In vitro and in vivo Diagnosis of Pulmonary Disorders Using Nanotechnology

2012 ◽  
pp. 103-133
Author(s):  
Indrajit Roy
Keyword(s):  
In Vivo Diagnosis

Quantification of Formation and Remineralization of Artificial Enamel Lesions with a New Portable Fluorescence Device

1997 ◽  
Vol 11 (4) ◽  
pp. 502-506 ◽  
Author(s):  
S. Al-Khateeb ◽  
J.M. Ten Cate ◽  
B. Angmar-Månsson ◽  
E. De Josselin De Jong ◽  
G. Sundström ◽  
...  
Keyword(s):  
Laser Light ◽  
Laser System ◽  
Laser Fluorescence ◽  
New Device ◽  
Portable System ◽  
In Vivo Diagnosis ◽  
Non Destructive ◽  
Mineral Loss

Quantitative laser fluorescence has been reported as a useful method for the non-destructive in vitro and in vivo diagnosis of early enamel caries. A portable system for intraoral use has been developed with a new light source and filter system replacing the laser light to facilitate clinical application. This new device was validated with microradiographic and chemical analyses for assessment of mineral changes in enamel during lesion formation and remineralization in vitro and compared with the laser light equipment. A significant correlation was found between fluorescence changes and mineral loss: r = 0.79 (laser system) and r = 0.84 (portable lamp system). The correlation between the two fluorescence methods was r = 0.93. The portable fluorescence device seemed to be a promising new tool for reproducible and sensitive assesment of the severity of incipient enamel lesions.

scholarly journals Metastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Estefanía Sicco ◽  
Amy Mónaco ◽  
Marcelo Fernandez ◽  
María Moreno ◽  
Victoria Calzada ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Metastatic Melanoma ◽  
Dna Aptamer ◽  
Specific Expression ◽  
Molecular Imaging Probes ◽  
Imaging Probes ◽  
Melanoma Model ◽  
In Vivo Diagnosis

AbstractMelanoma is one of the most aggressive and deadly skin cancers, and although histopathological criteria are used for its prognosis, biomarkers are necessary to identify the different evolution stages. The applications of molecular imaging include the in vivo diagnosis of cancer with probes that recognize the tumor-biomarkers specific expression allowing external image acquisitions and evaluation of the biological process in quali-quantitative ways. Aptamers are oligonucleotides that recognize targets with high affinity and specificity presenting advantages that make them interesting molecular imaging probes. Sgc8-c (DNA-aptamer) selectively recognizes PTK7-receptor overexpressed in various types of tumors. Herein, Sgc8-c was evaluated, for the first time, in a metastatic melanoma model as molecular imaging probe for in vivo diagnostic, as well as in a non-metastatic melanoma model. Firstly, two probes, radio- and fluorescent-probe, were in vitro evaluated verifying the high specific PTK7 recognition and its internalization in tumor cells by the endosomal route. Secondly, in vivo proof of concept was performed in animal tumor models. In addition, they have rapid clearance from blood exhibiting excellent target (tumor)/non-target organ ratios. Furthermore, optimal biodistribution was observed 24 h after probes injections accumulating almost exclusively in the tumor tissue. Sgc8-c is a potential tool for their specific use in the early detection of melanoma.

scholarly journals Oral allergy syndrome by fruit and vegetable PR-10 allergy: Accuracy of in vivo diagnosis

2021 ◽  
Vol 49 (2) ◽  
pp. 129-132
Author(s):  
Cristina De Rose ◽  
Maria Letitzia Patti ◽  
Alessadro Gambacorta ◽  
Federica Brancato ◽  
Stefano Miceli Sopo
Keyword(s):  
Specific Ige ◽  
Fruit And Vegetables ◽  
Diagnostic Methods ◽  
Oral Allergy Syndrome ◽  
In Vitro Tests ◽  
Prick Test ◽  
Fresh Food ◽  
In Vivo Diagnosis

Routine diagnostic methods for allergies to plant-derived foods are based on skin prick test (SPT) with commercial extracts, prick-by-prick (PbP) with fresh food, serum-specific IgE measurement, and oral food challenge.We discuss the possibility and the advantages of performing, in patients with oral allergy syn-drome (OAS) by fruit and vegetables (excluding nuts) PR-10 allergy, component-resolved diag-nosis (CRD) by SPT and PbP with raw and cooked vegetables, rather than performing a CRD with in vitro tests by drawing blood.Based on our clinical experience and the studies published in the literature, we believe that, at least for the OAS by fruit and vegetables (excluding nuts) PR-10 allergy, the search for sensitizing allergens and related cross-reactive allergens with SPT and PbP can be performed routinely in clinical practice, even at the primary-care level.

Generation of Allergen-Enriched Protein Fractions of Hevea brasiliensis Latex for in vitro and in vivo Diagnosis

2007 ◽  
Vol 143 (4) ◽  
pp. 246-254 ◽  
Author(s):  
S. Wagner ◽  
M. Bublin ◽  
C. Hafner ◽  
T. Kopp ◽  
D. Allwardt ◽  
...  
Keyword(s):  
Hevea Brasiliensis ◽  
Protein Fractions ◽  
In Vivo Diagnosis
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