scholarly journals In vitro morphogenesis of different mangabeira (Hancornia speciosa gomes) varieties from the savanna of the Goiás

2018 ◽  
Vol 11 (5) ◽  
pp. 82-88
Author(s):  
M. C. Vieira ◽  
R. M. Oliviera ◽  
J. B. A. França ◽  
C. C. P. Oliveira ◽  
E. R. B. Souza
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Genetic Improvement ◽  
Fruit Tree ◽  
The Other ◽  
In Vitro Germination ◽  
In Vitro Morphogenesis ◽  
Federal Institute ◽  
Hancornia Speciosa

Hancornia speciosa is a fruit tree that is in the process of domestication. This time the propagation in vitro when well developed can promote conditions for the production of quality seedlings for this fruit. In vitro culture technologies are of great importance for conservation programs of genetic resources and genetic improvement of mangabeira. The objective of this study is to establish a methodology the micropropagation of Hancornia speciosa, Gomes, of three varieties (cuyabensis, gardneri and pubescens) by in vitro germination of seeds under different growth regulators. Fruits were collected at School of Agronomy of the Federal University of Goiás, transported to the Laboratory of Biotechnology, Federal Institute Campus Urutaí - Goiás, inoculated for germination in test tubes containing MS growth medium with 50% of salts concentrations, with five different concentrations of IBA. It was observed that the in vitro germination of cuyabensis, gardneri, and pubescens were 66.00 %, 64.05 % and 76 %, respectively. The beginning of germination occurred from three days for gardneri variety, six days for pubescens variety, and eigh day for cuyabensis variety, extending to the 16 days for gardneri variety, and 28 days for the other varieties. Explants of different varieties differ in their manifestations when cultured in vitro. The aseptic method was effective for the control of fungal agents

scholarly journals Introduction to in vitro culture and callus initiation in Salvia hispanica L. (chia)

2019 ◽  
Vol 17 (1) ◽  
pp. 33-37
Author(s):  
A. Z. Revutskaya ◽  
A. V. Holubenko ◽  
N. V. Nuzhyna ◽  
H. O. Rudik ◽  
N. Yu. Taran
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Nutrient Medium ◽  
Callus Growth ◽  
Basic Medium ◽  
High Concentration ◽  
Salvia Hispanica ◽  
Callus Initiation ◽  
Salvia Hispanica L

Aim. Preparation of aseptic seedlings Salvia hispanica L., callus initiation in vitro and establishment of primary explants suitable for the callus production. Methods. Seeds are sprouted on our own modification of conventional methods. The non-hormonal Murashige-Skoog agarized nutrient medium was used as basic medium for the experiments. Parts of one-month seedlings (roots, hypocotyl, cotyledon leaves) were used as explants for the use of the colza. We added growth regulators (BAP, 2,4-D) in different concentration combinations into the nutrient medium for callus initiation. Statistical processing was performed in Microsoft Office Excel. Results. Aseptic S. hispanica seedlings have been obtained. The callus growth was initiated on all types of explants, the dependence of the callus intensity on the type of explants and the growth regulators content in the nutrient medium was established. Morphogenic callus and root-regenerants have been obtained. Conclusions. Hypocotyl was the most suitable primary explant for callus growth. Seedlings, leaves and roots showed low morphogenetic capacity. The nutrient medium with an elevated 2,4-D content was the most effective for initiation of callus genesis and proliferation of non-morphogenous callus. A high concentration of 2,4-D in the medium improves S. hispanica callus growth but suppresses its morphogenic ability.Keywords: Salvia hispanica (Chia), in vitro culture, callus.

scholarly journals Growth regulators and darkness increase efficiency in in vitro culture of immature embryos from peppers

2014 ◽  
Vol 71 (6) ◽  
pp. 488-493 ◽  
Author(s):  
Juan Pablo Manzur ◽  
María de las Nieves Calvache-Asensio ◽  
Adrian Rodriguez-Burruezo
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Immature Embryos ◽  
Increase Efficiency

The Influence of Growth Regulators on In-Vitro Culture of Rosa Hybrida

2019 ◽  
Vol 16 (7) ◽  
pp. 2990-2994
Author(s):  
Kazem Kamali Aliabad ◽  
Amir Rezaee ◽  
Farhad Homayoonfar ◽  
Elahe Zamani
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Rosa Hybrida

scholarly journals The influence of light spectra, UV-A, and growth regulators on the in vitro seed germination of Senecio cineraria DC.

Revista CERES ◽  
2010 ◽  
Vol 57 (5) ◽  
pp. 576-580 ◽  
Author(s):  
Cristiane Pimentel Victório ◽  
Nina Cláudia Barbosa da Silva ◽  
Maria Apparecida Esquibel ◽  
Alice Sato
Keyword(s):  
Seed Germination ◽  
Growth Regulators ◽  
White Light ◽  
Germination Rate ◽  
Germination Percentage ◽  
Ms Medium ◽  
In Vitro Germination ◽  
Light Spectra ◽  
Indole 3 Acetic Acid

This study was carried out to investigate the effects of light spectra, additional UV-A, and different growth regulators on the in vitro germination of Senecio cineraria DC. Seeds were surface-sterilized and inoculated in MS medium to evaluate the following light spectra: white, white plus UV-A, blue, green, red or darkness. The maximum germinability was obtained using MS0 medium under white light (30%) and MS + 0.3 mg L-1 GA3 in the absence of light (30.5%). S. cineraria seeds were indifferent to light. Blue and green lights inhibited germination. Different concentrations of gibberellic acid (GA3) (0.1; 0.4; 0.6; 0.8; 1.0 and 2.0 mg L-1) and indole-3-acetic acid IAA (0.1; 0.3 and 1.0 mg L-1) were evaluated under white light and darkness. No concentration of GA3 enhanced seed germination percentage under white light. However, when the seeds were maintained in darkness, GA3 improved germination responses in all tested concentrations, except at 1.0 mg L-1. Under white light, these concentrations also increased the germination time and reduced germination rate. Germination rate, under light or darkness, was lower using IAA compared with GA3.

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