia Hispanica L

Aim. Preparation of aseptic seedlings Salvia hispanica L., callus initiation in vitro and establishment of primary explants suitable for the callus production. Methods. Seeds are sprouted on our own modification of conventional methods. The non-hormonal Murashige-Skoog agarized nutrient medium was used as basic medium for the experiments. Parts of one-month seedlings (roots, hypocotyl, cotyledon leaves) were used as explants for the use of the colza. We added growth regulators (BAP, 2,4-D) in different concentration combinations into the nutrient medium for callus initiation. Statistical processing was performed in Microsoft Office Excel. Results. Aseptic S. hispanica seedlings have been obtained. The callus growth was initiated on all types of explants, the dependence of the callus intensity on the type of explants and the growth regulators content in the nutrient medium was established. Morphogenic callus and root-regenerants have been obtained. Conclusions. Hypocotyl was the most suitable primary explant for callus growth. Seedlings, leaves and roots showed low morphogenetic capacity. The nutrient medium with an elevated 2,4-D content was the most effective for initiation of callus genesis and proliferation of non-morphogenous callus. A high concentration of 2,4-D in the medium improves S. hispanica callus growth but suppresses its morphogenic ability.Keywords: Salvia hispanica (Chia), in vitro culture, callus.

Plant genotype and growth regulators interaction affecting in vitro morphogenesis of blackberry and raspberry

1997 ◽  
Vol 39 (1) ◽  
pp. 139-150 ◽  
Author(s):  
B. Mezzetti ◽  
G. Savini ◽  
F. Carnevali ◽  
D. Mott
Keyword(s):  
Growth Regulators ◽  
Plant Genotype ◽  
In Vitro Morphogenesis

scholarly journals Modeling of asymmetric division of somatic cell in protoplasts culture of higher plants

2020 ◽  
Vol 11 (2) ◽  
pp. 255-265
Author(s):  
S. I. Kondratenko ◽  
T. P. Pasternak ◽  
O. P. Samovol ◽  
O. M. Mogilna ◽  
O. V. Sergienko
Keyword(s):  
Cell Division ◽  
Osmotic Stress ◽  
Growth Regulators ◽  
Nutrient Medium ◽  
Higher Plants ◽  
Asymmetric Division ◽  
Cycle Phase ◽  
In Planta ◽  
Selection Of

The key result of the work is the selection of factors for the cultivation of protoplasts of higher plants in vitro, which allowed induction of asymmetrical cell division during the first cell cycle phase. Gibberellin has been proved to be one of the main cofactors of asymmetric division of plant cells. The objects of research were plants of the following cultivars aseptically grown in hormone-free MS medium: tobacco (Nicotiana tabacum L.), SR-1 line; Arabidopsis thaliana var. columbia (L.) Heynh; potato (Solanum tuberosum L.), Zarevo cultivar; cultivated white head cabbage (Brassica oleraceae var. capitata L.) of the following varieties: Kharkivska zymnia, Ukrainska osin, Yaroslavna, Lika, Lesya, Bilosnizhka, Dithmarscher Früher, Iyunskarannya; rape (Brassica napus L.) of Shpat cultivar; winter radish (Raphanus sativus L.) of Odessa-5 cultivar. In experiments with mesophilic and hypocotyl protoplasts of the above-mentioned plant species it has been proved that short-term osmotic stress within 16–18 hours being combined with subsequent introduction of high doses of gibberellin GK3 (1 mg/L) into the modified liquid nutrient media TM and SW led to the occurrence of pronounced morphological traits of cytodifferentiation already at the initial stages of the development of mitotically active cells in a number of higher plants. Meanwhile, in all analyzed species, there was observed the division of the initial genetically homogeneous population of mitotically active cells into two types of asymmetric division: by the type of division of the mother cell into smaller daughter cells and by the type of the first asymmetric division of the zygotic embryo in planta. In this case, the first type of asymmetric division occurred during unusual cytomorphism of the mother cells: a pronounced heart-shaped form even before the first division, which is inherent in the morphology of somatic plant embryo in vitro at the heart-shaped stage. A particular study of the effect of osmotic stress influencing protoplasts of various cultivars of white cabbage, isolated from hypocotyls of 7–9 day etiolated seedlings, revealed quite a typical consistent pattern: the acquisition and maintenance of the axis of symmetry in growing microcolonies occurred without extra exogenous gibberellin (GK3), which was added to the nutrient medium earlier. While analyzing the effect of growth regulators on the formation of microcolonies with traits of structural organization, the conclusion was made regarding the commonality of the revealed morphogenetic reactions of cells within the culture of protoplasts of higher plants in vitro with similar reactions studied earlier on other plants, both in vitro and in planta. Modeling of asymmetric cell division in protoplast culture in vitro has become possible by carrying out a balanced selection of growth regulators as well as their coordinated application through time along with changes in osmotic pressure of a nutrient medium.

