Microbial Structures, Functions, and Metabolic Pathways in Wastewater Treatment Bioreactors Revealed Using High-Throughput Sequencing

2012 ◽  
Vol 46 (24) ◽  
pp. 13244-13252 ◽  
Author(s):  
Lin Ye ◽  
Tong Zhang ◽  
Taitao Wang ◽  
Zhiwei Fang
Keyword(s):  
Wastewater Treatment ◽  
High Throughput ◽  
Metabolic Pathways ◽  
High Throughput Sequencing ◽  
Microbial Structures

scholarly journals Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

2015 ◽  
Vol 81 (20) ◽  
pp. 7215-7222 ◽  
Author(s):  
B. Prevost ◽  
F. S. Lucas ◽  
K. Ambert-Balay ◽  
P. Pothier ◽  
L. Moulin ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Clinical Data ◽  
High Throughput ◽  
Human Population ◽  
High Throughput Sequencing ◽  
Treatment Plant ◽  
Enteric Viruses ◽  
Viral Diversity ◽  
Wwtp Effluent ◽  
Human Astroviruses

ABSTRACTAlthough clinical epidemiology lists human enteric viruses to be among the primary causes of acute gastroenteritis in the human population, their circulation in the environment remains poorly investigated. These viruses are excreted by the human population into sewers and may be released into rivers through the effluents of wastewater treatment plants (WWTPs). In order to evaluate the viral diversity and loads in WWTP effluents of the Paris, France, urban area, which includes about 9 million inhabitants (approximately 15% of the French population), the seasonal occurrence of astroviruses and noroviruses in 100 WWTP effluent samples was investigated over 1 year. The coupling of these measurements with a high-throughput sequencing approach allowed the specific estimation of the diversity of human astroviruses (human astrovirus genotype 1 [HAstV-1], HAstV-2, HAstV-5, and HAstV-6), 7 genotypes of noroviruses (NoVs) of genogroup I (NoV GI.1 to NoV GI.6 and NoV GI.8), and 16 genotypes of NoVs of genogroup II (NoV GII.1 to NoV GII.7, NoV GII.9, NoV GII.12 to NoV GII.17, NoV GII.20, and NoV GII.21) in effluent samples. Comparison of the viral diversity in WWTP effluents to the viral diversity found by analysis of clinical data obtained throughout France underlined the consistency between the identified genotypes. However, some genotypes were locally present in effluents and were not found in the analysis of the clinical data. These findings could highlight an underestimation of the diversity of enteric viruses circulating in the human population. Consequently, analysis of WWTP effluents could allow the exploration of viral diversity not only in environmental waters but also in a human population linked to a sewerage network in order to better comprehend viral epidemiology and to forecast seasonal outbreaks.

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