Applicability of photoacoustic measurement for biomechanical characterization: from in vitro engineered tissue characterization to in vivo diagnosis

2004 ◽  
Author(s):  
Miya Ishihara ◽  
Masato Sato ◽  
Shunichi Sato ◽  
Toshiyuki Kikuchi ◽  
Masayuki Ishihara ◽  
...  
Keyword(s):  
Tissue Characterization ◽  
Engineered Tissue ◽  
Photoacoustic Measurement ◽  
In Vivo Diagnosis

scholarly journals Non-adhesive alginate hydrogels support growth of pluripotent stem cell-derived intestinal organoids

2018 ◽  
Author(s):  
Meghan M. Capeling ◽  
Michael Czerwinski ◽  
Sha Huang ◽  
Yu-Hwai Tsai ◽  
Angeline Wu ◽  
...  
Keyword(s):  
Pluripotent Stem Cell ◽  
Screening Tools ◽  
Similar Degree ◽  
Adhesion Properties ◽  
Intestinal Organoids ◽  
The Matrix ◽  
Survival And Development ◽  
Engineered Tissue

SummaryHuman intestinal organoids (HIOs) represent a powerful system to study human development and are promising candidates for clinical translation as drug-screening tools or engineered tissue. Experimental control and clinical use of HIOs is limited by growth in expensive and poorly defined tumor-cell-derived extracellular matrices, prompting investigation of synthetic ECM-mimetics for HIO culture. Since HIOs possess an inner epithelium and outer mesenchyme, we hypothesized that adhesive cues provided by the matrix may be dispensable for HIO culture. Here, we demonstrate that alginate, a minimally supportive hydrogel with no inherent cell adhesion properties, supports HIO growth in vitro and leads to HIO epithelial differentiation that is virtually indistinguishable from Matrigel-grown HIOs. Additionally, alginate-grown HIOs mature to a similar degree as Matrigel-grown HIOs when transplanted in vivo, both resembling human fetal intestine. This work demonstrates that purely mechanical support from a simple-to-use and inexpensive hydrogel is sufficient to promote HIO survival and development.

scholarly journals Development of human ectocervical tissue models with physiologic endocrine and paracrine signaling†

2020 ◽  
Vol 103 (3) ◽  
pp. 497-507 ◽  
Author(s):  
Kelly E McKinnon ◽  
Rhitwika Sensharma ◽  
Chloe Williams ◽  
Jovanka Ravix ◽  
Spiro Getsios ◽  
...  
Keyword(s):  
Hormone Action ◽  
Paracrine Signaling ◽  
Mucin Production ◽  
Decellularized Extracellular Matrix ◽  
Fundamental Research ◽  
Engineered Tissue ◽  
Tissue Models ◽  
First Time

Abstract There is a shortage of research models that adequately represent the unique mucosal environment of human ectocervix, limiting development of new therapies for treating infertility, infection, or cancer. We developed three microphysiologic human ectocervix models to study hormone action during homeostasis. First, we reconstructed ectocervix using decellularized extracellular matrix scaffolds, which supported cell integration and could be clinically useful. Secondly, we generated organotypic systems consisting of ectocervical explants co-cultured with murine ovaries or cycling exogenous hormones, which mimicked human menstrual cycles. Finally, we engineered ectocervix tissue consisting of tissue-specific stromal-equivalents and fully-differentiated epithelium that mimicked in vivo physiology, including squamous maturation, hormone response, and mucin production, and remained viable for 28 days in vitro. The localization of differentiation-dependent mucins in native and engineered tissue was identified for the first time, which will allow increased efficiency in mucin targeting for drug delivery. In summary, we developed and characterized three microphysiologic human ectocervical tissue models that will be useful for a variety of research applications, including preventative and therapeutic treatments, drug and toxicology studies, and fundamental research on hormone action in a historically understudied tissue that is critical for women’s health.

A 120-MHz Ultrasound Probe for Tissue Imaging

1996 ◽  
Vol 18 (4) ◽  
pp. 231-239 ◽  
Author(s):  
Koichi Yokosawa ◽  
Ryuichi Shinomura ◽  
Shyuzo Sano ◽  
Yukio Ito ◽  
Shizuo Ishikawa ◽  
...  
Keyword(s):  
High Frequency ◽  
Tissue Characterization ◽  
Lateral Resolution ◽  
Tissue Imaging ◽  
Surrounding Tissue ◽  
Center Frequency ◽  
Ultrasound Transducers ◽  
High Frequency Region

