- Marine Plants and Algae as Promising 3D Scaffolds for Tissue Engineering

2013 ◽  
pp. 564-583
Keyword(s):  
Tissue Engineering ◽  
3D Scaffolds ◽  
Marine Plants

3D Printing & Pharmaceutical Manufacturing: Opportunities and Challenges

2016 ◽  
Vol 5 (01) ◽  
pp. 4723 ◽  
Author(s):  
Bhusnure O. G.* ◽  
Gholve V. S. ◽  
Sugave B. K. ◽  
Dongre R. C. ◽  
Gore S. A. ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
3D Printing ◽  
Computer Aided Design ◽  
Patient Specific ◽  
Computer Design ◽  
3D Scaffolds ◽  
Engineering Research ◽  
Advantages And Disadvantages ◽  
Computer Aided

Many researchers have attempted to use computer-aided design (C.A.D) and computer-aided manufacturing (CAM) to realize a scaffold that provides a three-dimensional (3D) environment for regeneration of tissues and organs. As a result, several 3D printing technologies, including stereolithography, deposition modeling, inkjet-based printing and selective laser sintering have been developed. Because these 3D printing technologies use computers for design and fabrication, and they can fabricate 3D scaffolds as designed; as a consequence, they can be standardized. Growth of target tissues and organs requires the presence of appropriate growth factors, so fabrication of 3Dscaffold systems that release these biomolecules has been explored. A drug delivery system (D.D.S) that administrates a pharmaceutical compound to achieve a therapeutic effect in cells, animals and humans is a key technology that delivers biomolecules without side effects caused by excessive doses. 3D printing technologies and D. D. Ss have been assembled successfully, so new possibilities for improved tissue regeneration have been suggested. If the interaction between cells and scaffold system with biomolecules can be understood and controlled, and if an optimal 3D tissue regenerating environment is realized, 3D printing technologies will become an important aspect of tissue engineering research in the near future. 3D Printing promises to produce complex biomedical devices according to computer design using patient-specific anatomical data. Since its initial use as pre-surgical visualization models and tooling molds, 3D Printing has slowly evolved to create one-of-a-kind devices, implants, scaffolds for tissue engineering, diagnostic platforms, and drug delivery systems. Fuelled by the recent explosion in public interest and access to affordable printers, there is renewed interest to combine stem cells with custom 3D scaffolds for personalized regenerative medicine. Before 3D Printing can be used routinely for the regeneration of complex tissues (e.g. bone, cartilage, muscles, vessels, nerves in the craniomaxillofacial complex), and complex organs with intricate 3D microarchitecture (e.g. liver, lymphoid organs), several technological limitations must be addressed. Until recently, tablet designs had been restricted to the relatively small number of shapes that are easily achievable using traditional manufacturing methods. As 3D printing capabilities develop further, safety and regulatory concerns are addressed and the cost of the technology falls, contract manufacturers and pharmaceutical companies that experiment with these 3D printing innovations are likely to gain a competitive edge. This review compose the basics, types & techniques used, advantages and disadvantages of 3D printing

scholarly journals Binary Biocompatible CNC–Gelatine Hydrogel as 3D Scaffolds Suitable for Cell Culture Adhesion and Growth

Applied Nano ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 118-127
Author(s):  
Luca Zoia ◽  
Anna Binda ◽  
Laura Cipolla ◽  
Ilaria Rivolta ◽  
Barbara La Ferla
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Cell Cultures ◽  
Cellulose Nanocrystals ◽  
Potential Application ◽  
Chemical Properties ◽  
Cellular Adhesion ◽  
Chemical Crosslinking ◽  
3D Scaffolds ◽  
Physical Chemical

Binary nano-biocomposite 3D scaffolds of cellulose nanocrystals (CNCs)—gelatine were fabricated without using chemical crosslinking additives. Controlled oxidative treatment allowed introducing carboxyl or carbonyl functionalities on the surface of CNCs responsible for the crosslinking of gelatine polymers. The obtained composites were characterized for their physical-chemical properties. Their biocompatibility towards different cell cultures was evaluated through MTT and LDH assays, cellular adhesion and proliferation experiments. Gelatine composites reinforced with carbonyl-modified CNCs showed the most performing swelling/degradation profile and the most promising adhesion and proliferation properties towards cell lines, suggesting their potential application in the field of tissue engineering.