scholarly journals A Journey into of the Universe of in vitro Cultures of Plants. Callogenesis

2019 ◽  
Vol 9 (4) ◽  
pp. 45
Author(s):  
Gogu Ghiorghita
Keyword(s):  
Nutrient Medium ◽  
In Vitro Cultures ◽  
Plant Tissues ◽  
Cultivation Conditions ◽  
In Vitro Morphogenesis ◽  
History Of ◽  
The Universe ◽  
Biological Nature

After presenting a brief history of the discovery of this system of cultivation of plant tissues and cells, of the evolution in time of knowledge in this field, of the pathways of in vitro morphogenesis in plants, the paper focuses on in vitro callogenesis in plants. There are presented the types of callus that can be generated via in vitro culture (illustrated with aspects from the author's experience), the influence of factors related to the biological nature of the explants, the composition of the nutrient medium, the cultivation conditions, etc., upon some characteristics of the callus obtained. A special chapter is dedicated to the importance of in vitro cultures of callus in plants.

scholarly journals In vitro morphogenesis of different mangabeira (Hancornia speciosa gomes) varieties from the savanna of the Goiás

2018 ◽  
Vol 11 (5) ◽  
pp. 82-88
Author(s):  
M. C. Vieira ◽  
R. M. Oliviera ◽  
J. B. A. França ◽  
C. C. P. Oliveira ◽  
E. R. B. Souza
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Genetic Improvement ◽  
Fruit Tree ◽  
The Other ◽  
In Vitro Germination ◽  
In Vitro Morphogenesis ◽  
Federal Institute ◽  
Hancornia Speciosa

Hancornia speciosa is a fruit tree that is in the process of domestication. This time the propagation in vitro when well developed can promote conditions for the production of quality seedlings for this fruit. In vitro culture technologies are of great importance for conservation programs of genetic resources and genetic improvement of mangabeira. The objective of this study is to establish a methodology the micropropagation of Hancornia speciosa, Gomes, of three varieties (cuyabensis, gardneri and pubescens) by in vitro germination of seeds under different growth regulators. Fruits were collected at School of Agronomy of the Federal University of Goiás, transported to the Laboratory of Biotechnology, Federal Institute Campus Urutaí - Goiás, inoculated for germination in test tubes containing MS growth medium with 50% of salts concentrations, with five different concentrations of IBA. It was observed that the in vitro germination of cuyabensis, gardneri, and pubescens were 66.00 %, 64.05 % and 76 %, respectively. The beginning of germination occurred from three days for gardneri variety, six days for pubescens variety, and eigh day for cuyabensis variety, extending to the 16 days for gardneri variety, and 28 days for the other varieties. Explants of different varieties differ in their manifestations when cultured in vitro. The aseptic method was effective for the control of fungal agents

scholarly journals REGENERATION CAPACITY OF NARROW-LOCALIZED ENDEMIC SPECIES Dianthus hypanicus Andrz. in vitro

2021 ◽  
Vol 14 (3) ◽  
pp. 39-45
Author(s):  
L.A. Koldar ◽  
Keyword(s):  
Growth Regulators ◽  
Nutrient Medium ◽  
Shoot Formation ◽  
Natural Habitats ◽  
Nutrient Media ◽  
Leafy Shoots ◽  
Mass Reproduction ◽  
Rare And Endangered ◽  
Propagation Methods

