Generation of Allergen-Enriched Protein Fractions of Hevea brasiliensis Latex for in vitro and in vivo Diagnosis

2007 ◽  
Vol 143 (4) ◽  
pp. 246-254 ◽  
Author(s):  
S. Wagner ◽  
M. Bublin ◽  
C. Hafner ◽  
T. Kopp ◽  
D. Allwardt ◽  
...  
Keyword(s):  
Hevea Brasiliensis ◽  
Protein Fractions ◽  
In Vivo Diagnosis

scholarly journals STUDIES IN IMMUNITY AND ANAPHYLAXIS

1911 ◽  
Vol 14 (1) ◽  
pp. 44-58 ◽  
Author(s):  
Richard M. Pearce ◽  
Howard T. Karsner ◽  
Arthur B. Eisenbrey
Keyword(s):  
Protein Fractions ◽  
Cross Reactions ◽  
Protein Specificity ◽  
In Vivo Experiments ◽  
Liver And Kidney ◽  
Precipitin Test ◽  
Respective Protein

1. The sera of rabbits injected repeatedly with the nucleoproteins, globulins, and albumins of the liver and kidney of the dog give no evidence in vitro or in vivo experiments of organ affinity. The precipitin test offers no proof of the specificity of these sera for the proteins employed as antigens. 2. The anaphylaxis reaction applied to the same proteins indicates a slight relative organ affinity but no specificity as far as the respective protein fractions are concerned. The relative organ affinity resides, rather, in the globulin and albumin fractions than in the nucleoprotein fraction. Dog serum used both as a sensitizing and an intoxicating agent gives rise to very active cross reactions with organ proteins, thus failing to support the theory of organ or of protein specificity. 3. These results do not support the view put forward that nucleoproteins play an important part in the course of production of cytotoxic immune sera.

scholarly journals SOURCES OF LARVAL SALIVARY GLAND SECRETION IN THE DIPTERAN CHIRONOMUS TENTANS

1969 ◽  
Vol 40 (1) ◽  
pp. 61-78 ◽  
Author(s):  
D. Doyle ◽  
H. Laufer
Keyword(s):  
Salivary Gland ◽  
De Novo ◽  
Salivary Secretion ◽  
Disc Electrophoresis ◽  
Protein Fractions ◽  
Chironomus Tentans ◽  
Salivary Gland Secretion ◽  
Larval Salivary Gland

The soluble proteins in the hemolymph, the salivary gland, and the salivary secretion of fourth instar Chironomus tentans were examined by disc electrophoresis in acrylamide gels. Of the 11 protein fractions detected in buffered saline extracts of the gland, 10 are present also in the hemolymph. Amino acid isotope incorporation experiments indicate that the protein fractions shared by the salivary gland and the hemolymph are not synthesized in the gland but are synthesized in other larval tissues. Immunochemical studies show that most of these proteins eventually are secreted from the gland. The salivary gland in vivo and in vitro is active in de novo protein synthesis. The protein synthesized tends to form large molecular weight aggregates. As demonstrated by radioautography, at least 80% of this protein is secreted from the 30 large cells forming most of the gland. The proteins synthesized in the salivary gland cannot be detected in the hemolymph. The results of this investigation are consistent with a mechanism of secretion formation involving both de novo synthesis of some secretion proteins and the selective uptake, transport, and secretion of hemal proteins by the salivary gland.

Protein synthesis in pulmonary arteries from rats exposed to hyperoxia

1993 ◽  
Vol 264 (1) ◽  
pp. L74-L79
Author(s):  
W. S. Stirewalt ◽  
J. T. Coflesky ◽  
L. H. Young ◽  
J. N. Evans
Keyword(s):  
Protein Synthesis ◽  
Soluble Protein ◽  
Growth Response ◽  
Pulmonary Arteries ◽  
Protein Fractions ◽  
Tissue Protein ◽  
Fractional Rate ◽  
Fractional Synthesis

These studies were undertaken to determine the relationship of early changes in the synthesis rates and contents of collagen, elastin, and soluble tissue protein of pulmonary arteries in rats exposed chronically to normobaric hyperoxia. The growth response of pulmonary arteries was characterized by proportionate increases in the contents of the three protein fractions after 7 days (130% of control) and 21 days (194% of control) of exposure. Fractional rates of protein synthesis were assessed both in vivo and in vitro with the use of several radiolabeled amino acids as tracers to minimize uncertainties of the relationships of the specific radioactivities of measured amino acid pools and the precursors for the proteins fractions. Values for fractional synthesis rates of collagen, elastin, and soluble protein in vitro in pulmonary arteries isolated from control rats were 2.2, 1.6, and 19%/day, respectively. Rates of synthesis of collagen and soluble protein in vitro were approximately 20% lower than that determined in control rats in vivo. The fractional synthesis rates of the three protein fractions in isolated arteries from experimental rats were unchanged after 1 day of hyperoxic exposure, decreased marginally after 3 days, and markedly increased after 7 days. At this time the absolute increments in the fractional synthesis rates of collagen (+4.7%/day) and elastin (+5.0%/day) were less than that of soluble tissue protein (+16%/day) and were more comparable to the accumulation rate of proteins in the tissue. The disproportionate increment in the fractional rate of soluble protein synthesis suggests that the fractional rate of degradation of soluble protein was also increased during the growth response in this model of hypertension.

Quantification of Formation and Remineralization of Artificial Enamel Lesions with a New Portable Fluorescence Device

1997 ◽  
Vol 11 (4) ◽  
pp. 502-506 ◽  
Author(s):  
S. Al-Khateeb ◽  
J.M. Ten Cate ◽  
B. Angmar-Månsson ◽  
E. De Josselin De Jong ◽  
G. Sundström ◽  
...  
Keyword(s):  
Laser Light ◽  
Laser System ◽  
Laser Fluorescence ◽  
New Device ◽  
Portable System ◽  
In Vivo Diagnosis ◽  
Non Destructive ◽  
Mineral Loss

Quantitative laser fluorescence has been reported as a useful method for the non-destructive in vitro and in vivo diagnosis of early enamel caries. A portable system for intraoral use has been developed with a new light source and filter system replacing the laser light to facilitate clinical application. This new device was validated with microradiographic and chemical analyses for assessment of mineral changes in enamel during lesion formation and remineralization in vitro and compared with the laser light equipment. A significant correlation was found between fluorescence changes and mineral loss: r = 0.79 (laser system) and r = 0.84 (portable lamp system). The correlation between the two fluorescence methods was r = 0.93. The portable fluorescence device seemed to be a promising new tool for reproducible and sensitive assesment of the severity of incipient enamel lesions.

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