Abundance and Diversity of Bacterial Nitrifi ers and Denitrifi ers and Their Functional Genes in Tannery Wastewater Treatment Plants Revealed by High-Throughput Sequencing

2016 ◽  
pp. 128-151
Keyword(s):  
Wastewater Treatment ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Wastewater Treatment Plants ◽  
Tannery Wastewater ◽  
Functional Genes ◽  
Abundance And Diversity

scholarly journals Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 699
Author(s):  
Hui Han ◽  
Mohan Bai ◽  
Yanting Chen ◽  
Yali Gong ◽  
Ming Wu ◽  
...  
Keyword(s):  
High Throughput ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Resistant Bacteria ◽  
Arable Soils ◽  
Soil Bacterial Communities ◽  
Sulfonamide Resistance ◽  
Loam Soil ◽  
Abundance And Diversity ◽  
Sulfonamide Resistance Genes

Although composting is effective in deactivating antibiotic substances in manure, the influence of compost fertilization on the occurrence and dissemination of antibiotic resistance in arable soils remains to be controversial. Herein, the abundance and diversity of two sulfonamide resistance genes (sul1 and sul2) in soil fertilized by compost spiked with two concentrations of sulfadiazine (1 and 10 mg kg−1) were studied intensively by qPCR and high throughput sequencing based on a two-month microcosm experiment. The concentration of sulfadiazine decreased rapidly after spiking from 25% at Day 1 to less than 2.7% at Day 60. Relative abundance of both sul1 and sul2 were significantly higher in soil amended with compost than the non-amended control at Day 1 and slightly decreased with incubation time except for sul2 in the S10 treatment. Soil bacterial communities were transiently shifted by compost fertilization regardless of the presence of sulfadiazine. Relative abundance of genera in three hubs positively interlinked with sul1 and sul2 were significantly higher in compost treated soil than the control at Day 1, 7 and 21, but not at Day 60. High throughput sequencing analyses revealed that most detected (>67% in relative abundance) sul1 and sul2 genotypes sharing >99% similarity with those found in gammaproteobacterial pathogens frequently were commonly present in compost and soil. These results indicated that compost fertilization might increase the abundance rather than diversity of sulfadiazine-resistant populations in soil, which may be facilitated by the presence of sulfadiazine.

scholarly journals Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

2015 ◽  
Vol 81 (20) ◽  
pp. 7215-7222 ◽  
Author(s):  
B. Prevost ◽  
F. S. Lucas ◽  
K. Ambert-Balay ◽  
P. Pothier ◽  
L. Moulin ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Clinical Data ◽  
High Throughput ◽  
Human Population ◽  
High Throughput Sequencing ◽  
Treatment Plant ◽  
Enteric Viruses ◽  
Viral Diversity ◽  
Wwtp Effluent ◽  
Human Astroviruses

ABSTRACTAlthough clinical epidemiology lists human enteric viruses to be among the primary causes of acute gastroenteritis in the human population, their circulation in the environment remains poorly investigated. These viruses are excreted by the human population into sewers and may be released into rivers through the effluents of wastewater treatment plants (WWTPs). In order to evaluate the viral diversity and loads in WWTP effluents of the Paris, France, urban area, which includes about 9 million inhabitants (approximately 15% of the French population), the seasonal occurrence of astroviruses and noroviruses in 100 WWTP effluent samples was investigated over 1 year. The coupling of these measurements with a high-throughput sequencing approach allowed the specific estimation of the diversity of human astroviruses (human astrovirus genotype 1 [HAstV-1], HAstV-2, HAstV-5, and HAstV-6), 7 genotypes of noroviruses (NoVs) of genogroup I (NoV GI.1 to NoV GI.6 and NoV GI.8), and 16 genotypes of NoVs of genogroup II (NoV GII.1 to NoV GII.7, NoV GII.9, NoV GII.12 to NoV GII.17, NoV GII.20, and NoV GII.21) in effluent samples. Comparison of the viral diversity in WWTP effluents to the viral diversity found by analysis of clinical data obtained throughout France underlined the consistency between the identified genotypes. However, some genotypes were locally present in effluents and were not found in the analysis of the clinical data. These findings could highlight an underestimation of the diversity of enteric viruses circulating in the human population. Consequently, analysis of WWTP effluents could allow the exploration of viral diversity not only in environmental waters but also in a human population linked to a sewerage network in order to better comprehend viral epidemiology and to forecast seasonal outbreaks.

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