Efficient asymmetric synthesis of (S)-3-phenyllactic acid by using whole cells of recombinant E.coli

2015 ◽  
pp. 417-422
Keyword(s):  
Asymmetric Synthesis ◽  
Phenyllactic Acid ◽  
Whole Cells ◽  
Recombinant E.Coli

scholarly journals Efficient Conversion of Phenylpyruvic Acid to Phenyllactic Acid by Using Whole Cells of Bacillus coagulans SDM

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e19030 ◽  
Author(s):  
Zhaojuan Zheng ◽  
Cuiqing Ma ◽  
Chao Gao ◽  
Fengsong Li ◽  
Jiayang Qin ◽  
...  
Keyword(s):  
Bacillus Coagulans ◽  
Phenylpyruvic Acid ◽  
Phenyllactic Acid ◽  
Whole Cells ◽  
Efficient Conversion

3‐phenyllactic acid production by free‐whole‐cells of Lactobacillus crustorum in batch and continuous fermentation systems

2020 ◽  
Vol 129 (2) ◽  
pp. 335-344
Author(s):  
J.‐J. Xu ◽  
L.‐J. Fu ◽  
K.‐L. Si ◽  
T.‐L. Yue ◽  
C.‐F. Guo
Keyword(s):  
Acid Production ◽  
Continuous Fermentation ◽  
Phenyllactic Acid ◽  
Whole Cells

scholarly journals Efficient biocatalytic stereoselective reduction of methyl acetoacetate catalyzed by whole cells of engineered E. coli

RSC Advances ◽  
2018 ◽  
Vol 8 (18) ◽  
pp. 9970-9978 ◽  
Author(s):  
Y. H. Cui ◽  
P. Wei ◽  
F. Peng ◽  
M. H. Zong ◽  
W. Y. Lou
Keyword(s):  
Asymmetric Synthesis ◽  
Functional Materials ◽  
Building Blocks ◽  
Methyl Acetoacetate ◽  
Stereoselective Reduction ◽  
Whole Cells ◽  
E Coli ◽  
Hydroxy Esters

Asymmetric synthesis of chiral β-hydroxy esters, the key building blocks for many functional materials, is currently of great interest.

scholarly journals Enantioselective Biosynthesis of l-Phenyllactic Acid by Whole Cells of Recombinant Escherichia coli

Molecules ◽  
2017 ◽  
Vol 22 (11) ◽  
pp. 1966 ◽  
Author(s):  
Yibo Zhu ◽  
Ying Wang ◽  
Jiayuzi Xu ◽  
Jiahao Chen ◽  
Limei Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Phenyllactic Acid ◽  
Whole Cells

Low temperature x-ray microanalysis of frozen-hydrated tobacco leaves

1984 ◽  
Vol 42 ◽  
pp. 284-285
Author(s):  
S. Edith Taylor ◽  
Patrick Echlin ◽  
May McKoon ◽  
Thomas L. Hayes
Keyword(s):  
Low Temperature ◽  
Lemna Minor ◽  
Root Tips ◽  
Tobacco Leaves ◽  
X Ray ◽  
Whole Cells ◽  
Carbon Coated ◽  
The Right ◽  
Beam Currents ◽  
The University

Low temperature x-ray microanalysis (LTXM) of solid biological materials has been documented for Lemna minor L. root tips. This discussion will be limited to a demonstration of LTXM for measuring relative elemental distributions of P,S,Cl and K species within whole cells of tobacco leaves.Mature Wisconsin-38 tobacco was grown in the greenhouse at the University of California, Berkeley and picked daily from the mid-stalk position (leaf #9). The tissue was excised from the right of the mid rib and rapidly frozen in liquid nitrogen slush. It was then placed into an Amray biochamber and maintained at 103K. Fracture faces of the tissue were prepared and carbon-coated in the biochamber. The prepared sample was transferred from the biochamber to the Amray 1000A SEM equipped with a cold stage to maintain low temperatures at 103K. Analyses were performed using a tungsten source with accelerating voltages of 17.5 to 20 KV and beam currents from 1-2nA.

Discovery of intracellular 2,8 dihydroxyadenine crystals in bovine lymphatic and hepatic cells

1987 ◽  
Vol 45 ◽  
pp. 906-907
Author(s):  
W. E. Rigsby ◽  
D. M. Hinton ◽  
V. J. Hurst ◽  
P. C. McCaskey
Keyword(s):  
Polarized Light ◽  
Paraffin Sections ◽  
Tissue Samples ◽  
Whole Cells ◽  
Tissue Sections ◽  
Hepatic Cells ◽  
Slaughter House ◽  
Mammalian Tissues ◽  
Carbon Coated ◽  
Cellular Components

Crystalline intracellular inclusions are rarely seen in mammalian tissues and are often difficult to positively identify. Lymph node and liver tissue samples were obtained from two cows which had been rejected at the slaughter house due to the abnormal appearance of these organs in the animals. The samples were fixed in formaldehyde and some of the fixed material was embedded in paraffin. Examination of the paraffin sections with polarized light microscopy revealed the presence of numerous crystals in both hepatic and lymph tissue sections. Tissue sections were then deparaffinized in xylene, mounted, carbon coated, and examined in a Phillips 505T SEM equipped with a Tracor Northern X-ray Energy Dispersive Spectroscopy (EDS) system. Crystals were obscured by cellular components and membranes so that EDS spectra were only obtainable from whole cells. Tissue samples which had been fixed but not paraffin-embedded were dehydrated, embedded in Spurrs plastic, and sectioned.

High-resolution nucleic acid sequence mapping via in situ hybridization at the Electron Microscope level

1995 ◽  
Vol 53 ◽  
pp. 752-753
Author(s):  
B.A. Hamkalo ◽  
S. Narayanswami ◽  
A.P. Kausch
Keyword(s):  
Nucleic Acid ◽  
Interphase Nucleus ◽  
Genome Mapping ◽  
Precise Location ◽  
Electron Microscope Level ◽  
Rna Sequences ◽  
Fundamental Interest ◽  
Whole Cells ◽  
Dna And Rna

The availability of nonradioactive methods to label nucleic acids an the resultant rapid and greater sensitivity of detection has catapulted the technique of in situ hybridization to become the method of choice to locate of specific DNA and RNA sequences on chromosomes and in whole cells in cytological preparations in many areas of biology. It is being applied to problems of fundamental interest to basic cell and molecular biologists such as the organization of the interphase nucleus in the context of putative functional domains; it is making major contributions to genome mapping efforts; and it is being applied to the analysis of clinical specimens. Although fluorescence detection of nucleic acid hybrids is routinely used, certain questions require greater resolution. For example, very closely linked sequences may not be separable using fluorescence; the precise location of sequences with respect to chromosome structures may be below the resolution of light microscopy(LM); and the relative positions of sequences on very small chromosomes may not be feasible.

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