scholarly journals Characterizing Metabolic Inhibition Using Electrochemical Enzyme/DNA Biosensors

2009 ◽  
Vol 81 (2) ◽  
pp. 716-724 ◽  
Author(s):  
Dominic O. Hull ◽  
Besnik Bajrami ◽  
Ingela Jansson ◽  
John B. Schenkman ◽  
James F. Rusling
Keyword(s):  
Metabolic Inhibition ◽  
Dna Biosensors

scholarly journals Review of Electrochemical DNA Biosensors for Detecting Food Borne Pathogens

Sensors ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 4916 ◽  
Author(s):  
Qiaoyun Wu ◽  
Yunzhe Zhang ◽  
Qian Yang ◽  
Ning Yuan ◽  
Wei Zhang
Keyword(s):  
Pathogen Detection ◽  
Low Cost ◽  
Electrochemical Techniques ◽  
Ease Of Use ◽  
Detection Methods ◽  
Future Research ◽  
Dna Biosensors ◽  
Food Borne Pathogens ◽  
Chip Technology ◽  
Food Borne

The vital importance of rapid and accurate detection of food borne pathogens has driven the development of biosensor to prevent food borne illness outbreaks. Electrochemical DNA biosensors offer such merits as rapid response, high sensitivity, low cost, and ease of use. This review covers the following three aspects: food borne pathogens and conventional detection methods, the design and fabrication of electrochemical DNA biosensors and several techniques for improving sensitivity of biosensors. We highlight the main bioreceptors and immobilizing methods on sensing interface, electrochemical techniques, electrochemical indicators, nanotechnology, and nucleic acid-based amplification. Finally, in view of the existing shortcomings of electrochemical DNA biosensors in the field of food borne pathogen detection, we also predict and prospect future research focuses from the following five aspects: specific bioreceptors (improving specificity), nanomaterials (enhancing sensitivity), microfluidic chip technology (realizing automate operation), paper-based biosensors (reducing detection cost), and smartphones or other mobile devices (simplifying signal reading devices).

Approaches to label-free flexible DNA biosensors using low-temperature solution-processed InZnO thin-film transistors

2014 ◽  
Vol 55 ◽  
pp. 99-105 ◽  
Author(s):  
Joohye Jung ◽  
Si Joon Kim ◽  
Keun Woo Lee ◽  
Doo Hyun Yoon ◽  
Yeong-gyu Kim ◽  
...  
Keyword(s):  
Thin Film ◽  
Low Temperature ◽  
Thin Film Transistors ◽  
Label Free ◽  
Dna Biosensors ◽  
Solution Processed ◽  
Temperature Solution

Effect of Glibenclamide on Membrane Response to Metabolic Inhibition in Smooth Muscle of Rat Portal Vein

1994 ◽  
Vol 31 (2) ◽  
pp. 82-91 ◽  
Author(s):  
Marie-Louise Lydrup ◽  
Karl Swärd ◽  
Per Hellstrand
Keyword(s):  
Smooth Muscle ◽  
Portal Vein ◽  
Metabolic Inhibition ◽  
Rat Portal Vein

Surface regeneration and reusability of label-free DNA biosensors based on weak polyelectrolyte-modified capacitive field-effect structures

2019 ◽  
Vol 126 ◽  
pp. 510-517 ◽  
Author(s):  
Thomas S. Bronder ◽  
Arshak Poghossian ◽  
Max P. Jessing ◽  
Michael Keusgen ◽  
Michael J. Schöning
Keyword(s):  
Field Effect ◽  
Label Free ◽  
Dna Biosensors ◽  
Free Dna ◽  
Surface Regeneration

scholarly journals Serological tests for the detection of antibodies to Mycoplasma gallisepticum in chickens and turkeys

1968 ◽  
Vol 66 (2) ◽  
pp. 249-267 ◽  
Author(s):  
F. T. W. Jordan ◽  
P. Kulasegaram
Keyword(s):  
Positive Reaction ◽  
Complement Fixation ◽  
Haemagglutination Inhibition ◽  
Mycoplasma Gallisepticum ◽  
Metabolic Inhibition ◽  
Agar Gel ◽  
Serological Tests ◽  
Anamnestic Response ◽  
Initial Infection ◽  
Gel Method

SUMMARYA comparison was undertaken of several serological tests in determining the response of chickens and turkeys experimentally infected with the A 514 strain of Mycoplasma gallisepticum.After a single intratracheal inoculation of chickens with a culture of the organism, the highest titres were obtained by the indirect complement fixation (ICF) test, followed by the tube agglutination (TA), haemagglutination inhibition (HI), slide agglutination (SA) and metabolic inhibition (MI) tests. By all these tests positive titres were observed within the first week and peak titres between the first and second weeks. At 5 months there was no positive reaction by the ICF test but most chickens gave positive readings by the TA, HI and SA tests for at least 14 months after infection, but turkey sera became negative by all tests after 3 months.A disadvantage of the ICF test was that sera up to a dilution of 1/8 and 1/16 for chicken and turkey respectively were anticomplementary, and in turkeys this masked the ICF titre, which presumably was low following one intratracheal inoculation. Titres in turkeys with the TA, HI and SA tests followed the pattern seen with chickens and were generally lower than those found by other workers probably because of the avirulent nature of the inoculum used.The WB test was the least sensitive of the agglutination tests but is useful as a flock test which can be undertaken on the farm.The MI test gave the lowest titres of all and antibodies could be detected for only 4 months following one intratracheal inoculation. Even with serum prepared by multiple inoculations in chickens the titre was never higher than 1/32 compared with 1/1024 for serum similarly prepared in rabbits.Precipitins were detected by the agar gel method in the sera of chickens and turkeys after two intratracheal inoculations but in only some of the chickens and none of the turkeys after one inoculation.By all tests higher titres were observed with chicken than turkey sera and antibodies persisted for a longer time.Re-infection of chickens when antibodies to the initial infection had become low, and of turkeys when antibodies were no longer detectable, gave rise to an anamnestic response with titres which were higher than before.Antiserum to M. gallisepticum prepared in chickens is comparable with that prepared in rabbits except for low titres by the MI test.