Development of effective technology of rare and endangered plant species mass reproduction in vitro is one of the urgent nowadays tasks. Diantus hypanicus Andrz. of the Caryophyllaceae Juss. family belongs to this group of plants. It is an endemic, narrow-localized species listed in the Red Book of Ukraine, the European Red List and the Annex to the Berne Convention. Prospects for the conservation of this species are associated with the study of its viability and require the development of effective reproduction methods. Aim. The purpose of the experiment was to determine the effect of different growth regulators concentrations on the D. hypanicus explants regenerative capacity during in vitro propagation. Methods. For the experiment the seed were used collected from plants of natural habitats and sown on a hormone-free nutrient medium according to the recipe of Murashige and Skoog. For 12–15 days, 4–6 cm long seedlings were obtained which were transferred to nutrient media modified with the content of growth-regulating substances: BAP 0.5–2.0 mg / l, IBA 0.01 mg / l and IAA 0.1 mg / l. Results. Initiation of adventitious buds with subsequent shoot formation was observed for 12–16 days. Each variant plants differed in number, growth activity and intensity, morphometric parameters. After 35–40 days from the explant introduction, conglomerates with well-developed leafy shoots were formed. It was found that, when modifying nutrient media with growth regulators BAP, IBA, IAA of different concentrations, D. hypanicus explants were actively undergoing regeneration processes of different intensity. Conclusions. It was proved that high ability to regenerate was the characteristic of explants cultured on a nutrient medium modified by the addition of BAP – 0.5 mg / l and IAA – 0.1 mg / l, where, on average, 18 ± 0.24 shoots of 2.42 ± 0.17 cm long were formed with a net reproduction of 19.06 ± 0.14. Shoots with 3.0–5.0 pairs of leaves that reached 3.5–6.0 cm were selected and transferred to nutrient media for rooting. Less developed shoots were planted on nutrient media for further reproduction. The next stage of the study is aimed at modifying nutrient media to achieve explants rhizogenesis and obtain a large number of plants necessary for the preservation of this endemic narrow-localized species in culture with subsequent repatriation to natural places of growth.

scholarly journals Influence of growth regulators and nutritional composition on the embryoidogenesis process in Rubus chamaemorus L. in micropores in vitro culture

2020 ◽  
Vol 24 ◽  
pp. 00100
Author(s):  
Dmitry Zontikov ◽  
Igor Krinitsyn ◽  
Svetlana Zontikova ◽  
Ksenia Malakhova ◽  
A. Baghizadeh ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Nutrient Medium ◽  
Glucose Solution ◽  
Nutritional Composition ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Rubus Chamaemorus ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Intensive Growth

The effect of growth regulators and nutrient composition on the embryoidogenesis of Rubus chamaemorus L. (hermaphroditic cultivar Nubu and male plants selected in the Kostroma region) was studied. The density of microspores in the suspension was about 80000 pieces in 1 ml of a sterile aqueous solution with glucose. It was found that for the use of 2,4-dichlorophenoxyacetic acid at a concentration of 0.2 mg/l, it is possible to obtain up to 33 ± 2 pcs. embryoids at a microspore density of 80000 in 0.5 ml of a sterile glucose solution. In an experiment on the influence of the mother liquor of the nutrient medium on the growth of embryoids, it was found that of the available combinations, more intensive growth processes took place on the nutrient medium with a reduced content of macronutrients to 2/3 of the base MS medium.

scholarly journals Effect of growth regulators on direct Miscanthus × giganteus organogenesis in vitro

2018 ◽  
Vol 25 (2) ◽  
Author(s):  
Inga Jančauskienė ◽  
Aušra Blinstrubienė ◽  
Natalija Burbulis ◽  
Ramunė Masienė
Keyword(s):  
Growth Regulators ◽  
Nutrient Medium ◽  
Adventitious Shoots ◽  
Plant Biotechnology ◽  
Research Centre ◽  
Direct Organogenesis ◽  
Joint Research ◽  
Miscanthus Giganteus ◽  
Shoot Number

Research was carried out at the Institute of Biology and Plant Biotechnology of Aleksandras Stulginskis University and at the Laboratory of Agrobiotechnology of the Joint Research Centre. The effect of growth regulators on the direct organogenesis from isolated explants of Miscanthus × giganteus was analysed. Rhizome segments of Miscanthus × giganteus were cultivated in the Murashige and Skoog (MS) nutrient medium supplemented with different 6-benzylaminopurine (BAP) or thidiazuron (TDZ) and ɑ-naftilacetic acid (NAA) combinations. It has been determined that the isolated explants in the nutrient medium without growth regulators did not form adventitious shoots. In the nutrient medium supplemented with growth regulators the regeneration frequency varied from 5.6 to 21.1%. The adventitious shoots formation frequency due to the TDZ exposure, depending on the concentration, was from 5.4 to 10.0% higher (in combination with 0.05 mg l–1 NAA) and from 4.4 to 8.8% higher (in combination with 0.1 mg l–1 NAA) in comparison with the adequate concentration of TDZ. The most intensive shoot formation frequency and the highest shoot number per explant have been obtained in the medium supplemented with 2.0 mg l–1 TDZ + 0.05 mg l–1 NAA.

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