Cell Biology Research on Stem Cells, Aging, Cancer Resistance, and Epigenetics in Marbled Crayfish and Relatives: Potential Benefi ts for Human Biology and Medicine

2015 ◽  
pp. 115-158 ◽  
Author(s):  
Günter Vogt
Keyword(s):  
Stem Cells ◽  
Cell Biology ◽  
Human Biology ◽  
Cancer Resistance ◽  
Marbled Crayfish ◽  
Biology Research

scholarly journals Stem cells: the new “model organism”

2017 ◽  
Vol 28 (11) ◽  
pp. 1409-1411 ◽  
Author(s):  
David G. Drubin ◽  
Anthony A. Hyman
Keyword(s):  
Stem Cells ◽  
Tissue Culture ◽  
Stem Cell ◽  
Human Tissue ◽  
Cell Biology ◽  
Physiological Function ◽  
Model Organism ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Biology Research

Human tissue culture cells have long been a staple of molecular and cell biology research. However, although these cells are derived from humans, they have often lost considerable aspects of natural physiological function. Here we argue that combined advances in genome editing, stem cell production, and organoid derivation from stem cells represent a revolution in cell biology. These advances have important ramifications for the study of basic cell biology mechanisms, as well as for the ways in which discoveries in mechanisms are translated into understanding of disease.

Induced Pluripotent Stem Cells on a Chip: A Self-Contained, Accessible, Pipette-less iPSC Culturing and Differentiation Kit

2020 ◽  
pp. 247263032092117
Author(s):  
Patrick McMinn ◽  
David J. Guckenberger ◽  
David J. Beebe
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cell Biology ◽  
Full Range ◽  
Stem Cell Maintenance ◽  
Specialized Equipment ◽  
Induced Pluripotent ◽  
Biology Research

Over the past decade, induced pluripotent stem cells (iPSCs) have become a major focus of stem cell and developmental biology research, offering researchers a clinically relevant source of cells that are amenable to genetic engineering approaches. Though stem cells are promising for both research and commercial endeavors, iPSC-based assays require tedious protocols that include complex treatments, expensive reagents, and specialized equipment that limit their integration into academic curricula and cell biology research groups. Expanding on existing Kit-On-A-Lid-Assay (KOALA) technologies, we have developed a self-contained, injection molded, pipette-less iPSC culture and differentiation platform that significantly reduces associated costs and labor of stem cell maintenance and differentiation. The KOALA kit offers users the full range of iPSC culture necessities, including cell cryopreservation, media exchanges, differentiation, endpoint analysis, and a new capability, cell passaging. Using the KOALA kit, we were able to culture ~20,000 iPSCs per microchannel for at least 7 days, while maintaining stable expression of stemness markers (SSEA4 and Oct4) and normal iPSC phenotype. We also adapted protocols for differentiating iPSCs into neuroepithelial cells, cardiomyocytes, and definitive endodermal cells, a cell type from each germ layer of human development.

scholarly journals Stem Cells: A Renaissance in Human Biology Research

Cell ◽  
2016 ◽  
Vol 165 (7) ◽  
pp. 1572-1585 ◽  
Author(s):  
Jun Wu ◽  
Juan Carlos Izpisua Belmonte
Keyword(s):  
Stem Cells ◽  
Human Biology ◽  
Biology Research

scholarly journals Brain Cancer Stem Cells in Adults and Children: Cell Biology and Therapeutic Implications

2017 ◽  
Vol 14 (2) ◽  
pp. 372-384 ◽  
Author(s):  
Tamara J. Abou-Antoun ◽  
James S. Hale ◽  
Justin D. Lathia ◽  
Stephen M. Dombrowski
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Brain Cancer ◽  
Cell Biology ◽  
Therapeutic Implications ◽  
Adults And Children

scholarly journals Potential Use of Stem Cells for Kidney Regeneration

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Takashi Yokoo ◽  
Kei Matsumoto ◽  
Shinya Yokote
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Research ◽  
Cell Biology ◽  
Current Knowledge ◽  
Kidney Regeneration ◽  
Major Step ◽  
Kidney Dialysis ◽  
Organ Regeneration ◽  
Renal Stem Cell

Significant advances have been made in stem cell research over the past decade. A number of nonhematopoietic sources of stem cells (or progenitor cells) have been identified, including endothelial stem cells and neural stem cells. These discoveries have been a major step toward the use of stem cells for potential clinical applications of organ regeneration. Accordingly, kidney regeneration is currently gaining considerable attention to replace kidney dialysis as the ultimate therapeutic strategy for renal failure. However, due to anatomic complications, the kidney is believed to be the hardest organ to regenerate; it is virtually impossible to imagine such a complicated organ being completely rebuilt from pluripotent stem cells by gene or chemical manipulation. Nevertheless, several groups are taking on this big challenge. In this manuscript, current advances in renal stem cell research are reviewed and their usefulness for kidney regeneration discussed. We also reviewed the current knowledge of the emerging field of renal stem cell biology.

scholarly journals Lineage specification of early embryos and embryonic stem cells at the dawn of enabling technologies

2017 ◽  
Vol 4 (4) ◽  
pp. 533-542 ◽  
Author(s):  
Guangdun Peng ◽  
Patrick P. L. Tam ◽  
Naihe Jing
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Biology ◽  
Developmental Process ◽  
Embryonic Stem ◽  
Molecular Signaling ◽  
Lineage Specification ◽  
Genomic Technologies ◽  
Enabling Technologies ◽  
Stem Cell Pluripotency

Abstract Establishment of progenitor cell populations and lineage diversity during embryogenesis and the differentiation of pluripotent stem cells is a fascinating and intricate biological process. Conceptually, an understanding of this developmental process provides a framework to integrate stem-cell pluripotency, cell competence and differentiating potential with the activity of extrinsic and intrinsic molecular determinants. The recent advent of enabling technologies of high-resolution transcriptome analysis at the cellular, population and spatial levels proffers the capability of gaining deeper insights into the attributes of the gene regulatory network and molecular signaling in lineage specification and differentiation. In this review, we provide a snapshot of the emerging enabling genomic technologies that contribute to the study of development and stem-cell biology.

scholarly journals First person – Federico Pecori

2020 ◽  
Vol 133 (20) ◽  
pp. jcs255166
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Cell Biology ◽  
Embryonic Stem ◽  
Early Career ◽  
First Person ◽  
Receptor Endocytosis ◽  
Early Career Researchers ◽  
Selection Of

ABSTRACTFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Federico Pecori is first author on ‘Mucin-type O-glycosylation controls pluripotency in mouse embryonic stem cells via Wnt receptor endocytosis’, published in JCS. Federico is a PhD student in the lab of Shoko Nishihara at the Laboratory of Cell Biology, Department of Bioinformatics, Soka University, Tokyo, Japan, where he is interested in the mechanisms regulating stem cell identity.

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