Enhancing the Photoelectrochemical Response of DNA Biosensors Using Wrinkled Interfaces

2018 ◽  
Vol 10 (37) ◽  
pp. 31178-31185 ◽  
Author(s):  
Sudip Saha ◽  
Yuting Chan ◽  
Leyla Soleymani
Keyword(s):  
Dna Biosensors ◽  
Photoelectrochemical Response

scholarly journals Nonanticoagulant heparin reduces myocyte Na+ and Ca2+ loading during simulated ischemia and decreases reperfusion injury

2010 ◽  
Vol 298 (1) ◽  
pp. H102-H111 ◽  
Author(s):  
William H. Barry ◽  
Xiu Q. Zhang ◽  
Michael E. Halkos ◽  
Jakob Vinten-Johansen ◽  
Noriko Saegusa ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ventricular Myocytes ◽  
Myocardial Necrosis ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Metabolic Inhibition ◽  
Early Reperfusion ◽  
Simulated Ischemia ◽  
Sea Anemone Toxin ◽  
Voltage Clamping

Heparin desulfated at the 2- O and 3- O positions (ODSH) decreases canine myocardial reperfusion injury. We hypothesized that this occurs from effects on ion channels rather than solely from anti-inflammatory activities, as previously proposed. We studied closed-chest pigs with balloon left anterior descending coronary artery occlusion (75-min) and reperfusion (3-h). ODSH effects on [Na+]i (Na Green) and [Ca2+]i (Fluo-3) were measured by flow cytometry in rabbit ventricular myocytes after 45-min of simulated ischemia [metabolic inhibition with 2 mM cyanide, 0 glucose, 37°C, pacing at 0.5 Hz; i.e., pacing-metabolic inhibition (PMI)]. Na+/Ca2+ exchange (NCX) activity and Na+ channel function were assessed by voltage clamping. ODSH (15 mg/kg) 5 min before reperfusion significantly decreased myocardial necrosis, but neutrophil influx into reperfused myocardium was not consistently reduced. ODSH (100 μg/ml) reduced [Na+]i and [Ca2+]i during PMI. The NCX inhibitor KB-R7943 (10 μM) or the late Na+ current ( INa-L) inhibitor ranolazine (10 μM) reduced [Ca2+]i during PMI and prevented effects of ODSH on Ca2+ loading. ODSH also reduced the increase in Na+ loading in paced myocytes caused by 10 nM sea anemone toxin II, a selective activator of INa-L. ODSH directly stimulated NCX and reduced INa-L. These results suggest that in the intact heart ODSH reduces Na+ influx during early reperfusion, when INa-L is activated by a burst of reactive oxygen production. This reduces Na+ overload and thus Ca2+ influx via NCX. Stimulation of Ca2+ extrusion via NCX later after reperfusion may also reduce myocyte Ca2+ loading and decrease infarct size.

scholarly journals Highly Sensitive Detection of Campylobacter spp. in Chicken Meat using a Silica Nanoparticle Enhanced Dot Blot DNA Biosensor

2020 ◽  
Author(s):  
Priya Vizzini ◽  
Marisa Manzano ◽  
Carole Farre ◽  
Thierry Meylheuc ◽  
Carole Chaix ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Silica Nanoparticle ◽  
Chicken Meat ◽  
Dot Blot ◽  
Dna Biosensors ◽  
Point Of Care Diagnostics ◽  
Detection Probe ◽  
Highly Sensitive Detection ◽  
User Friendly

AbstractPaper-based DNA biosensors are powerful tools in point-of-care diagnostics since they are affordable, portable, user-friendly, rapid and robust. However, their sensitivity is not always as high as required to enable DNA quantification. To improve the response of standard dot blots, we have applied a new enhancement strategy that increases the sensitivity of assays based on the use of biotinylated silica-nanoparticles (biotin-Si-NPs). After immobilization of a genomic Campylobacter DNA onto a paper membrane, and addition of a biotinylated-DNA detection probe, hybridization was evidenced using streptavidin-conjugated to horseradish peroxidase (HRP) in the presence of luminol and H2O2. Replacement of the single biotin by the biotin-Si-NPs boosted on average a 30 fold chemiluminescent read-out of the biosensor. Characterization of biotin-Si-NPs onto a paper with immobilized DNA was done using a scanning electron microscope. A limit of detection of 3 pg/μL of DNA, similar to the available qPCR kits, is achieved, but it is cheaper, easier and avoids inhibition of DNA polymerase by molecules from the food matrices. We demonstrated that the new dot blot coupled to biotin-Si-NPs successfully detected Campylobacter from naturally contaminated chicken meat, without needing a PCR step. Hence, such an enhanced dot blot paves the path to the development of a portable and multiplex paper based platform for point-of-care screening of chicken carcasses for Campylobacter.Graphical abstract

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