Ultrasound transducers with center frequency above 100 MHz are expected to be used for future diagnostic tissue characterization because of their high lateral resolution. We have fabricated a 120-MHz transducer that consists of a ZnO piezoelectric film on a sapphire substrate that has a concave acoustic lens. The lateral resolution was calculated as 13 μm. The insertion loss of the transducer, defined as the difference between the received voltage and the transmitted one, was −45 dB. The 6-dB bandwidth of the received signal was approximately 40 MHz. The transducer was mounted in a rod-shaped probe to ensure contact with in vivo tissue, because of the low penetration of ultrasound in the high frequency region. While the probe is rotated and moved along its axis mechanically, the transducer receives backscattered ultrasound from the surrounding tissue on a cylindrical plane that is kept a constant distance from the probe surface. The feasibility of this high-frequency tissue imaging probe has been demonstrated by obtaining preliminary images of an in vitro bovine kidney.

scholarly journals 3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

2020 ◽  
Vol 21 (20) ◽  
pp. 7757
Author(s):  
Jongmin Kim ◽  
Jeong Sik Kong ◽  
Wonil Han ◽  
Byoung Soo Kim ◽  
Dong-Woo Cho
Keyword(s):  
Tissue Engineering ◽  
Drug Testing ◽  
In Vitro Testing ◽  
Living Tissue ◽  
Cell Printing ◽  
Artificial Tissue ◽  
Engineered Tissue ◽  
Pharmaceutical Industries

The development of artificial tissue/organs with the functional maturity of their native equivalents is one of the long-awaited panaceas for the medical and pharmaceutical industries. Advanced 3D cell-printing technology and various functional bioinks are promising technologies in the field of tissue engineering that have enabled the fabrication of complex 3D living tissue/organs. Various requirements for these tissues, including a complex and large-volume structure, tissue-specific microenvironments, and functional vasculatures, have been addressed to develop engineered tissue/organs with native relevance. Functional tissue/organ constructs have been developed that satisfy such criteria and may facilitate both in vivo replenishment of damaged tissue and the development of reliable in vitro testing platforms for drug development. This review describes key developments in technologies and materials for engineering 3D cell-printed constructs for therapeutic and drug testing applications.

Quantification of Formation and Remineralization of Artificial Enamel Lesions with a New Portable Fluorescence Device

1997 ◽  
Vol 11 (4) ◽  
pp. 502-506 ◽  
Author(s):  
S. Al-Khateeb ◽  
J.M. Ten Cate ◽  
B. Angmar-Månsson ◽  
E. De Josselin De Jong ◽  
G. Sundström ◽  
...  
Keyword(s):  
Laser Light ◽  
Laser System ◽  
Laser Fluorescence ◽  
New Device ◽  
Portable System ◽  
In Vivo Diagnosis ◽  
Non Destructive ◽  
Mineral Loss

Quantitative laser fluorescence has been reported as a useful method for the non-destructive in vitro and in vivo diagnosis of early enamel caries. A portable system for intraoral use has been developed with a new light source and filter system replacing the laser light to facilitate clinical application. This new device was validated with microradiographic and chemical analyses for assessment of mineral changes in enamel during lesion formation and remineralization in vitro and compared with the laser light equipment. A significant correlation was found between fluorescence changes and mineral loss: r = 0.79 (laser system) and r = 0.84 (portable lamp system). The correlation between the two fluorescence methods was r = 0.93. The portable fluorescence device seemed to be a promising new tool for reproducible and sensitive assesment of the severity of incipient enamel lesions.

scholarly journals Applications of Engineering Techniques in Microvasculature Design

2021 ◽  
Vol 8 ◽  
Author(s):  
Aleen Al Halawani ◽  
Ziyu Wang ◽  
Linyang Liu ◽  
Miao Zhang ◽  
Anthony S. Weiss
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Capillary Tube ◽  
Tube Formation ◽  
Engineered Tissue ◽  
Post Implantation

Achieving successful microcirculation in tissue engineered constructs in vitro and in vivo remains a challenge. Engineered tissue must be vascularized in vitro for successful inosculation post-implantation to allow instantaneous perfusion. To achieve this, most engineering techniques rely on engineering channels or pores for guiding angiogenesis and capillary tube formation. However, the chosen materials should also exhibit properties resembling the native extracellular matrix (ECM) in providing mechanical and molecular cues for endothelial cells. This review addresses techniques that can be used in conjunction with matrix-mimicking materials to further advance microvasculature design. These include electrospinning, micropatterning and bioprinting. Other techniques implemented for vascularizing organoids are also considered for their potential to expand on these approaches.

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