Nanogravimetric and Voltammetric DNA-Biosensors for Screening of Herbicides and Pesticides

2012 ◽  
pp. 246-266
Keyword(s):  
Dna Biosensors

scholarly journals Review of Electrochemical DNA Biosensors for Detecting Food Borne Pathogens

Sensors ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 4916 ◽  
Author(s):  
Qiaoyun Wu ◽  
Yunzhe Zhang ◽  
Qian Yang ◽  
Ning Yuan ◽  
Wei Zhang
Keyword(s):  
Pathogen Detection ◽  
Low Cost ◽  
Electrochemical Techniques ◽  
Ease Of Use ◽  
Detection Methods ◽  
Future Research ◽  
Dna Biosensors ◽  
Food Borne Pathogens ◽  
Chip Technology ◽  
Food Borne

The vital importance of rapid and accurate detection of food borne pathogens has driven the development of biosensor to prevent food borne illness outbreaks. Electrochemical DNA biosensors offer such merits as rapid response, high sensitivity, low cost, and ease of use. This review covers the following three aspects: food borne pathogens and conventional detection methods, the design and fabrication of electrochemical DNA biosensors and several techniques for improving sensitivity of biosensors. We highlight the main bioreceptors and immobilizing methods on sensing interface, electrochemical techniques, electrochemical indicators, nanotechnology, and nucleic acid-based amplification. Finally, in view of the existing shortcomings of electrochemical DNA biosensors in the field of food borne pathogen detection, we also predict and prospect future research focuses from the following five aspects: specific bioreceptors (improving specificity), nanomaterials (enhancing sensitivity), microfluidic chip technology (realizing automate operation), paper-based biosensors (reducing detection cost), and smartphones or other mobile devices (simplifying signal reading devices).

Approaches to label-free flexible DNA biosensors using low-temperature solution-processed InZnO thin-film transistors

2014 ◽  
Vol 55 ◽  
pp. 99-105 ◽  
Author(s):  
Joohye Jung ◽  
Si Joon Kim ◽  
Keun Woo Lee ◽  
Doo Hyun Yoon ◽  
Yeong-gyu Kim ◽  
...  
Keyword(s):  
Thin Film ◽  
Low Temperature ◽  
Thin Film Transistors ◽  
Label Free ◽  
Dna Biosensors ◽  
Solution Processed ◽  
Temperature Solution

Surface regeneration and reusability of label-free DNA biosensors based on weak polyelectrolyte-modified capacitive field-effect structures

2019 ◽  
Vol 126 ◽  
pp. 510-517 ◽  
Author(s):  
Thomas S. Bronder ◽  
Arshak Poghossian ◽  
Max P. Jessing ◽  
Michael Keusgen ◽  
Michael J. Schöning
Keyword(s):  
Field Effect ◽  
Label Free ◽  
Dna Biosensors ◽  
Free Dna ◽  
Surface Regeneration

Enhancing the Photoelectrochemical Response of DNA Biosensors Using Wrinkled Interfaces

2018 ◽  
Vol 10 (37) ◽  
pp. 31178-31185 ◽  
Author(s):  
Sudip Saha ◽  
Yuting Chan ◽  
Leyla Soleymani
Keyword(s):  
Dna Biosensors ◽  
Photoelectrochemical Response

scholarly journals Highly Sensitive Detection of Campylobacter spp. in Chicken Meat using a Silica Nanoparticle Enhanced Dot Blot DNA Biosensor

2020 ◽  
Author(s):  
Priya Vizzini ◽  
Marisa Manzano ◽  
Carole Farre ◽  
Thierry Meylheuc ◽  
Carole Chaix ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Silica Nanoparticle ◽  
Chicken Meat ◽  
Dot Blot ◽  
Dna Biosensors ◽  
Point Of Care Diagnostics ◽  
Detection Probe ◽  
Highly Sensitive Detection ◽  
User Friendly

AbstractPaper-based DNA biosensors are powerful tools in point-of-care diagnostics since they are affordable, portable, user-friendly, rapid and robust. However, their sensitivity is not always as high as required to enable DNA quantification. To improve the response of standard dot blots, we have applied a new enhancement strategy that increases the sensitivity of assays based on the use of biotinylated silica-nanoparticles (biotin-Si-NPs). After immobilization of a genomic Campylobacter DNA onto a paper membrane, and addition of a biotinylated-DNA detection probe, hybridization was evidenced using streptavidin-conjugated to horseradish peroxidase (HRP) in the presence of luminol and H2O2. Replacement of the single biotin by the biotin-Si-NPs boosted on average a 30 fold chemiluminescent read-out of the biosensor. Characterization of biotin-Si-NPs onto a paper with immobilized DNA was done using a scanning electron microscope. A limit of detection of 3 pg/μL of DNA, similar to the available qPCR kits, is achieved, but it is cheaper, easier and avoids inhibition of DNA polymerase by molecules from the food matrices. We demonstrated that the new dot blot coupled to biotin-Si-NPs successfully detected Campylobacter from naturally contaminated chicken meat, without needing a PCR step. Hence, such an enhanced dot blot paves the path to the development of a portable and multiplex paper based platform for point-of-care screening of chicken carcasses for Campylobacter.Graphical abstract

scholarly journals Characterizing Metabolic Inhibition Using Electrochemical Enzyme/DNA Biosensors

2009 ◽  
Vol 81 (2) ◽  
pp. 716-724 ◽  
Author(s):  
Dominic O. Hull ◽  
Besnik Bajrami ◽  
Ingela Jansson ◽  
John B. Schenkman ◽  
James F. Rusling
Keyword(s):  
Metabolic Inhibition ◽  
Dna Biosensors

Deoxyribonucleic acid (DNA) biosensors for environmental risk assessment and drug studies

2006 ◽  
Vol 573-574 ◽  
pp. 81-89 ◽  
Author(s):  
Graziana Bagni ◽  
Domenico Osella ◽  
Elena Sturchio ◽  
Marco Mascini
Keyword(s):  
Risk Assessment ◽  
Environmental Risk ◽  
Environmental Risk Assessment ◽  
Deoxyribonucleic Acid ◽  
Dna Biosensors ◽  
Drug Studies
Sign in / Sign up

Export Citation Format

Share Document