Co-delivery of Simvastatin and Demineralized Bone Matrix Hierarchically from Nanosheet-based Supramolecular Hydrogels for Osteogenesis

2021 ◽  
Author(s):  
Xiao Zhang ◽  
Jiabing Fan ◽  
Chen Chen ◽  
Tara Aghaloo ◽  
Min Lee
Keyword(s):  
Tissue Engineering ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Therapeutic Agents ◽  
Engineering Applications ◽  
3D Scaffolds ◽  
Hydrogel Scaffolds ◽  
Delivery Platform ◽  
Weak Compatibility ◽  
Demineralized Bone

Supramolecular hydrogels are widely used as 3D scaffolds and delivery platform in tissue engineering applications. However, hydrophobic therapeutic agents exhibit weak compatibility in hydrogel scaffolds along with aggregation and precipitate....

Development of novel 3D scaffolds using BioExtruder by varying the content of hydroxyapatite and silica in PCL matrix for bone tissue engineering

2020 ◽  
Vol 27 (4) ◽  
Author(s):  
Nandini A. Pattanashetti ◽  
Tania Viana ◽  
Nuno Alves ◽  
Geoffrey R. Mitchell ◽  
Mahadevappa Y. Kariduraganavar
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
3D Scaffolds

scholarly journals Suture Fiber Reinforcement of a 3D Printed Gelatin Scaffold for Its Potential Application in Soft Tissue Engineering

2021 ◽  
Vol 22 (21) ◽  
pp. 11600
Author(s):  
Dong Jin Choi ◽  
Kyoung Choi ◽  
Sang Jun Park ◽  
Young-Jin Kim ◽  
Seok Chung ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Strength ◽  
Physical Properties ◽  
Dimensional Stability ◽  
3D Structure ◽  
Biological Properties ◽  
Dermal Fibroblasts ◽  
3D Scaffolds ◽  
3D Printed

Gelatin has excellent biological properties, but its poor physical properties are a major obstacle to its use as a biomaterial ink. These disadvantages not only worsen the printability of gelatin biomaterial ink, but also reduce the dimensional stability of its 3D scaffolds and limit its application in the tissue engineering field. Herein, biodegradable suture fibers were added into a gelatin biomaterial ink to improve the printability, mechanical strength, and dimensional stability of the 3D printed scaffolds. The suture fiber reinforced gelatin 3D scaffolds were fabricated using the thermo-responsive properties of gelatin under optimized 3D printing conditions (−10 °C cryogenic plate, 40–80 kPa pneumatic pressure, and 9 mm/s printing speed), and were crosslinked using EDC/NHS to maintain their 3D structures. Scanning electron microscopy images revealed that the morphologies of the 3D printed scaffolds maintained their 3D structure after crosslinking. The addition of 0.5% (w/v) of suture fibers increased the printing accuracy of the 3D printed scaffolds to 97%. The suture fibers also increased the mechanical strength of the 3D printed scaffolds by up to 6-fold, and the degradation rate could be controlled by the suture fiber content. In in vitro cell studies, DNA assay results showed that human dermal fibroblasts’ proliferation rate of a 3D printed scaffold containing 0.5% suture fiber was 10% higher than that of a 3D printed scaffold without suture fibers after 14 days of culture. Interestingly, the supplement of suture fibers into gelatin biomaterial ink was able to minimize the cell-mediated contraction of the cell cultured 3D scaffolds over the cell culture period. These results show that advanced biomaterial inks can be developed by supplementing biodegradable fibers to improve the poor physical properties of natural polymer-based biomaterial inks.

