Detecting Human Bacterial Pathogens in Wastewater Treatment Plants by a High-Throughput Shotgun Sequencing Technique

2013 ◽  
Vol 47 (10) ◽  
pp. 5433-5441 ◽  
Author(s):  
Lin Cai ◽  
Tong Zhang
2021 ◽  
Vol 408 ◽  
pp. 124855
Author(s):  
Houpu Zhang ◽  
Zihan Zhang ◽  
Jiajin Song ◽  
Lin Cai ◽  
Yunlong Yu ◽  
...  

Author(s):  
Kamyar Mehrabi ◽  
Ralf Kaegi ◽  
Detlef Günther ◽  
Alexander Gundlach-Graham

High-throughput single-particle ICP-TOFMS analyses are used to quantify and classify diverse metal nanoparticle types from wastewater treatment plant samples.


2019 ◽  
Vol 7 (11) ◽  
pp. 506 ◽  
Author(s):  
Cecilia Oluseyi Osunmakinde ◽  
Ramganesh Selvarajan ◽  
Bhekie B. Mamba ◽  
Titus A.M. Msagati

Next-generation sequencing provides new insights into the diversity and structure of bacterial communities, as well as the fate of pathogens in wastewater treatment systems. In this study, the bacterial community structure and the presence of pathogenic bacteria in three wastewater treatment plants across Gauteng province in South Africa were studied. The physicochemical results indicated that the quality of wastewater varies considerably from one plant to the others. Proteobacteria, Actinobacteria, Firmicutes, and Chloroflexi were the dominant phyla across the three wastewater treatment plants, while Alphaproteobacteria, Actinobacteria, Bacilli, and Clostridia were the dominant classes. The dominant bacterial functions were highly associated with carbohydrate, energy, and amino acid metabolism. In addition, potential pathogenic bacterial members identified from the influent/effluent samples included Roseomonas, Bacillus, Pseudomonas, Clostridium, Mycobacterium, Methylobacterium, and Aeromonas. The results of linear discriminant analysis (LDA) effect size analysis also confirmed that these bacterial pathogens were significantly abundant in the wastewater treatment systems. Further, the results of this study highlighted that the presence of bacterial pathogens in treated effluent pose a potential contamination risk, transmitted through soil, agriculture, water, or sediments. There is thus a need for continuous monitoring of potential pathogens in wastewater treatment plants (WWTPs) in order to minimize public health risk.


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