Human Liver Development as a Template to Generate High Fidelity Models

2012 ◽  
pp. 38-48
Author(s):  
Claire Medine ◽  
Janet Kung ◽  
Catherine Payne ◽  
James Black ◽  
Richard Anderson ◽  
...  
Keyword(s):  
Human Liver ◽  
Liver Development ◽  
High Fidelity

Matrix metalloproteinases in early human liver development

1999 ◽  
Vol 112 (4) ◽  
pp. 277-282 ◽  
Author(s):  
F. Quondamatteo ◽  
T. Knittel ◽  
M. Mehde ◽  
G. Ramadori ◽  
R. Herken
Keyword(s):  
Matrix Metalloproteinases ◽  
Human Liver ◽  
Liver Development ◽  
Early Human

Identification of proteomic changes during human liver development by 2D-DIGE and mass spectrometry

2009 ◽  
Vol 51 (1) ◽  
pp. 114-126 ◽  
Author(s):  
Jean Paul Brizard ◽  
Jeanne Ramos ◽  
Agnés Robert ◽  
Daniel Lafitte ◽  
Nicole Bigi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Liver ◽  
Liver Development ◽  
2D Dige

Ontogeny of hepatocyte proliferation inhibitor activity during human liver development and its effect on cell proliferation in in vivo and in vitro studies

1993 ◽  
Vol 71 (5-6) ◽  
pp. 241-247 ◽  
Author(s):  
B. Geetha Devi ◽  
C. M. Habeebullah ◽  
P. D. Gupta
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Hepatocyte Proliferation ◽  
Liver Development ◽  
Dodecyl Sulfate

Ontogeny of hepatocyte proliferation inhibitory (HPI) activity was studied during human liver development. HPI activity was first noticed in the cytosolic fraction of 20-week-old fetal liver and thereafter it began to increase with the liver maturation. An inverse correlation between the ontogeny of HPI activity and mitotic counting of the hepatocytes during human liver development was established. The crude HPI fraction from fetal and adult human liver inhibited the incorporation of [3H]thymidine into the DNA of 2-day-old rat liver and HepG2 cells and the inhibitory effect was directly proportional to the age of the HPI source. Prolonged exposure of HepG2 cells to the HPI fraction was also found to be cytotoxic. Preliminary characterization of the crude HPI fraction revealed an age-dependent increase in proteins of 18, 28, and 60 kilodaltons following silver chloride staining of sodium dodecyl sulfate - polyacrylamide gel electrophoresis and densitometric scanning.Key words: human liver, hepatocyte proliferation inhibitor, ontogeny, sodium dodecyl sulfate - polyacrylamide gel electrophoresis, HepG2 cells, cytotoxic.

scholarly journals High resolution, dynamic imaging of early mouse and human liver bud morphogenesis in three dimensions

2019 ◽  
Author(s):  
Tala Mon ◽  
Ogechi Ogoke ◽  
Claire Shamul ◽  
Shatoni Ross ◽  
Saroja Rao ◽  
...  
Keyword(s):  
High Resolution ◽  
Spatial Resolution ◽  
Human Liver ◽  
Disease Modeling ◽  
High Spatial Resolution ◽  
Fetal Liver ◽  
Dynamic Imaging ◽  
Liver Development ◽  
Solid Organ ◽  
Liver Growth

