Omics Applications in Brassica Species

2013 ◽  
pp. 163-190 ◽  
Author(s):  
Xiaonan Li ◽  
PhD Ramchiary ◽  
PhD Dhandapani ◽  
PhD Choi ◽  
Yong Lim
Keyword(s):  
Brassica Species

scholarly journals Flavonoid Glycosides in Brassica Species Respond to UV-B Depending on Exposure Time and Adaptation Time

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 494
Author(s):  
Susanne Neugart ◽  
Christiane Bumke-Vogt
Keyword(s):  
Brassica Species ◽  
Flavonoid Glycoside ◽  
Ultraviolet B ◽  
Flavonoid Glycosides ◽  
Production Cycle ◽  
Short Term ◽  
Greenhouse Production ◽  
Adaptation Time ◽  
Quercetin Glycosides ◽  
Kaempferol Glycosides

Recently, there have been efforts to use ultraviolet-B radiation (UV-B) as a biotechnological tool in greenhouses. Leafy Brassica species are mainly considered for their ability to synthesize glucosinolates and are valued as baby salads. They also have a remarkable concentration of chemically diverse flavonoid glycosides. In this study, the effect of short-term UV-B radiation at the end of the production cycle was investigated without affecting plant growth. The aim was to verify which exposure and adaptation time was suitable and needs to be further investigated to use UV as a biotechnological tool in greenhouse production of Brassica species. It is possible to modify the flavonoid glycoside profile of leafy Brassica species by increasing compounds that appear to have potentially high antioxidant activity. Exemplarily, the present experiment shows that kaempferol glycosides may be preferred over quercetin glycosides in response to UV-B in Brassica rapa ssp. chinensis, for example, whereas other species appear to prefer quercetin glycosides over kaempferol glycosides, such as Brassica oleracea var. sabellica or Brassica carinata. However, the response to short-term UV-B treatment is species-specific and conclusions on exposure and adaptation time cannot be unified but must be drawn separately for each species.

scholarly journals Recombination and Selection at Brassica Self-Incompatibility Loci

Genetics ◽  
1999 ◽  
Vol 152 (1) ◽  
pp. 413-425 ◽  
Author(s):  
Philip Awadalla ◽  
Deborah Charlesworth
Keyword(s):  
Linkage Disequilibrium ◽  
Balancing Selection ◽  
Brassica Species ◽  
Selective Constraint ◽  
Intragenic Recombination ◽  
Self Incompatibility ◽  
History Of ◽  
Unknown Gene ◽  
Slg Gene ◽  
High Diversity

Abstract In Brassica species, self-incompatibility is controlled genetically by haplotypes involving two known genes, SLG and SRK, and possibly an as yet unknown gene controlling pollen incompatibility types. Alleles at the incompatibility loci are maintained by frequency-dependent selection, and diversity at SLG and SRK appears to be very ancient, with high diversity at silent and replacement sites, particularly in certain “hypervariable portions of the genes. It is important to test whether recombination occurs in these genes before inferences about function of different parts of the genes can be made from patterns of diversity within their sequences. In addition, it has been suggested that, to maintain the relationship between alleles within a given S-haplotype, recombination is suppressed in the S-locus region. The high diversity makes many population genetic measures of recombination inapplicable. We have analyzed linkage disequilibrium within the SLG gene of two Brassica species, using published coding sequences. The results suggest that intragenic recombination has occurred in the evolutionary history of these alleles. This is supported by patterns of synonymous nucleotide diversity within both the SLG and SRK genes, and between domains of the SRK gene. Finally, clusters of linkage disequilibrium within the SLG gene suggest that hypervariable regions are under balancing selection, and are not merely regions of relaxed selective constraint.

Rapid-cycling Brassica Species: Inbreeding and Selection of B. campestris for Anther Culture Ability

1990 ◽  
Vol 65 (5) ◽  
pp. 557-566 ◽  
Author(s):  
F. N. ASLAM ◽  
M. V. MACDONALD ◽  
P. LOUDON ◽  
D. S. INGRAM
Keyword(s):  
Anther Culture ◽  
Brassica Species ◽  
Rapid Cycling ◽  
Selection Of

Development of a Brassica seed cDNA microarray

Genome ◽  
2008 ◽  
Vol 51 (3) ◽  
pp. 236-242 ◽  
Author(s):  
Daoquan Xiang ◽  
Raju Datla ◽  
Fengling Li ◽  
Adrian Cutler ◽  
Meghna R. Malik ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Cdna Array ◽  
Brassica Species ◽  
Crop Species ◽  
Rna Targets ◽  
Associated Functions ◽  
Leaf Tissues ◽  
Germinating Seeds ◽  
Signal Intensities

Brassica species represent several important crops including canola ( Brassica napus ). Understanding of genetic elements that contribute to seed-associated functions will impact future improvements in the canola crop. Brassica species share a very close taxonomic and molecular relationship with Arabidopsis thaliana. However, there are several subtle but distinct seed-associated agronomic characteristics that differ among the oil seed crop species. To address these, we have generated 67 535 ESTs predominately from Brassica seeds, analyzed these sequences, and identified 10 642 unigenes for the preparation of a targeted seed cDNA array. A set of 10 642 PCR primer pairs was designed and corresponding amplicons were produced for spotting, along with relevant controls. Critical quality control tests produced satisfactory results for use of this microarray in biological experiments. The microarray was also tested with specific RNA targets from embryos, germinating seeds, and leaf tissues. The hybridizations, signal intensities, and overall quality of these slides were consistent and reproducible. Additionally, there are 429 ESTs represented on the array that show no homology with any A. thaliana annotated gene or any gene in the Brassica genome databases or other plant databases; however, all of these probes hybridized to B. napus transcripts, indicating that the array also will be useful in defining expression patterns for genes so far unique to Brassica species.

Biochemical characterisation of an aldoxime-forming flavoprotein involved in 2-phenylethylglucosinolate biosynthesis in Brassica species

1999 ◽  
Vol 37 (2) ◽  
pp. 99-108 ◽  
Author(s):  
Mark F. Oldfield ◽  
Richard N. Bennett ◽  
Guy Kiddle ◽  
Roger M. Wallsgrove ◽  
Nigel P. Botting
Keyword(s):  
Brassica Species ◽  
Biochemical Characterisation

Lutein and β-carotene content of green leafy Brassica species grown under different conditions

LWT ◽  
2013 ◽  
Vol 53 (1) ◽  
pp. 378-381 ◽  
Author(s):  
Constance Reif ◽  
Eva Arrigoni ◽  
Florence Berger ◽  
Daniel Baumgartner ◽  
Laura Nyström
Keyword(s):  
Brassica Species ◽  
Carotene Content ◽  
Β Carotene
Sign in / Sign up

Export Citation Format

Share Document