3D scaffolds for brain tissue regeneration: architectural challenges

2018 ◽  
Vol 6 (11) ◽  
pp. 2812-2837 ◽  
Author(s):  
Gillian Dumsile Mahumane ◽  
Pradeep Kumar ◽  
Lisa Claire du Toit ◽  
Yahya Essop Choonara ◽  
Viness Pillay
Keyword(s):  
Tissue Engineering ◽  
Brain Tissue ◽  
Tissue Regeneration ◽  
Critical Analysis ◽  
Experimental Studies ◽  
3D Scaffolds

Critical analysis of experimental studies on 3D scaffolds for brain tissue engineering.

scholarly journals Multi-photon polymerization of bio-inspired, thymol-functionalized hybrid materials with biocompatible and antimicrobial activity

2020 ◽  
Vol 11 (25) ◽  
pp. 4078-4083
Author(s):  
Kostas Parkatzidis ◽  
Maria Chatzinikolaidou ◽  
Eleftherios Koufakis ◽  
Maria Kaliva ◽  
Maria Farsari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Antimicrobial Activity ◽  
Hybrid Materials ◽  
Antimicrobial Action ◽  
Dental Tissue ◽  
3D Scaffolds ◽  
Dental Tissue Engineering

Thymyl-methacrylate functionalized, hybrid 3D scaffolds, fabricated by multi-photon lithography, exhibit excellent biocompatibility and antimicrobial action for bone and dental tissue engineering.

scholarly journals Graphene-based 3D scaffolds in tissue engineering: fabrication, applications, and future scope in liver tissue engineering

2019 ◽  
Vol Volume 14 ◽  
pp. 5753-5783 ◽  
Author(s):  
Renu Geetha Bai ◽  
Kasturi Muthoosamy ◽  
Sivakumar Manickam ◽  
Ali Hilal-Alnaqbi
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
3D Scaffolds ◽  
Liver Tissue Engineering

scholarly journals Naturally Prefabricated Marine Biomaterials: Isolation and Applications of Flat Chitinous 3D Scaffolds from Ianthella labyrinthus (Demospongiae: Verongiida)

2019 ◽  
Vol 20 (20) ◽  
pp. 5105 ◽  
Author(s):  
Mario Schubert ◽  
Björn Binnewerg ◽  
Alona Voronkina ◽  
Lyubov Muzychka ◽  
Marcin Wysokowski ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Marine Sponges ◽  
Biologically Active ◽  
3D Scaffolds ◽  
Chemical Treatments ◽  
The Family ◽  
Unique Source ◽  
Induced Pluripotent ◽  
First Time

Marine sponges remain representative of a unique source of renewable biological materials. The demosponges of the family Ianthellidae possess chitin-based skeletons with high biomimetic potential. These three-dimensional (3D) constructs can potentially be used in tissue engineering and regenerative medicine. In this study, we focus our attention, for the first time, on the marine sponge Ianthella labyrinthus Bergquist & Kelly-Borges, 1995 (Demospongiae: Verongida: Ianthellidae) as a novel potential source of naturally prestructured bandage-like 3D scaffolds which can be isolated simultaneously with biologically active bromotyrosines. Specifically, translucent and elastic flat chitinous scaffolds have been obtained after bromotyrosine extraction and chemical treatments of the sponge skeleton with alternate alkaline and acidic solutions. For the first time, cardiomyocytes differentiated from human induced pluripotent stem cells (iPSC-CMs) have been used to test the suitability of I. labyrinthus chitinous skeleton as ready-to-use scaffold for their cell culture. Results reveal a comparable attachment and growth on isolated chitin-skeleton, compared to scaffolds coated with extracellular matrix mimetic Geltrex®. Thus, the natural, unmodified I. labyrinthus cleaned sponge skeleton can be used to culture iPSC-CMs and 3D tissue engineering. In addition, I. labyrinthus chitin-based scaffolds demonstrate strong and efficient capability to absorb blood deep into the microtubes due to their excellent capillary effect. These findings are suggestive of the future development of new sponge chitin-based absorbable hemostats as alternatives to already well recognized cellulose-based fabrics.

Collagen/chitosan hybrid 3D-scaffolds as potential biomaterials for tissue engineering

2018 ◽  
Vol 7 (3) ◽  
pp. 163
Author(s):  
Hilary Ureña Saborio ◽  
Emilia Alfaro Viquez ◽  
Daniel Esquivel Alvarado ◽  
Marianelly Esquivel ◽  
Sergio Madrigal Carballo
Keyword(s):  
Tissue Engineering ◽  
3D Scaffolds
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