ABSTRACTLiver organogenesis has thus far served as a paradigm for solid organ formation, and has recently attracted interest due to challenges faced in liver regenerative medicine. Murine genetic studies indicate that early steps in morphogenesis are required, suggesting that three-dimensional imaging of early liver morphogenesis at high resolution can improve our understanding. Unfortunately, existing approaches to image early liver morphogenesis have been unable to achieve high spatial resolution (1-5 um) required. In this study, we focused on imaging, visualization, and analysis of early liver development. We utilized available online databases for both mouse (EMAP) and human (3D Atlas of Human Embryology) liver development. To visualize liver bud morphogenesis at high spatial resolution, we performed 3D reconstructions of stacked, digital tissue sections. We show dynamic 3D hepatic cord formation in the mouse in humans. Interestingly, when we quantified fetal liver growth, we showed that 3D fetal liver growth appears to occur in spurts rather continuously, and 3D images suggest that there could be considerable remodeling during these stages. Further, our analysis of the STM, in both mouse and humans, demonstrates that it increases in size during early fetal liver growth, that is highly interconnecting with liver epithelium, and that it can have strong local effects on growth. Finally, we identify and visualize and identify human hepatic cord formation followed by rapid sheet-like growth, which we propose could be an under-appreciated morphological feature that enables rapid growth of early human fetal liver. These studies will motivate future approaches to employ in vitro culture and organoid technology to improve human PSC differentiation, and improve disease modeling, and therapeutic opportunities for liver diseases. In conclusion, compared to 2D sectioning, high spatial resolution imaging of the mouse and human 3D liver bud morphogenesis enables greatly improved visualization of the hepatic cords, 3D sheet-like liver cell growth, STM-epithelial cell interactions, and quantitative comparisons between mouse and human liver bud morphogenesis.

Promoter-specific IGF2 imprinting status and its plasticity during human liver development

Development ◽  
1995 ◽  
Vol 121 (2) ◽  
pp. 309-316 ◽  
Author(s):  
T.J. Ekstrom ◽  
H. Cui ◽  
X. Li ◽  
R. Ohlsson
Keyword(s):  
Postnatal Development ◽  
Human Liver ◽  
Expression Patterns ◽  
Liver Development ◽  
Maternal Allele ◽  
Adult Liver ◽  
Biallelic Expression ◽  
Prenatal And Postnatal Development

IGF2 has been shown to be expressed preferentially from the paternally derived allele, although the maternal allele can be found active during both prenatal and postnatal development as well as in neoplastic tumours in humans. We addressed here whether or not the biallelic expression patterns that can be seen during postnatal human liver development reflected a coordinated change in the activities of the four promoters of human IGF2. We show here that the P2, P3 and P4 promoters, but not the P1 promoter, display monoallelic activity in embryonic, neonatal and younger infant liver specimens. The P2, P3 and P4 promoters can, however, be found active either monoallelically or biallelically or even monoallelically on opposite parental alleles in older infant and adult liver specimens. In contrast, H19, which is closely linked to IGF2, is monoallelically expressed in all postnatal liver samples analysed. We conclude that the functional imprinting status of IGF2 during postnatal liver development appears to be promoter/enhancer-specific and either partly or completely independent of H19.

693 THE CELL SOURCES AND DIFFERENTIATION OF INTRAHEPATIC BILE DUCTS DURING HUMAN LIVER DEVELOPMENT

2011 ◽  
Vol 54 ◽  
pp. S278-S279
Author(s):  
A. Gumerova ◽  
A. Kiassov ◽  
M. Titova ◽  
S. Abdulkhakov ◽  
M. Kaligin ◽  
...  
Keyword(s):  
Human Liver ◽  
Bile Ducts ◽  
Liver Development ◽  
Intrahepatic Bile Ducts ◽  
Cell Sources

scholarly journals Epithelial expression of angiogenic growth factors modulate arterial vasculogenesis in human liver development

Hepatology ◽  
2007 ◽  
Vol 47 (2) ◽  
pp. 719-728 ◽  
Author(s):  
Luca Fabris ◽  
Massimiliano Cadamuro ◽  
Louis Libbrecht ◽  
Peggy Raynaud ◽  
Carlo Spirlì ◽  
...  
Keyword(s):  
Growth Factors ◽  
Human Liver ◽  
Liver Development ◽  
Angiogenic Growth Factors

Identification of bipotential progenitor cells in human liver development

1995 ◽  
Vol 108 (4) ◽  
pp. A1161 ◽  
Author(s):  
K. Saito ◽  
S. Spaulding ◽  
Y. Haruna ◽  
M.A. Nalesnik ◽  
M.A. Gerber
Keyword(s):  
Progenitor Cells ◽  
Human Liver ◽  
Liver Development
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