Co-delivery of Simvastatin and Demineralized Bone Matrix Hierarchically from Nanosheet-based Supramolecular Hydrogels for Osteogenesis

2021 ◽  
Author(s):  
Xiao Zhang ◽  
Jiabing Fan ◽  
Chen Chen ◽  
Tara Aghaloo ◽  
Min Lee
Keyword(s):  
Tissue Engineering ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Therapeutic Agents ◽  
Engineering Applications ◽  
3D Scaffolds ◽  
Hydrogel Scaffolds ◽  
Delivery Platform ◽  
Weak Compatibility ◽  
Demineralized Bone

Supramolecular hydrogels are widely used as 3D scaffolds and delivery platform in tissue engineering applications. However, hydrophobic therapeutic agents exhibit weak compatibility in hydrogel scaffolds along with aggregation and precipitate....

Tissue Engineering of Bone by Osteoinductive Biomaterials

MRS Bulletin ◽  
1996 ◽  
Vol 21 (11) ◽  
pp. 36-39 ◽  
Author(s):  
Ugo Ripamonti ◽  
Nicolaas Duneas
Keyword(s):  
Tissue Engineering ◽  
Bone Formation ◽  
Type I Collagen ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Mesenchymal Cells ◽  
Tissue Response ◽  
Type I ◽  
New Bone Formation ◽  
Demineralized Bone

Recent advances in materials science and biotechnology have given birth to the new and exciting field of tissue engineering, in which the two normally disparate fields are merging into a profitable matrimony. In particular the use of biomaterials capable of initiating new bone formation via a process called osteoinduction is leading to quantum leaps for the tissue engineering of bone.The classic work of Marshall R. Urist and A. Hari Reddi opened the field of osteoinductive biomaterials. Urist discovered that, upon implantation of devitalized, demineralized bone matrix in the muscle of experimental animals, new bone formation occurs within two weeks, a phenomenon he described as bone formation by induction. The tissue response elicited by implantation of demineralized bone matrix in muscle or under the skin includes activation and migration of undifferentiated mesenchymal cells by chemotaxis, anchoragedependent cell attachment to the matrix, mitosis and proliferation of mesenchymal cells, differentiation of cartilage, mineralization of the cartilage, vascular invasion of the cartilage, differentiation of osteoblasts and deposition of bone matrix, and finally mineralization of bone and differentiation of marrow in the newly developed ossicle.The osteoinductive ability of the extracellular matrix of bone is abolished by the dissociative extraction of the demineralized matrix, but is recovered when the extracted component, itself inactive, is reconstituted with the inactive residue—mainly insoluble collagenous bone matrix. This important experiment showed that the osteoinductive signal resides in the solubilized component but needs to be reconstituted with an appropriate carrier to restore the osteoinductive activity. In this case, the carrier is the insoluble collagenous bone matrix—mainly crosslinked type I collagen.

Cartilage tissue engineering of nasal septal chondrocyte-macroaggregates in human demineralized bone matrix

2012 ◽  
Vol 14 (2) ◽  
pp. 255-266 ◽  
Author(s):  
Juliane Liese ◽  
Ulrike Marzahn ◽  
Karym El Sayed ◽  
Axel Pruss ◽  
Andreas Haisch ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Demineralized Bone

Multipotent adult progenitor cell-loaded demineralized bone matrix for bone tissue engineering

2013 ◽  
Vol 10 (4) ◽  
pp. 275-283 ◽  
Author(s):  
Peter Supronowicz ◽  
Elise Gill ◽  
Angelica Trujillo ◽  
Taili Thula ◽  
Rasa Zhukauskas ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Progenitor Cell ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Multipotent Adult Progenitor Cell ◽  
Demineralized Bone

Human Adipose-Derived Side Population Stem Cells Cultured on Demineralized Bone Matrix for Bone Tissue Engineering

2011 ◽  
Vol 17 (5-6) ◽  
pp. 789-798 ◽  
Author(s):  
Peter Supronowicz ◽  
Elise Gill ◽  
Angelica Trujillo ◽  
Taili Thula ◽  
Rasa Zhukauskas ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Side Population ◽  
Bone Matrix ◽  
Cells Cultured ◽  
Demineralized Bone

scholarly journals In Vitro Comparative Study of Osteogenic Differentiation Ability between Adipose and Bone Marrow Mesenchymal Stem Cell Applied to Bovine Demineralized Bone Matrix

2018 ◽  
Vol 38 ◽  
pp. 59-66 ◽  
Author(s):  
Ferdiansyah Mahyudin ◽  
Dwikora Novembri Utomo ◽  
Mouli Edward ◽  
Lukas Widhiyanto ◽  
Christopher Anthony Simanjuntak ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Zealand White Rabbit ◽  
Tissue Engineering Application ◽  
Alkaline Phosphate ◽  
Demineralized Bone

Ideal bone graft must possess the desirable trait such as osteoconductive, osteoinductive and osteogenesis. Demineralized Bone Matrix (DBM) provides both osteoconductive and osteoinductive trait. Referring to the tissue engineering principle, the addition of mesenchymal stem cell would add the osteogenic trait to this procedure. The design of this study is experimental using Bovine DBM. Bone Marrow Mesenchymal Stem Cell (BMSCs) and Adipose Mesenchymal Stem Cells (ASCs) were taken from New Zealand white rabbit. There are two groups of treatment, divided into DBM implanted with BMSCs and DBM implanted with ASCs. Each BMSCs and ASCs groups is incubated in the normal and osteogenic culture plate. Evaluation is performed by counting the osteoblast and immunohistochemistry stain using Alkaline Phosphate and Osteocalcin. After 4 weeks of incubation, we found that the osteoblast count in BMSCs groups is higher compared to the ASCs groups in both culture condition (p<0.01) along with Alkaline Phosphate staining (p<0.05), while the Osteocalcin staining showed insignificant differences (p>0.05). This study revealed that xenogenic bovine DBM can act as the potential osteoinductive scaffold for the MSCs to differentiate. The tissue engineering application by combining MSCs and Bovine DBM can be considered as an alternative in managing bone defect cases.

Demineralized bone matrix gelatin as scaffold for osteochondral tissue engineering

Biomaterials ◽  
2006 ◽  
Vol 27 (11) ◽  
pp. 2426-2433 ◽  
Author(s):  
Xudong Li ◽  
Li Jin ◽  
Gary Balian ◽  
Cato T. Laurencin ◽  
D. Greg Anderson
Keyword(s):  
Tissue Engineering ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Osteochondral Tissue ◽  
Osteochondral Tissue Engineering ◽  
Demineralized Bone
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