Novel Electrochemical DNA Biosensors as Tools for Investigation and Detection of DNA Damage

2016 ◽  
pp. 203-221 ◽  
Author(s):  
Vlastimil Vyskočil ◽  
Andrea Hájková
Keyword(s):  
Dna Damage ◽  
Dna Biosensors

Detection of DNA Damage Induced by Hydroquinone and Catechol Using an Electrochemical DNA Biosensor

2009 ◽  
Vol 62 (9) ◽  
pp. 1181 ◽  
Author(s):  
Chuanhu Wang ◽  
Jun Zhao ◽  
Dapeng Zhang ◽  
Zhousheng Yang
Keyword(s):  
Dna Damage ◽  
Electrochemical Method ◽  
Differential Pulse ◽  
Acetate Buffer Solution ◽  
Dna Biosensors ◽  
Buffer Solution ◽  
Calf Thymus ◽  
Time Differential ◽  
Constant Potential ◽  
Anodic Peak Current

DNA damage induced by hydroquinone and catechol was detected by an electrochemical method. Calf thymus DNA was immobilized onto the surface of a pretreated glassy carbon electrode (GCE(ox)) to form a DNA/GCE(ox) modified electrode. Then the DNA/GCE(ox) was incubated in acetate buffer solution containing hydroquinone or catechol at a constant potential for the desired time. Differential pulse voltammetric experiments were then performed. The anodic peaks corresponding to the oxidation of guanisine and adenosine on the electrode could be observed on voltammetric curves. The experimental results showed that DNA damage could be detected using electrochemical DNA biosensors. The extent of DNA damage could be electrochemically recognized via the change of the anodic peak current. DNA damage induced by hydroquinone was greater than that by catechol. The response conditions were optimized with respect to DNA concentration, pH, ionic strength, and other variables.

1576: Prediction of Spermatozoal DNA Damage by a Novel Semen Quality Score

2004 ◽  
Vol 171 (4S) ◽  
pp. 416-416
Author(s):  
Tamer M. Said ◽  
Shyam Allamaneni ◽  
Kiran P. Nallella ◽  
Rakesh K. Sharma ◽  
Sijo J. Parekattil ◽  
...  
Keyword(s):  
Dna Damage ◽  
Semen Quality ◽  
Quality Score

A safe fix for alcohol-derived DNA damage

Nature ◽  
2020 ◽  
Vol 579 (7800) ◽  
pp. 499-500
Author(s):  
Irene Gallina ◽  
Julien P. Duxin
Keyword(s):  
Dna Damage

Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

1999 ◽  
Vol 96 (1) ◽  
pp. 143-146 ◽  
Author(s):  
J.-P. Pouget ◽  
J.-L. Ravanat ◽  
T. Douki ◽  
M.-J. Richard ◽  
J. Cadet
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Gamma Radiation ◽  
In Cells

Bestimmung der DNA-Schädigung in vitro

2010 ◽  
Vol 49 (S 01) ◽  
pp. S64-S68
Author(s):  
E. Dikomey
Keyword(s):  
Dna Damage ◽  
Cell Lines ◽  
Chromosome Aberrations ◽  
Genetic Factors ◽  
Double Strand Breaks ◽  
Strand Breaks ◽  
Cell Inactivation ◽  
Repair Mechanisms ◽  
Break Repair

SummaryIonising irradiation acts primarily via induction of DNA damage, among which doublestrand breaks are the most important lesions. These lesions may lead to lethal chromosome aberrations, which are the main reason for cell inactivation. Double-strand breaks can be repaired by several different mechanisms. The regulation of these mechanisms appears be fairly different for normal and tumour cells. Among different cell lines capacity of doublestrand break repair varies by only few percents and is known to be determined mostly by genetic factors. Knowledge about doublestrand break repair mechanisms and their regulation is important for the optimal application of ionising irradiation in medicine.

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